Multiplexed ELISA
Why use Multiplexed ELISAs?
Multiplexing is shown to be more efficient with time, cost, and sample. However, there are multiple formats that can be multiplexed. The Quansys Array is applied in 96 well plates whereas other technologies employ membranes, chips or even bead based arrays. Below is a comparison chart of these different methodologies.
| Membrane/ Chip | Bead Based | 96 Well Plate | |
|---|---|---|---|
| Sample Size | 1 ml per test | 12.5-50 µl / test | 5-30 µl / test |
| Cost Per Data Point | $3.30 per data point | $1.70 per data point | $0.71 per data point |
| Speed- Data Point per Test | 46 data points / test | 1000 data points / test | 1536 data points / test |
| Protocol | Similar to Western Blots | Complex- New Application | Similar to ELISA |
| Equipment | Gel Doc Systems or Auto Radiography ($1-40K) | Equipment ($100-200K) | Gel Doc System ($7-40K) |
| Automation | Not Capable | Capable | Capable |
| Variation | Acceptable Variability ≤10% CV | Lot to Lot Variability | Low Variability ≤7% CV |
| Quantification | Qualitative | Quantitative | Quantitative |
| Clinical Application | No Applications | Multiple Applications | Limited Applications |
List of Supporting Literature
1. Barnard, D. L., C. W. Day, et al. (2006). "Enhancement of the infectivity of SARS-CoV in BALB/c mice by IMP dehydrogenase inhibitors, including ribavirin."
Antiviral Res 71(1): 53-63.
2. de Jager, W. and G. T. Rijkers (2006). "Solid-phase and bead-based cytokine immunoassay: a comparison." Methods 38(4): 294-303.
3. Ekins, R. and P. Edwards (1997). "On the meaning of "sensitivity"." Clin Chem 43(10): 1824-31.
4. Lash, G. E., P. J. Scaife, et al. (2006). "Comparison of three Multiplexed cytokine analysis systems: Luminex, SearchLight and FAST Quant." J Immunol
Methods 309(1-2): 205-8.
5. Wiese, R., Y. Belosludtsev, et al. (2001). "Simultaneous multianalyte ELISA performed on a microarray platform." Clin Chem 47(8): 1451-7.
Applications
The Quansys Biosciences Array has been tested with variety of samples. Sera, plasma, tissue culture homegenates, cell culture supernates, lysates, nasal lavage and urine are some of the samples that we have tested.
