Compare Multiplex Platforms
There are three main technologies utilizing the multiplexing format. The Q-Plex™ Technology is a planar-based format in 96-well plates whereas other technologies employ costly electro-chemiluminescent or complex bead-based arrays. Below is a comparison chart compiled from public information on these different methodologies.
Q-Plex™ Technology | Electro-Chemiluminescent | Bead-Based | |
---|---|---|---|
Time | 2100 data points / test | 960 data points / test | >5000 data points / test |
Sample Size | 15-50 µl / test | 50-100 µl / test | 12.5-50 µl / test |
Cost | $1,318 (9-Plex) | $1,519 (10-Plex) | $2,002 (9-Plex) |
Protocol | Similar to ELISA | Complex-electronic chip in 96 well plate | Complex-microfluidic channels |
Equipment | Q-View Imager ($16-38K) | Equipment ($60-250K) | Equipment ($50-200K) |
Sample Types | Many types | Many types | Problems with serum and other viscous samples |
Variation | Acceptable Variability ≤10% CV | Acceptable Variability ≤10% CV | Bead-lot to lot variability |
Cost Per Data Point | $0.85 per data point | $1.58 per data point | $2.02 per data point |
Sensitivity | Low pg/mL | Low pg/mL | Low pg/mL |
High Throughput | Possible | Possible | Not Possible |