Human Cytokine High Sensitivity Screen (15-Plex)

Product at a Glance

Name: Human Cytokine HS Screen (15-Plex)

Catalog: #112449HU

Kit Type: Multiplex ELISA

Multiplex Format: Antibody Array

Detection Method: Chemiluminescent

Analytes: IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-13, IL-15, IL-17, IL-23, IFN-γ, TNF-α, TNF-β

Product Overview

The Q-Plex™ Human Cytokine HS Screen (15-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 15 biomarkers or analytes. Using less than 50 µl of sample per well, up to 80 samples can be assayed for all 15 markers in the panel within 2.5 hours. The Q-Plex™ Human Cytokine HS Screen (15-Plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Q-Plex plates are built by adsorbing 15 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.

Kit Content

Each kit contains a 96-well plate, featuring the relevant biomarker panel in each well, and all reagents required to perform testing.

Reagents include:

  • Calibrator
  • Detection mix
  • Substrate
  • Sample diluents
  • Streptavidin HRP
  • Wash buffer
Assay Ranges
Units Calibrator Range LLD Limit
IFN-γ pg/ml 80 - 0.11 0.10
IL-1α pg/ml 1300 - 1.78 1.40
IL-1β pg/ml 3070 - 4.21 1.75
IL-2 pg/ml 50 - 0.07 0.07
IL-4 pg/ml 23 - 0.03 0.02
IL-5 pg/ml 360 - 0.49 0.20
IL-6 pg/ml 350 - 0.48 0.40
IL-10 pg/ml 950 - 1.30 1.10
IL-12p70 pg/ml 3000 - 4.12 3.00
IL-13 pg/ml 340 - 0.47 0.23
IL-15 pg/ml 950 - 1.30 0.43
IL-17 pg/ml 1200 - 1.65 1.48
IL-23 pg/ml 9000 - 12.35 1.50
TNFα pg/ml 440 - 0.60 0.31
TNFβ pg/ml 470 - 0.64 0.57

Nitric Oxide Synthase Inhibition Modulates Immune Polarization and Improves Radiation-Induced Tumor Growth Delay

  • Authors: Lisa A Ridnour, Robert YS Cheng, Jonathan M Weiss, et al.
  • The Journal of Cancer Research, 2015. doi: 10.1158/0008-5472.CAN-14-3011

miR-146a and miR-146b Regulate Human Dendritic Cell Apoptosis and Cytokine Production by Targeting of TRAF6 and IRAK1

  • Authors: Haein Park, Xin Huang, Changming Lu, et al.
  • J. Biol. Chem. published online December 11, 2014 doi: 10.1074/jbc.M114.591420

The Anti-Inflammatory Activity of a Novel Fused-Cyclopentenone Phosphonate and Its Potential in the Local Treatment of Experimental Colitis

  • Authors: Dorit Moradov, Helena Shifrin, Efrat Harel, et al.
  • Gastroenterology Research and Practice, vol. 2015, Article ID 939483, doi:10.1155/2015/939483

Serum cytokines as biomarkers for age-related macular degeneration

  • Authors: Khaled Nassar, Salvatore Grisanti, Elshaymaa Elfar, et al.
  • Graefe’s Archive for Clinical and Experimental Ophthalmology, July 2014

Short Report: Association of IL-15 with Peripheral and Hepatic Insulin Sensitivity in Healthy Middle-Aged Men

  • Authors: Yuichiro Nishida, Kumpei Tokuyama, et al.
  • Open Journal of Endocrine and Metabolic Diseases Vol.4 No.3(2014), Article ID:43974,9 pages
Assay Ranges
Units Calibrator Range LLD Limit
IFN-γ pg/ml 80 - 0.11 0.10
IL-1α pg/ml 1300 - 1.78 1.40
IL-1β pg/ml 3070 - 4.21 1.75
IL-2 pg/ml 50 - 0.07 0.07
IL-4 pg/ml 23 - 0.03 0.02
IL-5 pg/ml 360 - 0.49 0.20
IL-6 pg/ml 350 - 0.48 0.40
IL-10 pg/ml 950 - 1.30 1.10
IL-12p70 pg/ml 3000 - 4.12 3.00
IL-13 pg/ml 340 - 0.47 0.23
IL-15 pg/ml 950 - 1.30 0.43
IL-17 pg/ml 1200 - 1.65 1.48
IL-23 pg/ml 9000 - 12.35 1.50
TNFα pg/ml 440 - 0.60 0.31
TNFβ pg/ml 470 - 0.64 0.57

Nitric Oxide Synthase Inhibition Modulates Immune Polarization and Improves Radiation-Induced Tumor Growth Delay

  • Authors: Lisa A Ridnour, Robert YS Cheng, Jonathan M Weiss, et al.
  • The Journal of Cancer Research, 2015. doi: 10.1158/0008-5472.CAN-14-3011

miR-146a and miR-146b Regulate Human Dendritic Cell Apoptosis and Cytokine Production by Targeting of TRAF6 and IRAK1

  • Authors: Haein Park, Xin Huang, Changming Lu, et al.
  • J. Biol. Chem. published online December 11, 2014 doi: 10.1074/jbc.M114.591420

The Anti-Inflammatory Activity of a Novel Fused-Cyclopentenone Phosphonate and Its Potential in the Local Treatment of Experimental Colitis

  • Authors: Dorit Moradov, Helena Shifrin, Efrat Harel, et al.
  • Gastroenterology Research and Practice, vol. 2015, Article ID 939483, doi:10.1155/2015/939483

Serum cytokines as biomarkers for age-related macular degeneration

  • Authors: Khaled Nassar, Salvatore Grisanti, Elshaymaa Elfar, et al.
  • Graefe’s Archive for Clinical and Experimental Ophthalmology, July 2014

Short Report: Association of IL-15 with Peripheral and Hepatic Insulin Sensitivity in Healthy Middle-Aged Men

  • Authors: Yuichiro Nishida, Kumpei Tokuyama, et al.
  • Open Journal of Endocrine and Metabolic Diseases Vol.4 No.3(2014), Article ID:43974,9 pages