Human Cytokine High Sensitivity (9-Plex)

Product at a Glance

Name: Q-Plex™ Human Cytokine HS (9-Plex)

Catalog: #114449HU

Kit Type: Multiplex ELISA

Multiplex Format: Antibody Array

Detection Method: Chemiluminescent

Analytes: IL-1α, IL-1β, IL-4, IL-5, IL-6, IL-10, IL-17, IFN-γ, TNF-α

Product Overview

The Q-Plex™ Human Cytokine HS (9-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 9 biomarkers or analytes. Using less than 50 µl of sample per well, up to 80 samples can be assayed for all 9 markers in the panel within 2.5 hours. The Q-Plex™ Human Cytokine HS (9-Plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Q-Plex plates are built by adsorbing 9 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.

Kit Content

Each kit contains a 96-well plate, featuring the relevant biomarker panel in each well, and all reagents required to perform testing.

Reagents include:

  • Calibrator
  • Detection mix
  • Substrate
  • Sample diluents
  • Streptavidin HRP
  • Wash buffer
Assay Ranges
Units Calibrator Range LLD Limit
IL-1α pg/ml 1300 – 1.78 1.4
IL-1β pg/ml 3070 – 4.21 1.75
IL-4 pg/ml 23 – 0.03 0.02
IL-5 pg/ml 360 – 0.49 0.2
IL-6 pg/ml 350 – 0.48 0.4
IL-10 pg/ml 950 – 1.30 1.1
IL-17 pg/ml 1200 – 1.65 1.48
IFNγ pg/ml 80 – 0.11 0.1
TNFα pg/ml 440 – 0.60 0.31

Lutein and β-Cryptoxanthin Inhibit Inflammatory Mediators in Human Chondrosarcoma Cells Induced with IL-1β

  • Authors: Michael M. Di Filippo, Bridget D. Mathison, Jean Soon Park and Boon P. Chew
  • Open Nutrition Journal (2012), 6, 41-47

Stress management skills, neuroimmune processes and fatigue levels in persons with chronic fatigue syndrome

  • Authors: Lattie, Emily G., Antoni, Michael H.
  • Brain, Behavior, and Immunity

Plasmodium falciparum merozoite surface protein 1 blocks the proinflammatory protein S100P

  • Authors: Waisberg M, Cerqueira G, Yager S, Francischetti I, et al.
  • PNAS (February 2012) 109 (14) 5429–5434

Molecular and Cellular Characterization of a Salmonella enterica Serovar Paratyphi A Outbreak Strain and the Human Immune Response to its Infection

  • Authors: Ohad Gal-Mor, Jotham Suez, Dana Elhadad, et al.
  • Clinical and Vaccine Immunology (February 2012), 2, 146-156
Assay Ranges
Units Calibrator Range LLD Limit
IL-1α pg/ml 1300 – 1.78 1.4
IL-1β pg/ml 3070 – 4.21 1.75
IL-4 pg/ml 23 – 0.03 0.02
IL-5 pg/ml 360 – 0.49 0.2
IL-6 pg/ml 350 – 0.48 0.4
IL-10 pg/ml 950 – 1.30 1.1
IL-17 pg/ml 1200 – 1.65 1.48
IFNγ pg/ml 80 – 0.11 0.1
TNFα pg/ml 440 – 0.60 0.31

Lutein and β-Cryptoxanthin Inhibit Inflammatory Mediators in Human Chondrosarcoma Cells Induced with IL-1β

  • Authors: Michael M. Di Filippo, Bridget D. Mathison, Jean Soon Park and Boon P. Chew
  • Open Nutrition Journal (2012), 6, 41-47

Stress management skills, neuroimmune processes and fatigue levels in persons with chronic fatigue syndrome

  • Authors: Lattie, Emily G., Antoni, Michael H.
  • Brain, Behavior, and Immunity

Plasmodium falciparum merozoite surface protein 1 blocks the proinflammatory protein S100P

  • Authors: Waisberg M, Cerqueira G, Yager S, Francischetti I, et al.
  • PNAS (February 2012) 109 (14) 5429–5434

Molecular and Cellular Characterization of a Salmonella enterica Serovar Paratyphi A Outbreak Strain and the Human Immune Response to its Infection

  • Authors: Ohad Gal-Mor, Jotham Suez, Dana Elhadad, et al.
  • Clinical and Vaccine Immunology (February 2012), 2, 146-156