Human Cytokine – Screen (16-plex)

Product at a Glance

Name: Human Cytokine – Screen (16-plex)

Catalog: #110949HU

Kit Type: Multiplex ELISA

Multiplex Format: Antibody Array

Detection Method: Chemiluminescent

Analytes: IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-15, IL-17, IL-23, IFNγ, TNFα, TNFβ

Product Overview

The Q-Plex™ Human Cytokine – Screen (16-plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 16 biomarkers or analytes. Multiple analyte measurements are achieved by adsorbing 16 distinct capture antibodies in a defined array to the bottom of each well of a 96-well plate. Our high-quality reagents help ensure the accuracy and precision of your results.

Features and Benefits

Using less than 50 µl of sample per well, up to 80 samples can be assayed for all 16 markers in the panel within 2.5 hours. The Q-Plex™ Human Cytokine – Screen (16-plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample.

Kit Content

Each kit contains a 96-well plate, featuring the relevant biomarker panel in each well, and all reagents required to perform testing.

Reagents include:

  • Calibrator
  • Detection mix
  • Substrate
  • Sample diluents
  • Streptavidin HRP
  • Wash buffer
Assay Ranges
Units Calibrator Range LLD Limit
IL-1α pg/ml 4000 – 5.49 5.43
IL-1β pg/ml 10000 - 13.72 10.14
IL-2 pg/ml 4000 – 5.49 3.16
IL-4 pg/ml 2000 – 2.74 2.14
IL-5 pg/ml 3000 – 4.12 2.90
IL-6 pg/ml 3000 – 4.11 3.47
IL-8 pg/ml 2000 – 2.74 2.70
IL-10 pg/ml 3000 – 4.11 4.10
IL-12p70 pg/ml 3000 – 4.12 3.20
IL-13 pg/ml 3000 – 4.12 2.86
IL-15 pg/ml 3000 – 4.12 1.92
IL-17 pg/ml 4000 - 5.49 5.40
IL-23 pg/ml 30000 - 41.15 25.00
IFNγ pg/ml 8000 - 10.97 4.95
TNFα pg/ml 4000 - 5.49 5.20
TNFβ pg/ml 4000 - 5.49 5.29

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Assay Ranges
Units Calibrator Range LLD Limit
IL-1α pg/ml 4000 – 5.49 5.43
IL-1β pg/ml 10000 - 13.72 10.14
IL-2 pg/ml 4000 – 5.49 3.16
IL-4 pg/ml 2000 – 2.74 2.14
IL-5 pg/ml 3000 – 4.12 2.90
IL-6 pg/ml 3000 – 4.11 3.47
IL-8 pg/ml 2000 – 2.74 2.70
IL-10 pg/ml 3000 – 4.11 4.10
IL-12p70 pg/ml 3000 – 4.12 3.20
IL-13 pg/ml 3000 – 4.12 2.86
IL-15 pg/ml 3000 – 4.12 1.92
IL-17 pg/ml 4000 - 5.49 5.40
IL-23 pg/ml 30000 - 41.15 25.00
IFNγ pg/ml 8000 - 10.97 4.95
TNFα pg/ml 4000 - 5.49 5.20
TNFβ pg/ml 4000 - 5.49 5.29

Introducing MARCo: Histoserological Findings of a Multi-Organic Paraneoplastic Syndrome in Cutaneous Melanoma Patients

  • Authors: Ranjbar, M., Mohi, A., Pföhler, C. et al.
  • Dermatology and Therapy. (2016) DOI: 10.1007/s13555-016-0142-5

Paclitaxel-resistant cancer cell-derived secretomes elicit ABCB1-associated docetaxel cross-resistance and escape from apoptosis through FOXO3a-driven glycolytic regulation

  • Authors: Mark Borris D Aldonza, Ji-Young Hong, and Sang Kook Lee
  • Experimental & Molecular Medicine 2017: doi.org/10.1038/emm.2016.131

Serum cytokines as biomarkers for age-related macular degeneration

  • Authors: Khaled Nassar, Salvatore Grisanti, Elshaymaa Elfar, et al.
  • Graefe’s Archive for Clinical and Experimental Ophthalmology. May 2015, Volume 253, Issue 5, pp 699-704

Intranasal delivery of a protein subunit vaccine using a Tobacco Mosaic Virus platform protects against pneumonic plague

  • Authors: Paul M. Arnaboldi, Mariya Sambir, Christina D’Arco, et al.
  • Vaccine 2016: http://dx.doi.org/10.1016/ j.vaccine.2016.09.063

An elevated pro-inflammatory cytokines profile in Behcet’s disease: A multiplex analysis

  • Authors: Nasser Gholijani, Mohammad Reza Ataollahi, Afshin Samiei, et al.
  • Immunology Letters. 2016: doi.org/10.1016/ j.imlet.2016.12.001

IGF1R- and ROR1-Specific CAR T Cells as a Potential Therapy for High Risk Sarcomas

  • Authors: Xin Huang, Haein Park, Joseph Greene, et al.
  • Plos One: 2015, DOI: 10.1371/journal.pone.0133152

Illness progression in chronic fatigue syndrome: a shifting immune baseline

  • Authors: Lindsey Russell, Gordon Broderick, Renee Taylor, et al.
  • BMC Immunology201617:3 DOI: 10.1186/s12865-016-0142-3

The Activation-Induced Assembly of an RNA/Protein Interactome Centered on the Splicing Factor U2AF2 Regulates Gene Expression in Human CD4 T Cells

  • Authors: Thomas Whisenant, Eigen Peralta, Lauren Aarreberg, et al.
  • Plos One: 2015. DOI: 10.1371/journal.pone.0144409

Defining CD4 T Cell Memory by the Epigenetic Landscape of CpG DNA Methylation

  • Authors: H. Kiyomi Komori, Traver Hart, Sarah A. LaMere, et al.
  • The Journal of Immunology February 15, 2015, doi: 10.4049/ jimmunol.1401162