Human Cytokine – Stripwells (16-plex)

Product at a Glance

Name: Human Cytokine – Stripwells (16-plex)

Catalog: #110349HU

Kit Type: Multiplex ELISA

Multiplex Format: Antibody Array

Detection Method: Chemiluminescent

Analytes: IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-15, IL-17, IL-23, IFNγ, TNFα, TNFβ

Product Overview

The Q-Plex™ Human Cytokine – Stripwells (16-plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 16 biomarkers or analytes. Using less than 50 µl of sample per well, up to 80 samples can be assayed for all 16 markers in the panel within 2.5 hours. The Q-Plex™ Human Cytokine HS Screen (15-Plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Q-Plex plates are built by adsorbing 16 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.

Kit Content

Each kit contains a 96-well plate, featuring the relevant biomarker panel in each well, and all reagents required to perform testing.

Reagents include:

  • Calibrator
  • Detection mix
  • Substrate
  • Sample diluents
  • Streptavidin HRP
  • Wash buffer
Assay Ranges
Units Calibrator Range LLD Limit
IL-1α pg/ml 4000 – 5.49 5.43
IL-1β pg/ml 10000 - 13.72 10.14
IL-2 pg/ml 4000 – 5.49 3.16
IL-4 pg/ml 2000 – 2.74 2.14
IL-5 pg/ml 3000 – 4.12 2.90
IL-6 pg/ml 3000 – 4.11 3.47
IL-8 pg/ml 2000 – 2.74 2.70
IL-10 pg/ml 3000 – 4.11 4.10
IL-12p70 pg/ml 3000 – 4.12 3.20
IL-13 pg/ml 3000 – 4.12 2.86
IL-15 pg/ml 3000 – 4.12 1.92
IL-17 pg/ml 4000 - 5.49 5.40
IL-23 pg/ml 30000 - 41.15 25.00
IFNγ pg/ml 8000 - 10.97 4.95
TNFα pg/ml 4000 - 5.49 5.20
TNFβ pg/ml 4000 - 5.49 5.29

Hypothetical Link Between Osteopathic Suboccipital Decompression and Neuroimmunomodulation

  • Authors: Joshua A Cuoco, Charles N Fennie and George K Cheriyan
  • Journal of Neurology and Neuroscience 2016

The Effects of Telephone-Delivered Cognitive Behavioral Stress Management on Inflammation and Symptoms in Myalgic Enchephalomyelitis/Chronic Fatigue Syndrome: A Computational Immunology Approach

  • Authors: Emily G. Lattie
  • Open Access Dissertations, 2015. Paper 1445. http://scholarlyrepository.miami. edu/oa_dissertations/1445

Altered Serum Cytokine Profiles in Relapse Phase of Relapsing-Remitting Multiple Sclerosis

  • Authors: Forooz Peiravian, Hamid Rajaian, Afshin Samiei, et al.
  • Iranian Journal of Immunology. 2016.

Defining CD4 T Cell Memory by the Epigenetic Landscape of CpG DNA Methylation

  • Authors: H. Kiyomi Komori, Traver Hart, Sarah A. LaMere, et al.
  • The Journal of Immunology February 15, 2015, doi: 10.4049/ jimmunol.1401162

A 4-trifluoromethyl analogue of celecoxib inhibits arthritis by suppressing innate immune cell activation

  • Authors: Chiba, Asako., Mizuno, Miho., et al
  • Arthritis Research & Therapy
Assay Ranges
Units Calibrator Range LLD Limit
IL-1α pg/ml 4000 – 5.49 5.43
IL-1β pg/ml 10000 - 13.72 10.14
IL-2 pg/ml 4000 – 5.49 3.16
IL-4 pg/ml 2000 – 2.74 2.14
IL-5 pg/ml 3000 – 4.12 2.90
IL-6 pg/ml 3000 – 4.11 3.47
IL-8 pg/ml 2000 – 2.74 2.70
IL-10 pg/ml 3000 – 4.11 4.10
IL-12p70 pg/ml 3000 – 4.12 3.20
IL-13 pg/ml 3000 – 4.12 2.86
IL-15 pg/ml 3000 – 4.12 1.92
IL-17 pg/ml 4000 - 5.49 5.40
IL-23 pg/ml 30000 - 41.15 25.00
IFNγ pg/ml 8000 - 10.97 4.95
TNFα pg/ml 4000 - 5.49 5.20
TNFβ pg/ml 4000 - 5.49 5.29

Hypothetical Link Between Osteopathic Suboccipital Decompression and Neuroimmunomodulation

  • Authors: Joshua A Cuoco, Charles N Fennie and George K Cheriyan
  • Journal of Neurology and Neuroscience 2016

The Effects of Telephone-Delivered Cognitive Behavioral Stress Management on Inflammation and Symptoms in Myalgic Enchephalomyelitis/Chronic Fatigue Syndrome: A Computational Immunology Approach

  • Authors: Emily G. Lattie
  • Open Access Dissertations, 2015. Paper 1445. http://scholarlyrepository.miami. edu/oa_dissertations/1445

Altered Serum Cytokine Profiles in Relapse Phase of Relapsing-Remitting Multiple Sclerosis

  • Authors: Forooz Peiravian, Hamid Rajaian, Afshin Samiei, et al.
  • Iranian Journal of Immunology. 2016.

Defining CD4 T Cell Memory by the Epigenetic Landscape of CpG DNA Methylation

  • Authors: H. Kiyomi Komori, Traver Hart, Sarah A. LaMere, et al.
  • The Journal of Immunology February 15, 2015, doi: 10.4049/ jimmunol.1401162

A 4-trifluoromethyl analogue of celecoxib inhibits arthritis by suppressing innate immune cell activation

  • Authors: Chiba, Asako., Mizuno, Miho., et al
  • Arthritis Research & Therapy