Human Obesity (7-plex)

Product at a Glance

Name: Q-Plex™ Human Obesity (7-Plex)

Catalog: #119049HU

Kit Type: Multiplex ELISA

Multiplex Format: Antibody Array

Detection Method: Chemiluminescent

Analytes: Adipsin, Glucagon, PAI-1, VEGF, RBP4, Ghrelin, Leptin

Product Overview

The Q-Plex Obesity Array (7-plex) is a fully quantitative ELISA-based chemiluminescent assay allowing concurrent measurement of seven biomarkers or analytes (Adipsin, Glucagon, PAI-1, VEGF, RBP4, Ghrelin, and Leptin).

Each kit contains a 96-well plate, with each well featuring the relevant biomarker panel, and all the reagents required to perform testing. Q-Plex plates are built by absorbing seven distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.

Using just 50 µl of sample per well, up to 80 samples can be assayed for all seven markers in the panel in 2.5 hours. Q-Plex Arrays provide scientists with an easy-to-use and cost-effective means of generating a cytokine profile for each sample.

Kit Content

Each kit contains a 96-well plate, featuring the relevant biomarker panel in each well, and all reagents required to perform testing.

Reagents include:

  • Calibrator
  • Detection mix
  • Substrate
  • Sample diluents
  • Wash buffer
Assay Ranges
Units Calibrator Range LLD Limit
Adipsin ug/ml 16.6 - 0.02 0.017
Glucagon pg/ml 2500 - 3.4 2.63
PAI-1 pg/ml 3000 - 4.1 2.75
VEGF pg/ml 5500 - 7.544582 5.18
RBP4 ug/ml 100 - 0.14 0.094
Ghrelin pg/ml 2300 - 3.2 2.19
Leptin pg/ml 3400 - 4.7 3.91

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Assay Ranges
Units Calibrator Range LLD Limit
Adipsin ug/ml 16.6 - 0.02 0.017
Glucagon pg/ml 2500 - 3.4 2.63
PAI-1 pg/ml 3000 - 4.1 2.75
VEGF pg/ml 5500 - 7.544582 5.18
RBP4 ug/ml 100 - 0.14 0.094
Ghrelin pg/ml 2300 - 3.2 2.19
Leptin pg/ml 3400 - 4.7 3.91

A 22-plex chemiluminescent microarray for pneumococcal antibodies

  • Authors: Pickering, J. W., J. D. Hoopes, et al
  • Am J Clin Pathol128(1): 23-31

Novel inflammatory biomarkers of portal pressure in compensated cirrhosis patients

  • Authors: Martina Buck, Guadalupe Garcia-Tsao, Roberto J. Groszmann, et al.
  • Hepatology Volume 59, Issue 3, pages 1052–1059, March 2014

Profilin-1 haploinsufficiency protects against obesity-associated glucose intolerance and preserves adipose tissue immune homeostasis

  • Authors: Giulio R. Romeo, Munkyong Pae, Jongsoon Lee and Steven E. Shoelson
  • Diabetes July 24, 2013

Genetic susceptibility to systemic lupus erythematosus protects against cerebral malaria in mice

  • Authors: Waisberg, M., T. Tarasenko, et al
  • Proc Natl Acad Sci U S A 108(3): 1122-7