Mouse Cytokine – Inflammation (14-plex)

Product at a Glance

Name: Q-Plex™ Mouse Cytokine – Inflammation (14-plex)

Catalog: #110449MS

Kit Type: Multiplex ELISA

Multiplex Format: Antibody Array

Detection Method: Chemiluminescent

Analytes: IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-6, IL-10, IL-12p70, IL-17, MCP-1,TNFα, MIP-1α, GMCSF, RANTES

Product Overview

The Q-Plex™ Mouse Cytokine – Inflammation (14-plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 14 biomarkers or analytes. Using less than 50 µl of sample per well, up to 80 samples can be assayed for all 14 markers in the panel within 2.5 hours. The Q-Plex™ Mouse Cytokine – Inflammation (14-plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Q-Plex plates are built by adsorbing 14 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.

Kit Content

Each kit contains a 96-well plate, featuring the relevant biomarker panel in each well, and all reagents required to perform testing.

Reagents include:

  • Calibrator
  • Detection mix
  • Substrate
  • Sample diluents
  • Streptavidin HRP
  • Wash buffer
Assay Ranges
UnitsCalibrator RangeLLD Limit
GMCSFpg/ml2000 - 2.742.30
IL-1αpg/ml5000 - 6.864.20
IL-1βpg/ml14000 - 19.2012.41
IL-2pg/ml5000 - 6.863.44
IL-3pg/ml5000 - 6.863.18
IL-4pg/ml5000 - 6.862.50
IL-6pg/ml5000 - 6.862.90
IL-10pg/ml2000 - 2.753.00
IL-12p70pg/ml12000 - 16.4612.20
IL-17pg/ml10000 - 13.726.20
MCP-1pg/ml3000 - 4.123.40
MIP-1αpg/ml5000 - 6.860.61
RANTESpg/ml3000 - 4.122.15
TNFαpg/ml3000 - 4.123.40

Excessive intake of trans fatty acid accelerates atherosclerosis through promoting inflammation and oxidative stress in a mouse model of hyperlipidemia

  • Authors: Tomoko Monguchi, Tetsuya Hara, Minoru Hasokawa, et al.
  • Journal of Cardiology 2017: doi.org/10.1016/j.jjcc.2016.12.012

Macrophage-Derived Chemokine (MDC/CCL22) is a Novel Mediator of Lung Inflammation Following Hemorrhage and Resuscitation

  • Authors: Jillian R. Richter, Jeffrey M. Sutton, Ritha M. Belizaire, et al.
  • Shock. 2014 December ; 42(6): 525–531.

Treatment with chondroitin sulfate to modulate inflammation and atherogenesis in obesity

  • Authors: Pedro Melgar-Lesmesa, Fernando Garcia-Politea, Paula Del-Rey-Puecha, et al.
  • Atherosclerosis. (2016) doi: 10.1016/j.atherosclerosis. 2015.12.016

Damage Control Resuscitation Decreases Systemic Inflammation After Hemorrhage

  • Authors: Makley, Amy T., Goodman, Michael D., et al.
  • Journal of Surgical Research 175, 75-82

Invasive Salmonella Typhimurium ST313 with Naturally Attenuated Flagellin Elicits Reduced Inflammation and Replicates within Macrophages

  • Authors: Girish Ramachandran, Darren J. Perkins, Patrick J. Schmidlein, et al.
  • PLOS 2015, doi: 10.1371/journal.pntd.0003394
Assay Ranges
UnitsCalibrator RangeLLD Limit
GMCSFpg/ml2000 - 2.742.30
IL-1αpg/ml5000 - 6.864.20
IL-1βpg/ml14000 - 19.2012.41
IL-2pg/ml5000 - 6.863.44
IL-3pg/ml5000 - 6.863.18
IL-4pg/ml5000 - 6.862.50
IL-6pg/ml5000 - 6.862.90
IL-10pg/ml2000 - 2.753.00
IL-12p70pg/ml12000 - 16.4612.20
IL-17pg/ml10000 - 13.726.20
MCP-1pg/ml3000 - 4.123.40
MIP-1αpg/ml5000 - 6.860.61
RANTESpg/ml3000 - 4.122.15
TNFαpg/ml3000 - 4.123.40

Excessive intake of trans fatty acid accelerates atherosclerosis through promoting inflammation and oxidative stress in a mouse model of hyperlipidemia

  • Authors: Tomoko Monguchi, Tetsuya Hara, Minoru Hasokawa, et al.
  • Journal of Cardiology 2017: doi.org/10.1016/j.jjcc.2016.12.012

Macrophage-Derived Chemokine (MDC/CCL22) is a Novel Mediator of Lung Inflammation Following Hemorrhage and Resuscitation

  • Authors: Jillian R. Richter, Jeffrey M. Sutton, Ritha M. Belizaire, et al.
  • Shock. 2014 December ; 42(6): 525–531.

Treatment with chondroitin sulfate to modulate inflammation and atherogenesis in obesity

  • Authors: Pedro Melgar-Lesmesa, Fernando Garcia-Politea, Paula Del-Rey-Puecha, et al.
  • Atherosclerosis. (2016) doi: 10.1016/j.atherosclerosis. 2015.12.016

Damage Control Resuscitation Decreases Systemic Inflammation After Hemorrhage

  • Authors: Makley, Amy T., Goodman, Michael D., et al.
  • Journal of Surgical Research 175, 75-82

Invasive Salmonella Typhimurium ST313 with Naturally Attenuated Flagellin Elicits Reduced Inflammation and Replicates within Macrophages

  • Authors: Girish Ramachandran, Darren J. Perkins, Patrick J. Schmidlein, et al.
  • PLOS 2015, doi: 10.1371/journal.pntd.0003394