Mouse Cytokine – Screen (16-Plex)

Product at a Glance

Name: Mouse Cytokine – Screen (16-Plex)

Catalog: #110949MS

Kit Type: Multiplex ELISA

Multiplex Format: Antibody Array

Detection Method: Chemiluminescent

Analytes: IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-17, MCP-1, IFNγ, TNFα, MIP-1α, GMCSF, RANTES

Product Overview

The Q-Plex™ Mouse Cytokine – Screen (16-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 16 biomarkers or analytes. Using less than 50 µl of sample per well, up to 80 samples can be assayed for all 16 markers in the panel within 2.5 hours. The Mouse Cytokine – Screen (16-Plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Q-Plex plates are built by adsorbing 16 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.

Kit Content

Each kit contains a 96-well plate, featuring the relevant biomarker panel in each well, and all reagents required to perform testing.

Reagents include:

  • Calibrator
  • Detection mix
  • Substrate
  • Sample diluents
  • Streptavidin HRP
  • Wash buffer
Assay Ranges
Units Calibrator Range LLD Limit
GMCSF pg/ml 2000 - 2.74 2.30
IFNy pg/ml 8000 - 10.97 5.40
IL-1α pg/ml 5000 - 6.86 4.20
IL-1β pg/ml 14000 - 19.20 12.41
IL-2 pg/ml 5000 - 6.86 3.44
IL-3 pg/ml 5000 - 6.86 3.18
IL-4 pg/ml 5000 - 6.86 2.71
IL-5 pg/ml 5000 - 6.86 2.50
IL-6 pg/ml 5000 - 6.86 2.90
IL-10 pg/ml 2000 - 2.74 3.00
IL-12p70 pg/ml 12000 - 16.46 10.20
IL-17 pg/ml 10000 - 13.72 6.20
MCP-1 pg/ml 3000 - 4.12 3.40
MIP-1α pg/ml 5000 - 6.86 0.61
RANTES pg/ml 3000 - 4.12 2.15
TNFα pg/ml 3000 - 4.12 3.40

Consensus M2e peptide conjugated to gold nanoparticles confers protection against H1N1, H3N2, and H5N1 influenza A viruses

  • Authors: Wenqian Tao, Brett L. Hurst, Akhilesh Kumar Shakya, et al.
  • Antiviral Research 2017: doi.org/10.1016/j.antiviral.2017.01.021

vIL-10-overexpressing human MSCs modulate naïve and activated T lymphocytes following induction of collagenase-induced osteoarthritis

  • Authors: Eric Farrell, Niamh Fahy, Aideen E Ryan, et al.
  • Stem Cell Research & Therapy20167:74 DOI: 10.1186/s13287-016-0331-2

A Novel Role for TL1A/DR3 in Protection against Intestinal Injury and Infection

  • Authors: Li-Guo Jia, Giorgos Bamias, Kristen O. Arseneau, et al.
  • J Immunol. 2016: doi.org/10.4049/jimmunol.1502466

The Morphological and Molecular Changes of Brain Cells Exposed to Direct Current Electric Field Stimulation

  • Authors: Simon Pelletier, Marie Lagace, Isabelle St-Amour, et al.
  • International Journal of Neuropsychopharmacology (2015)

Concentration and ratio of essential fatty acids influences the inflammatory response in lipopolysaccharide challenged mice

  • Authors: K.J. Hintze, J. Tawzera, R.E. Warda
  • Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA) 2016

Effect of Acer tegmentosum bark on atopic dermatitis-like skin lesions in NC/Nga mice

  • Authors: Gabsik Yanga, Duckgun Ana, Mi-Hwa Leea, et al.
  • J Ethnopharmacology. (2016) doi: 10.1016/j.jep.2015.10.033

Comparison of AS03 and Alum on immune responses elicited by A/H3N2 split influenza vaccine in young, mature and aged BALB/c mice

  • Authors: Karen K. Yama, Jyotsana Guptaa, Elizabeth K. Allena, et al.
  • Vaccine. 2016: http://dx.doi.org/10.1016/ j.vaccine.2016.02.012

Amelioration of Chikungunya through Inhibition of the Inflammatory Response

  • Authors: Ashley L. Dagley
  • All Graduate Theses and Dissertations. Paper 4996.

A vaccine consisting of Schistosoma mansoni cathepsin B formulated in Montanide ISA 720 VG induces high level protection against murine schistosomiasis

  • Authors: Alessandra Ricciardi, Kittipos Visitsunthorn, John P. Dalton and Momar Ndao
  • BMC Infectious Diseases. (2016) DOI: 10.1186/s12879-016-1444-z

Iron oxide nanoparticles for use in treating non-infectious inflammatory disorders

  • Authors: Jonathan Leor, Tamar Ben-Mordechai, Shimrit Adutler-Lieber, et al.
  • Ramot At Tel Aviv University Ltd. 2016.
Assay Ranges
Units Calibrator Range LLD Limit
GMCSF pg/ml 2000 - 2.74 2.30
IFNy pg/ml 8000 - 10.97 5.40
IL-1α pg/ml 5000 - 6.86 4.20
IL-1β pg/ml 14000 - 19.20 12.41
IL-2 pg/ml 5000 - 6.86 3.44
IL-3 pg/ml 5000 - 6.86 3.18
IL-4 pg/ml 5000 - 6.86 2.71
IL-5 pg/ml 5000 - 6.86 2.50
IL-6 pg/ml 5000 - 6.86 2.90
IL-10 pg/ml 2000 - 2.74 3.00
IL-12p70 pg/ml 12000 - 16.46 10.20
IL-17 pg/ml 10000 - 13.72 6.20
MCP-1 pg/ml 3000 - 4.12 3.40
MIP-1α pg/ml 5000 - 6.86 0.61
RANTES pg/ml 3000 - 4.12 2.15
TNFα pg/ml 3000 - 4.12 3.40

Consensus M2e peptide conjugated to gold nanoparticles confers protection against H1N1, H3N2, and H5N1 influenza A viruses

  • Authors: Wenqian Tao, Brett L. Hurst, Akhilesh Kumar Shakya, et al.
  • Antiviral Research 2017: doi.org/10.1016/j.antiviral.2017.01.021

vIL-10-overexpressing human MSCs modulate naïve and activated T lymphocytes following induction of collagenase-induced osteoarthritis

  • Authors: Eric Farrell, Niamh Fahy, Aideen E Ryan, et al.
  • Stem Cell Research & Therapy20167:74 DOI: 10.1186/s13287-016-0331-2

A Novel Role for TL1A/DR3 in Protection against Intestinal Injury and Infection

  • Authors: Li-Guo Jia, Giorgos Bamias, Kristen O. Arseneau, et al.
  • J Immunol. 2016: doi.org/10.4049/jimmunol.1502466

The Morphological and Molecular Changes of Brain Cells Exposed to Direct Current Electric Field Stimulation

  • Authors: Simon Pelletier, Marie Lagace, Isabelle St-Amour, et al.
  • International Journal of Neuropsychopharmacology (2015)

Concentration and ratio of essential fatty acids influences the inflammatory response in lipopolysaccharide challenged mice

  • Authors: K.J. Hintze, J. Tawzera, R.E. Warda
  • Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA) 2016

Effect of Acer tegmentosum bark on atopic dermatitis-like skin lesions in NC/Nga mice

  • Authors: Gabsik Yanga, Duckgun Ana, Mi-Hwa Leea, et al.
  • J Ethnopharmacology. (2016) doi: 10.1016/j.jep.2015.10.033

Comparison of AS03 and Alum on immune responses elicited by A/H3N2 split influenza vaccine in young, mature and aged BALB/c mice

  • Authors: Karen K. Yama, Jyotsana Guptaa, Elizabeth K. Allena, et al.
  • Vaccine. 2016: http://dx.doi.org/10.1016/ j.vaccine.2016.02.012

Amelioration of Chikungunya through Inhibition of the Inflammatory Response

  • Authors: Ashley L. Dagley
  • All Graduate Theses and Dissertations. Paper 4996.

A vaccine consisting of Schistosoma mansoni cathepsin B formulated in Montanide ISA 720 VG induces high level protection against murine schistosomiasis

  • Authors: Alessandra Ricciardi, Kittipos Visitsunthorn, John P. Dalton and Momar Ndao
  • BMC Infectious Diseases. (2016) DOI: 10.1186/s12879-016-1444-z

Iron oxide nanoparticles for use in treating non-infectious inflammatory disorders

  • Authors: Jonathan Leor, Tamar Ben-Mordechai, Shimrit Adutler-Lieber, et al.
  • Ramot At Tel Aviv University Ltd. 2016.