Mouse Cytokine Stripwells (16-Plex)

Product at a Glance

Name: Mouse Cytokine Stripwells (16-Plex)

Catalog: #110349MS

Kit Type: Multiplex ELISA

Multiplex Format: Antibody Array

Detection Method: Chemiluminescent

Analytes: IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-17, MCP-1, IFNγ, TNFα, MIP-1α, GMCSF, RANTES

Product Overview

The Q-Plex™ Mouse Cytokine Stripwells (16-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 16 biomarkers or analytes. Using less than 50 µl of sample per well, up to 80 samples can be assayed for all 16 markers in the panel within 2.5 hours. The Q-Plex™ Mouse Cytokine Stripwells (16-Plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Q-Plex plates are built by adsorbing 16 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.

Kit Content

Each kit contains a 96-well plate, featuring the relevant biomarker panel in each well, and all reagents required to perform testing.

Reagents include:

  • Calibrator
  • Detection mix
  • Substrate
  • Sample diluents
  • Streptavidin HRP
  • Wash buffer
Assay Ranges
Units Calibrator Range LLD Limit
IL-1α pg/ml 5000 - 6.86 4.20
IL-1β pg/ml 14000 - 19.20 12.41
IL-2 pg/ml 5000 - 6.86 3.44
IL-3 pg/ml 5000 - 6.86 3.18
IL-4 pg/ml 5000 - 6.86 2.71
IL-5 pg/ml 5000 - 6.86 2.50
IL-6 pg/ml 5000 - 6.86 2.90
IL-10 pg/ml 2000 - 2.74 3.00
IL-12p70 pg/ml 12000 - 16.46 10.20
IL-17 pg/ml 10000 - 13.72 6.20
MCP-1 pg/ml 3000 - 4.12 3.40
IFNy pg/ml 8000 - 10.97 5.40
TNFα pg/ml 3000 - 4.12 3.40
MIP-1α pg/ml 5000 - 6.86 0.61
GMCSF pg/ml 2000 - 2.74 2.30
RANTES pg/ml 3000 - 4.12 2.15

Lymphocytes from chronically stressed mice confer antidepressant-like effects to naive mice

  • Authors: Brachman RA, Lehmann ML, Maric D, Herkenham M
  • J Neurosci. 2015, doi: 10.1523/JNEUROSCI.2278-14.2015
Assay Ranges
Units Calibrator Range LLD Limit
IL-1α pg/ml 5000 - 6.86 4.20
IL-1β pg/ml 14000 - 19.20 12.41
IL-2 pg/ml 5000 - 6.86 3.44
IL-3 pg/ml 5000 - 6.86 3.18
IL-4 pg/ml 5000 - 6.86 2.71
IL-5 pg/ml 5000 - 6.86 2.50
IL-6 pg/ml 5000 - 6.86 2.90
IL-10 pg/ml 2000 - 2.74 3.00
IL-12p70 pg/ml 12000 - 16.46 10.20
IL-17 pg/ml 10000 - 13.72 6.20
MCP-1 pg/ml 3000 - 4.12 3.40
IFNy pg/ml 8000 - 10.97 5.40
TNFα pg/ml 3000 - 4.12 3.40
MIP-1α pg/ml 5000 - 6.86 0.61
GMCSF pg/ml 2000 - 2.74 2.30
RANTES pg/ml 3000 - 4.12 2.15

Lymphocytes from chronically stressed mice confer antidepressant-like effects to naive mice

  • Authors: Brachman RA, Lehmann ML, Maric D, Herkenham M
  • J Neurosci. 2015, doi: 10.1523/JNEUROSCI.2278-14.2015