Performing Western and Dot Blots on the Q-View Imager

While the Q-View Imager was exclusively designed and developed to be used with Q-Plex Arrays, some customers have been successful in using the device for western and dot blot imaging. Due to the numerous methods and reagents available, we are not able to provide specific steps on how to do this. Keep in mind, customers will have to optimize their methods to the imager. Notwithstanding, we can provide a few tips so if you do choose to use the Q-View Imager for this purpose, you’ll have a better chance of success.

Substrate

There are numerous chemiluminescent substrates available on the market of a variety of quality and performance. The substrate used in the Q-Plex kits is very sensitive and stable. The customers that have had good success with the Q-View Imager have used this substrate. From what we have observed, some of the less sensitive substrates are not acceptable for this application. Please contact your sales representative to learn more about where you can purchase this substrate.

Exposure

The camera used in the Q-View Imager is able to do long exposures. We recommend doing multiple exposures, some short and some longer. It may be necessary to do exposures up to five minutes or longer.

Streptavidin HRP (SHRP)

While every experiment is different we recommend maximizing the concentration of SHRP to achieve desired sensitivity.

Examples of Customers Images

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About Us

"We are dedicated to the development of protein arrays that aid researchers and clinicians in better understanding, diagnosing, and treating disease. Our products and services provide value to customers by improving the accuracy and efficiency of their testing."

Quality

Quansys Biosciences is an ISO 9001 and ISO 13485 registered company. We pride ourselves in the quality of our product and our commitment to customer satisfaction.


Quansys Bioscience
Fax: 435-750-6869
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