Mouse Cytokine Screen – IR (16 Plex)

Product Information Sheet (PDF)
MSDS Information

Catalog #130951MS

The Q-Plex™ Mouse Cytokine – IR (16-plex) is a fully quantitative ELISA-based test where 16 distinct capture antibodies have been absorbed to each well of a 96-well plate in a defined array. Using less than 30 µl of sample per well, up to 80 samples can be assayed for all 16 markers in less than 5 hours. The Q-Plex™ Mouse Cytokine – IR (16-plex) provides researchers an easy to use and cost effective means of generating a cytokine profile for each sample. The array is analyzed using the LI-COR Odyssey and Aerius Infrared Imagine Systems.

Many cytokines perform multiple functions and often share the same receptors. A single cytokine can elicit many different effects dependent on the cell type and the other cytokines present. This diversity of activity is what allows the immune system to control a great variety of events with a relatively small repertoire of signaling agents. As a result, the profile of type and concentration of cytokines present at a specific time is critical to receiving the desired function. Because these profiles define what is happening in the immune system, and because of cytokine involvement in both disease pathogenesis and disease recovery, the monitoring of their levels is an invaluable tool in understanding, diagnosing, and monitoring disease.

Range
(pg/ml)
LLD (pg/ml) Standard*
(pg/ml)
IL-1α 5300 – 7 0.44 5300
IL-1β 26000 – 36 4.14 26000
IL-2 5500 – 8 2.59 5500
IL-3 5500 – 8 0.47 5500
IL-4 6300 – 9 0.79 6300
IL-5 8400 – 12 1.94 8400
IL-6 4600 – 6 0.11 4600
IL-10 2600 – 4 0.43 2600
IL-12 9000 – 12 0.60 9000
IL-17 15000 – 21 2.96 15000
MCP-1 13000 – 18 5.08 13000
IFNγ 13000 – 18 0.43 13000
TNFα 6000 – 8 0.05 6000
MIP-1α 8700 – 12 3.89 8700
GMCSF 5800 – 8 0.77 5800
RANTES 13000 – 18 0.66 13000
*The concentration of the Antigen Standard when reconstituted with 300 µl.
This will be the concentration of the high point of the standard curve.

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