Bio Rad Chemi Doc XRS for Quansys Bio multiplex elisa
1. File Setup
1. Open the software on the computer.
2. Click on “File,” then select “ChemiDoc XRS.”
3. Under “Step I-Select Application” press the “Select” button, then select “Custom” and then “Create.”
4. When the new window opens, name this custom setup as “Quansys 1X1 binning.” Under “Illumination” select “none,” and under “gain & binning” select “2X, 1X1” and click “OK.” (Now that this setting is saved you can use it again for future exposures. Instead of selecting “create” select “Quansys 1X1 binning.”)
2. Camera Setup & Focus
1. On the software select “Live Focus.”
2. On the cabinet press the plus button to open the aperture or iris all the way (the lowest number).
3. Open the camera’s drawer, place the plate in the middle of the drawer, then close the drawer.
4. Press the “Epi White” button on the camera cabinet
5. Press the zoom buttons on the cabinet until the plate fills most of the screen on the computer.
6. Replace the plate with the “imaging target sheet” or a mouse pad and close the door
7. Press the focus buttons on the cabinet until the targets or lettering are in focus on the computer screen.
8. Replace the imaging target sheet with the plate, close the drawer, and make sure the plate is in the center of the imaging screen and straight.
9. Turn off the “Epi White” button on the cabinet, and select “freeze” in the software.
3. Image Acquisition
1. On “Step III-Acquire Image” on the software, change the exposure time to 30 seconds and select Manual Expose.
2. When the exposure is complete, convert the image into negative (black background with white spots).
1. Click Image, and new menu appears.
2. Select Transform, and then check the box that says “Invert display.”
3. Click OK.
1. File Setup
1. Open the software on the computer.
2. Click on “File,” then select “ChemiDoc XRS.”
3. Under “Step I-Select Application” press the “Select” button, then select “Custom” and then “Create.”
4. When the new window opens, name this custom setup as “Quansys 1X1 binning.” Under “Illumination” select “none,” and under “gain & binning” select “2X, 1X1” and click “OK.” (Now that this setting is saved you can use it again for future exposures. Instead of selecting “create” select “Quansys 1X1 binning.”)
2. Camera Setup & Focus
1. On the software select “Live Focus.”
2. On the cabinet press the plus button to open the aperture or iris all the way (the lowest number).
3. Open the camera’s drawer, place the plate in the middle of the drawer, then close the drawer.
4. Press the “Epi White” button on the camera cabinet
5. Press the zoom buttons on the cabinet until the plate fills most of the screen on the computer.
6. Replace the plate with the “imaging target sheet” or a mouse pad and close the door
7. Press the focus buttons on the cabinet until the targets or lettering are in focus on the computer screen.
8. Replace the imaging target sheet with the plate, close the drawer, and make sure the plate is in the center of the imaging screen and straight.
9. Turn off the “Epi White” button on the cabinet, and select “freeze” in the software.
3. Image Acquisition
1. On “Step III-Acquire Image” on the software, change the exposure time to 30 seconds and select Manual Expose.
2. When the exposure is complete, convert the image into negative (black background with white spots).
1. Click Image, and new menu appears.
2. Select Transform, and then check the box that says “Invert display.”
3. Click OK.
Save the image.
4. Image Optimization
1. It is recommended you take several images of the plate at different exposure times as each camera, assay, and setup is different. Other suggested image times are 20 seconds, 60 seconds, 90 seconds, and 120 seconds.
5. File Conversion
1. After acquiring the images, you need to convert them to Tiff files.
2. Click on “File” then “Export to Tiff image.”
3. Select “Export raw data,” click on “Export,” then click on “Save.”