Fujifilm LAS 3000 1. File Setup
1. Open the software on the computer.
2. Under “Exposure Type” select “Precision” in the drop down menu.
3. Under “Exposure Time” set the imager to take a 30-second image by selecting “Manual” then entering “30” in the first box and selecting “sec” in the second box.
4. Under “Sensitivity” select “Standard” in the drop down menu.
5. Insure the box next to “Image Acquire & Digitize” is checked.
6. Click on the “Method/Tray Position” button. In the window that appears, select “Chemiluminescence,” and under tray position select “2.” Then select “OK” to close the window.
2. Camera Setup & Focus
1. Open the camera box and make sure the tray is in position “2.”
2. Place the Quansys mouse pad on the tray or other imaging target sheet and close the door.
3. On the software select “Focus” and a new window appears. In the “Adjust” area, click up or down on the arrows until the writing or spots on the mouse pad are in focus.
4. Remove the mouse pad from the imager and place the plate in the center of the tray. Look on the computer screen to make sure the plate is centered and straight in the imaging screen. Close the imager door when the plate is centered.
5. Select the “Return” button on the software to close the focusing window.
3. Image Acquisition
1. When the plate is ready to image, press the “Start” button.
2. After the plate has imaged invert the image to black with white spots by clicking on “View” then selecting “Positive Gray” in the drop down menu.
3. Save the image by pressing the “Save” button. In the new window select “16 bit linear tiff” in the “Save as type” drop down menu. Then type a name for the file and select “Save.”
4. Press the “Complete” button to allow the imager to take another image.
5. Take multiple images at different exposure times to ensure you get the best reading possible. Example exposure times are 20 seconds, 45 seconds, 60 seconds, 90 seconds, and 120 seconds.
1. Open the software on the computer.
2. Under “Exposure Type” select “Precision” in the drop down menu.
3. Under “Exposure Time” set the imager to take a 30-second image by selecting “Manual” then entering “30” in the first box and selecting “sec” in the second box.
4. Under “Sensitivity” select “Standard” in the drop down menu.
5. Insure the box next to “Image Acquire & Digitize” is checked.
6. Click on the “Method/Tray Position” button. In the window that appears, select “Chemiluminescence,” and under tray position select “2.” Then select “OK” to close the window.
2. Camera Setup & Focus
1. Open the camera box and make sure the tray is in position “2.”
2. Place the Quansys mouse pad on the tray or other imaging target sheet and close the door.
3. On the software select “Focus” and a new window appears. In the “Adjust” area, click up or down on the arrows until the writing or spots on the mouse pad are in focus.
4. Remove the mouse pad from the imager and place the plate in the center of the tray. Look on the computer screen to make sure the plate is centered and straight in the imaging screen. Close the imager door when the plate is centered.
5. Select the “Return” button on the software to close the focusing window.
3. Image Acquisition
1. When the plate is ready to image, press the “Start” button.
2. After the plate has imaged invert the image to black with white spots by clicking on “View” then selecting “Positive Gray” in the drop down menu.
3. Save the image by pressing the “Save” button. In the new window select “16 bit linear tiff” in the “Save as type” drop down menu. Then type a name for the file and select “Save.”
4. Press the “Complete” button to allow the imager to take another image.
5. Take multiple images at different exposure times to ensure you get the best reading possible. Example exposure times are 20 seconds, 45 seconds, 60 seconds, 90 seconds, and 120 seconds.