Shop Default

Title:
Mice maintain predominantly maternal Gαs expression throughout life in brown fat tissue (BAT), but not other tissues

Publication:
Bone, 2017: doi.org/10.1016/j.bone.2017.07.001

Author(s):
Olta Tafaj, Steven Hann, Ugur Ayturk, et al.

Quansys Products Used:
Q-View Imager

Abstract:

Show Abstract (218 words)

Title:
High Number of Activated CD8+ T Cells Targeting HIV Antigens Are Present in Cerebrospinal Fluid in Acute HIV Infection

Publication:
JAIDS Journal of Acquired Immune Deficiency Syndromes 2017: doi.org/10.1097/QAI.0000000000001301

Author(s):
Cari F. Kessing, Serena Spudich, Victor Valcour, et al.

Quansys Products Used:
Custom Multiplex Assay and Q-View Software

Abstract:

Show Abstract (78 words)

Title:
Targeting Aurora kinase A and JAK2 prevents GVHD while maintaining Treg and antitumor CTL function

Publication:
Science Translational Medicine 2017: doi.org/10.1126/scitranslmed.aai8269

Author(s):
Brian C. Betts, Anandharaman Veerapathran, Joseph Pidala, et al.

Quansys Products Used:
Multiplex Cytokine Assays

Abstract:

Show Abstract (176 words)

Title:
Defining CD4 T Cell Memory by the Epigenetic Landscape of CpG DNA Methylation

Publication:
Journal of Immunology, 2017: doi.org/10.4049/jimmunol.1401162

Author(s):
H. Kiyomi Komori, Traver Hart, Sarah A. LaMere, et al.

Quansys Products Used:
Q-Plex Human Cytokine Screen

Abstract:

Title:
VCAM-1 Mediates Cigarette Smoke Extract Enhancement of Monocyte Adhesion to Human Carotid Vascular Endothelial Cells

Publication:
KEI Journals, 2017

Author(s):
Gregory B. Pott, Mark Tsurudome, Jamie Bui, et al.

Quansys Products Used:
Custom Multiplex

Abstract:

Show Abstract (145 words)

Title:
The “Aging Factor” Eotaxin-1 (CCL11) Is Detectable in Transfusion Blood Products and Increases with the Donor’s Age

Publication:
Front. Aging Neurosci.,2017 doi.org/10.3389/fnagi.2017.00402

Author(s):
Julia Hoefer, Markus Luger, Christian Dal-Pont, et al.

Quansys Products Used:
Q-Plex Human Immunoassay and Q-View Software

Abstract:

Title:
Immune microenvironment modulation via the interaction of human invariant natural killer T cell (iNKT cells) and Kupffer cells (KC), dendritic cells (DC) in colorectal liver metastasis

Publication:
The Journal of Immunology, 2017

Author(s):
Hao Feng and Wolfgang Thasler

Quansys Products Used:
Quansys Multiplex Array

Abstract:

Show Abstract (260 words)

Title:
Elevated angiopoietin 2 in aqueous of patients with neovascular age related macular degeneration correlates with disease severity at presentation

Publication:
Sci. Rep. 2017: doi.org/10.1038/srep45081

Author(s):
Danny S. Ng, Yolanda W. Yip, Malini Bakthavatsalam, et al.

Quansys Products Used:
Multiplex ELISA Array and Q-View Software

Abstract:

Show Abstract (179 words)

Title:
Precise manipulation of biophysical particle parameters enables control of proinflammatory cytokine production in presence of TLR 3 and 4 ligands

Publication:
Acta Biomaterialia 2017: doi.org/10.1016/j.actbio.2017.01.025

Author(s):
Yoshinori Kakizawa, Jung Seok Lee, Brendan Bell, et al.

Quansys Products Used:
Multiplex ELISA, Mouse cytokine 16-Plex

Abstract:

Show Abstract (183 words)

Title:
A small-molecule IRF3 agonist functions as an influenza vaccine adjuvant by modulating the antiviral immune response

Publication:
Vaccine 2017: doi.org/10.1016/j.vaccine.2017.01.053

Author(s):
Peter Probst, John B. Grigg, Myra Wang, et al.

Quansys Products Used:
Multiplex ELISA

Abstract:

Show Abstract (285 words)

Vaccine adjuvants are essential to drive a protective immune response in cases where vaccine antigens are weakly immunogenic, where vaccine antigen is limited, or where an increase in potency is needed for a specific population, such as the elderly. To discover novel vaccine adjuvants, we used a high-throughput screen (HTS) designed to identify small-molecule agonists of the RIG-I-like receptor (RLR) pathway leading to interferon regulatory factor 3 (IRF3) activation. RLRs are a group of cytosolic pattern-recognition receptors that are essential for the recognition of viral nucleic acids during infection. Upon binding of viral nucleic acid ligands, the RLRs become activated and signal to transcription factors, including IRF3, to initiate an innate immune transcriptional program to control virus infection. Among our HTS hits were a series of benzothiazole compounds from which we designed the lead analog, KIN1148. KIN1148 induced dose-dependent IRF3 nuclear translocation and specific activation of IRF3-responsive promoters. Prime-boost immunization of mice with a suboptimal dose of a monovalent pandemic influenza split virus H1N1 A/California/07/2009 vaccine plus KIN1148 protected against a lethal challenge with mouse-adapted influenza virus (A/California/04/2009) and induced an influenza virus-specific IL-10 and Th2 response by T cells derived from lung and lung-draining lymph nodes. Prime-boost immunization with vaccine plus KIN1148, but not prime immunization alone, induced antibodies capable of inhibiting influenza virus hemagglutinin and neutralizing viral infectivity. Nevertheless, a single immunization with vaccine plus KIN1148 provided increased protection over vaccine alone and reduced viral load in the lungs after challenge. These findings suggest that protection was at least partially mediated by a cellular immune component and that the induction of Th2 and immunoregulatory cytokines by a KIN1148-adjuvanted vaccine may be particularly beneficial for ameliorating the immunopathogenesis that is associated with influenza viruses.

Title:
Synthetic peptides for treatment of bacterial infections

Publication:
Google Patents 2017

Author(s):
Anat Shirvan, Dan Teleman, Gila Arad, et al.

Quansys Products Used:
16- Plex Multiplex Immunoassay

Abstract:

Show Abstract (21 words)

Title:
Characterization and purification of proteins suitable for the production of antibodies against ‘Ca. Liberibacter asiaticus

Publication:
Protein Expression and Purification, 2017: doi.org/10.1016/j.pep.2017.07.010

Author(s):
Huawei Liu, Sagheer Atta, John S. Hartung

Quansys Products Used:
Q-View Imager

Abstract:

Show Abstract (206 words)

Title:
Tumour-associated macrophages activate migration and STAT3 in pancreatic ductal adenocarcinoma cells in co-cultures

Publication:
Pancreatology 2017: doi.org/10.1016/j.pan.2017.04.013

Author(s):
Aino Salmiheimo, Harri Mustonen, Sanna Vainionpää, et al.

Quansys Products Used:
Human Q-Plex Custom Assay

Abstract:

Show Abstract (99 words)

Title:
Engagement of the Aryl Hydrocarbon Receptor in Mycobacterium tuberculosis–Infected Macrophages Has Pleiotropic Effects on Innate Immune Signaling

Publication:
Journal of Immunology, 2017: doi.org/10.4049/jimmunol.1501141

Author(s):
Babak Memari, Manuella Bouttier, Vassil Dimitrov, et al.

Quansys Products Used:
Sample Testing Service

Abstract:

Show Abstract (233 words)

Title:
Adiponectin/resistin interplay in serum and in adipose tissue of obese and normal-weight individuals

Publication:
Diabetology & Metabolic Syndrome, 2017: doi.org/10.1186/s13098-017-0293-2

Author(s):
Marta Izabela Jonas, Alina Kurylowicz, Zbigniew Bartoszewicz, et al.

Quansys Products Used:
Q-plex Custom array and Q-View Software

Abstract:

Show Abstract (99 words)

Title:
Targeting Aurora kinase A and JAK2 prevents GVHD while maintaining T reg and antitumor CTL function

Publication:
Science Translational Medicine, 2017: doi.org/10.1126/scitranslmed.aai8269

Author(s):
Brian C. Betts, Anandharaman Veerapathran, Joseph Pidala, et al.

Quansys Products Used:
Custom Multiplex Assay, Q-View Imager, and Q-View Software

Abstract:

Show Abstract (176 words)

Title:
Fast multiplex analysis of antibodies in complex sample matrix using the microfluidic Evalution™ platform

Publication:
Analytica Chimica Acta 2017: doi.org/10.1016/j.aca.2017.06.018

Author(s):
Karen Leirs, Pelin Leblebici, Jeroen Lammerty, et al.

Quansys Products Used:
Multiplex ELISA

Abstract:

Title:
Elderly Patient-Derived Endothelial Cells for Vascularization of Engineered Muscle

Publication:
Molecular Therapy 2017: doi.org/10.1016/j.ymthe.2017.02.011

Author(s):
Luba Perry, Moshe Y. Flugelman, Shulamit Levenberg

Quansys Products Used:
Custom-made multiplexed ELISA kit

Abstract:

Title:
5-Fluorouracil chemotherapy upregulates cytokines and alters hippocampal dendritic complexity in aged mice

Publication:
Behavioural Brain Research 2017: doi.org/10.1016/j.bbr.2016.08.039

Author(s):
Thomas R. Groves, Ryan Farris, Julie E. Anderson

Quansys Products Used:
Mouse Cytokine Array

Abstract:

Show Abstract (158 words)

Title:
Toxicoanthropology: Phthalate exposure in relation to market access in a remote forager-horticulturalist population

Publication:
International Journal of Hygiene and Environmental Health 2017: doi.org/10.1016/j.ijheh.2017.03.009

Author(s):
Marissa Sobolewskia, Bernard Weissa, Melanie Martinb, et al.

Quansys Products Used:
Multiplex Female Hormone Array/Q-View Imager

Abstract:

Show Abstract (307 words)

Phthalates are a class of plasticizing chemicals produced in high volume and widely found in consumer products. Evidence suggests that phthalates may have non-monotonic effects on reproductive hormone activity. With exposure to phthalates virtually ubiquitous among industrialized populations, identifying unexposed and/or minimally exposed human populations is essential for understanding the effects of low level exposures. Our primary objective was to quantify urinary phthalate metabolite concentrations in the Tsimane’, a remote population of Bolivian forager-horticulturalists. Our secondary objectives were to determine if phthalate metabolite concentrations vary in relation to access to market goods; and to explore relationships between phthalate and reproductive hormone metabolite concentrations. Given that phthalate exposure is of particular concern during fetal development, we focused on reproductive age women in the current analyses. Phthalate metabolites were assayed in urine samples from 59 naturally cycling, reproductive age Tsimane’ women. Market access was assessed as: (1) distance from residence to the largest nearby town (San Borja, Bolivia) and (2) Spanish fluency. Urinary reproductive hormone metabolite concentrations were quantified using enzyme immunoassays. We fit linear models to examine: (1) predictors of phthalate exposure; and (2) relationships between urinary phthalate and reproductive hormone metabolite concentrations. Eight phthalate metabolites were detectable in at least 75% of samples. Median concentrations were up to an order of magnitude lower than industrialized populations. Proximity to San Borja and Spanish fluency were strong predictors of exposure. In exploratory analyses, the sum of the di-2-ethylhexyl phthalate metabolites (∑DEHP) and Mono-isobutyl phthalate (MiBP) were significantly associated with altered concentrations of urinary reproductive hormone metabolites. Remote, subsistence populations, like the Tsimane’, offer a unique window into the health effects of endocrine active compounds because: (1) exposures are low and likely to be first generation; (2) a natural fertility lifestyle allows for exploration of reproductive effects; and (3) ever-increasing globalization will result in increasing exposure in the next decade.

Title:
Impact of tranexamic acid on coagulation and inflammation in murine models of traumatic brain injury and hemorrhage

Publication:
Journal of Surgical Research 2017: doi.org/10.1016/j.jss.2017.03.031

Author(s):
Ryan M. Boudreau, Mark Johnson, Rosalie Veile, et al.

Quansys Products Used:
16-Plex Q-Plex Mouse Cytokine Screen

Abstract:

Show Abstract (64 words)

Title:
Peptide Conjugated Particles

Publication:
Google Patents 2017

Author(s):
Lonnie D. Shea, Stephen D. Miller, Jonathan Woon Teck Yap, et al.

Quansys Products Used:
Multiplex ELISA and Q-View Software

Abstract:

Show Abstract (39 words)

Title:
Compositions including I-domain antigen conjugate compounds and methods for treatment of autoimmune disorders

Publication:
Google Patents 2017

Author(s):
Teruna J. Siahaan, Prakash Manikwar, Paul Kipkemboi Kiptoo, et al.

Quansys Products Used:
ELISA-based Q-Plex Mouse Cytokine-Screen

Abstract:

Show Abstract (78 words)

Title:
Variable Saline Concentrations for Initial Resuscitation Following Polytrauma

Publication:
University of Cincinnati, 2017

Author(s):
Dr. Michael Goodman

Quansys Products Used:
Q-Plex Multiplex Assay

Abstract:

Show Abstract (106 words)

Title:
VCAM-1 Mediates Cigarette Smoke Extract Enhancement of Monocyte Adhesion to Human Carotid Endothelial Cells

Publication:
Medical Research Archives, 2017

Author(s):
Gregory B. Pott, Mark Tsurudome, Jamie Bui, et al.

Quansys Products Used:
Q-Plex Multiplex ELISA

Abstract:

Show Abstract (135 words)

Title:
Perifosine Mechanisms of Action in Leishmania Species

Publication:
Antimicrobial Agents and Chemotherapy, 2017: doi.org/10.1128/AAC.02127-16.

Author(s):
Atteneri López-Arencibiaa, Carmen Martín-Navarro, Ines Sifaoui, et al.

Quansys Products Used:
Q-View Software

Abstract:

Show Abstract (61 words)

Title:
Bottom-up assembly of silicon nanowire conductometric sensors for the detection of apolipoprotein A1, a biomarker for bladder cancer

Publication:
Microchim Acta 2017: doi.org/10.1007/s00604-017-2288-y

Author(s):
Yen-Heng Lin, Wei-Siao Lin, Jing-Chao Wong, et al.

Quansys Products Used:
Q-Plex Multiplex Assay

Abstract:

Title:
α-1 Antitrypsin Promotes Semimature, IL-10–Producing and Readily Migrating Tolerogenic Dendritic Cells

Publication:
Journal of Immunology, 2017: doi.org/10.4049/jimmunol.1101340

Author(s):
Eyal Ozeri, Mark Mizrahi, Galit Shahaf, et al.

Quansys Products Used:
Q-Plex Mouse Cytokine and Q-View imager

Abstract:

Title:
Depression, evening salivary cortisol and inflammation in chronic fatigue syndrome: A psychoneuroendocrinological structural regression model

Title:
Multiplexed immunoassays: A new tool for better surveillance of vitamin and mineral deficiencies

Publication:
PATH, 2017

Author(s):
David Boyle

Quansys Products Used:
Human Micronutrient (Development)

Abstract:

Show Abstract (40 words)

Publication:
International Journal of Psychophysiology, 2017: doi.org/10.1016/j.ijpsycho.2017.09.009

Author(s):
Sara F.Milrad, Daniel L.Hall, Devika R.Jutagir, et al.

Quansys Products Used:
Custom Multiplex

Abstract:

Show Abstract (71 words)

Title:
Aged blood factors decrease cellular responses associated with delayed gingival wound repair

Publication:
PLOS One, 2017: doi.org/10.1371/journal.pone.0184189

Author(s):
María Paz Saldías, Mónica Cáceres, Patricio C. Smith, et al.

Quansys Products Used:
Multiplex ELISA

Abstract:

Show Abstract (351 words)

Aging is a gradual biological process characterized by a decrease in cell and organism functions. Gingival wound healing is one of the impaired processes found in old rats. Here, we studied the in vivo wound healing process using a gingival repair rat model and an in vitromodel using human gingival fibroblast for cellular responses associated to wound healing. To do that, we evaluated cell proliferation of both epithelial and connective tissue cells in gingival wounds and found decreased of Ki67 nuclear staining in old rats when compared to their young counterparts. We next evaluated cellular responses of primary gingival fibroblast obtained from young subjects in the presence human blood serum of individuals of different ages. Eighteen to sixty five years old masculine donors were classified into 3 groups: “young” from 18 to 22 years old, “middle-aged” from 30 to 48 years old and “aged” over 50 years old. Cell proliferation, measured through immunofluorescence for Ki67 and flow cytometry for DNA content, was decreased when middle-aged and aged serum was added to gingival fibroblast compared to young serum. Myofibroblastic differentiation, measured through alpha-smooth muscle actin (α-SMA), was stimulated with young but not middle-aged or aged serum both the protein levels and incorporation of α-SMA into actin stress fibers. High levels of PDGF, VEGF, IL-6R were detected in blood serum from young subjects when compared to middle-aged and aged donors. In addition, the pro-inflammatory cytokines MCP-1 and TNF were increased in the serum of aged donors. In old rat wound there is an increased of staining for TNF compared to young wound. Moreover, healthy gingiva (non injury) shows less staining compared to a wound site, suggesting a role in wound healing. Moreover, serum from middle-aged and aged donors was able to stimulate cellular senescence in young cells as determined by the expression of senescence associated beta-galactosidase and histone H2A.X phosphorylated at Ser139. Moreover, we detected an increased frequency of γ-H2A.X-positive cells in aged rat gingival tissues. The present study suggests that serum factors present in middle-aged and aged individuals may be responsible, at least in part, for the altered responses observed during wound healing in aging.

Title:
Toll-like receptor 4-dependent glial cell activation mediates the impairment in memory establishment induced by β-amyloid oligomers in an acute mouse model of Alzheimer’s disease

Publication:
Brain, Behavior, and Immunity 2017: doi.org/10.1016/j.bbi.2016.10.012

Author(s):
Claudia Balducci, Angelisa Frasca, Margherita Zotti, et al.

Quansys Products Used:
Q-Plex™ mouse cytokine array, image analysis software

Abstract:

Show Abstract (272 words)

Title:
In vitro evidence of efficacy and safety of a polymerized cat dander extract for allergen immunotherapy

Publication:
BMC Immunology 2017: doi.org/10.1186/s12865-017-0193-0

Author(s):
María Morales, Mayte Gallego, Victor Iraola, et al.

Quansys Products Used:
ELISA-based Q-Plex™

Abstract:

Show Abstract (53 words)

Title:
A double-blind, randomized trial on the effect of a broad-spectrum dietary supplement on key biomarkers of cellular aging including inflammation, oxidative stress, and DNA damage in healthy adults

Publication:
Journal of Clinical and Translational Research 2017: doi.org/10.18053/jctres.02.201604.004

Author(s):
Lucas C. Lages, Johanna Lopez, Ana Maria Lopez-Medrano, et al.

Quansys Products Used:
ELISA kit

Abstract:

Show Abstract (38 words)

Title:
Sensitive in vivo Visualization of Breast Cancer Using Ratiometric Protease-activatable Fluorescent Imaging Agent, AVB-620

Publication:
Theranostics, 2017: doi.org/10.7150/thno.20678

Author(s):
Marcel Miampamba, Junjie Liu, Alec Harootunian, et al.

Quansys Products Used:
Q-Plex Multiplex ELISA

Abstract:

Show Abstract (257 words)

Title:
Innovative encapsulation platform based on pancreatic extracellular matrix achieve substantial insulin delivery

Publication:
Journal of Controlled Release, 2017: doi.org/10.1016/j.jconrel.2016.07.045

Author(s):
D. Chaimov, L. Baruch, S. Krishtul, et al.

Quansys Products Used:
Q-Plex Multiplex Array

Abstract:

Show Abstract (138 words)

Title:
Simultaneous assessment of iodine, iron, vitamin A, malarial antigenemia, and inflammation status biomarkers via a multiplex immunoassay method on a population of pregnant women from Niger

Publication:
PLOS One, 2017: doi.org/10.1371/journal.pone.0185868

Author(s):
David Boyle

Quansys Products Used:
Human Micronutrient

Abstract:

Show Abstract (410 words)

Deficiencies of vitamin A, iron, and iodine are major public health concerns in many low- and middle-income countries, but information on their status in populations is often lacking due to high costs and logistical challenges associated with assessing micronutrient status. Accurate, user-friendly, and low-cost analytical tools are needed to allow large-scale population surveys on micronutrient status. We present the expansion of a 7-plex protein microarray tool for the simultaneous measurement of up to seven biomarkers with relevance to the assessment of the key micronutrients iron, iodine, and vitamin A, and inflammation and malaria biomarkers: α-1-acid glycoprotein, C-reactive protein, ferritin, retinol binding protein 4, soluble transferrin receptor, thyroglobulin, and histidine-rich protein II. Assay performance was assessed using international reference standards and then verified by comparing the multiplexed and conventional immunoassay results on a training panel of plasma samples collected from US adults. These data were used to assign nominal concentrations to the calibrators of the assay to further improve performance which was then assessed by interrogating plasma samples from a cohort of pregnant women from Niger. The correlation between assays for each biomarker measured from this cohort was typically good, with the exception of thyroglobulin, and the sensitivity ranged from 74% to 93%, and specificity from 81% to 98%. The 7-Plex micronutrient assay has the potential for use as an affordable tool for population surveillance of vitamin A, iron, and iodine deficiencies as well as falciparum malarial parasitemia infectivity and inflammation. The assay is easy-to-use, requires minimal sample volume, and is scalable, rapid, and accurateÐneeding only a low-cost reader and basic equipment present in most reference laboratory settings and so may be employed by low and middle income countries for micronutrient surveillance to inform on status in key populations. Micronutrient deficiencies including iron, iodine, and vitamin A affect a significant portion of the world’s population. Efforts to assess the prevalence of these deficiencies in vulnerable populations are challenging, partly due to measurement tools that are inadequate for assessing multiple micronutrients in large-scale population surveys. We have developed a 7-plex immunoassay for the simultaneous measurement of seven biomarkers relevant to assessing iodine, iron, and vitamin A status, inflammation and Plasmodium falciparum parasitemia by measuring levels of thyroglobulin, ferritin, soluble transferrin receptor, retinol binding protein 4, α-1-acid glycoprotein, C-reactive protein, and histidine-rich protein II. This 7-plex immunoassay technique has potential as a rapid and effective tool for use in large-scale surveys and assessments of nutrition intervention programs in low- and middle-income countries.

Title:
An elevated pro-inflammatory cytokines profile in Behcet’s disease: A multiplex analysis

Publication:
Immunology Letters, 2017: doi.org/10.1016/j.imlet.2016.12.001

Author(s):
Nasser Gholijani, Mohammad Reza Ataollahi, et al.

Quansys Products Used:
Cytokine Multiplex, Q-View Imager, and Q-View Software

Abstract:

Show Abstract (204 words)

Title:
Interleukin-1 receptor antagonist expression is inversely associated with outcomes of hepatitis B-related acute-on-chronic liver failure

Publication:
Experimental & Molecular Medicine 2017: doi.org/10.3892/etm.2017.4361

Author(s):
Jinglan Lai, Yuming Liu, Chen Pan, et al.

Quansys Products Used:
Q-Plex Multiplex Assay and Q-View Imager

Abstract:

Show Abstract (261 words)

Title:
Bromodomain-containing protein 2 induces insulin resistance via the mTOR/Akt signaling pathway and an inflammatory response in adipose tissue

Publication:
Cellular Signalling 2017: doi.org/10.1016/j.cellsig.2016.11.011

Author(s):
Ruixin Sun, Yi Wu, Weihua Hou, et al.

Quansys Products Used:
Singleplex Assay

Abstract:

Show Abstract (139 words)

Title:
New possible biomarkers for diagnosis of infections and diagnostic distinction between bacterial and viral infections in children

Publication:
Journal of Infection and Chemotherapy 2017: doi.org/10.1016/j.jiac.2016.11.002

Author(s):
Takashi Yusa, Kazuhiro Tateda, Akira Ohara, et al.

Quansys Products Used:
Q-Plex Cytokine Assay Kit

Abstract:

Show Abstract (173 words)

Title:
Paclitaxel-resistant cancer cell-derived secretomes elicit ABCB1-associated docetaxel cross-resistance and escape from apoptosis through FOXO3a-driven glycolytic regulation

Publication:
Experimental & Molecular Medicine 2017: doi.org/10.1038/emm.2016.131

Author(s):
Mark Borris D Aldonza, Ji-Young Hong, and Sang Kook Lee

Quansys Products Used:
16-plex Q-Plex Human Cytokine

Abstract:

Show Abstract (241 words)

Title:
Identification of multiple functional receptors for tyramine on an insect secretory epithelium

Publication:
Sci Rep 2017: doi.org/10.1038/s41598-017-00120-z

Author(s):
Haiying Zhang and Edward M. Blumenthal

Quansys Products Used:
Multiplex ELISA

Abstract:

Show Abstract (187 words)

Title:
Combinations of L-NG-monomethyl-arginine and oseltamivir against pandemic influenza A virus infections in mice

Publication:
Antiviral Chemistry and Chemotherapy 2017: doi.org/10.1177/2040206617691885

Author(s):
Donald F Smee, Ashley Dagley, EB Tarbet

Quansys Products Used:
Multiplex Array

Abstract:

Show Abstract (180 words)

Title:
Elevated angiopoietin 2 in aqueous of patients with neovascular age related macular degeneration correlates with disease severity at presentation

Publication:
Sci. Rep. 2017: doi.org/10.1038/srep45081

Author(s):
Danny S. Ng, Yolanda W. Yip, Malini Bakthavatsalam, et al.

Quansys Products Used:
Multiplex ELISA Array and Q-View Software

Abstract:

Show Abstract (167 words)

Title:
Performance of a High-Sensitivity Rapid Diagnostic Test for Plasmodium falciparum Malaria in Asymptomatic Individuals from Uganda and Myanmar and Naive Human Challenge Infections

Publication:
The American Journal of Tropical Medicine and Hygiene, 2017: doi.org/10.4269/ajtmh.17-0245

Author(s):
Smita Das, Ihn Kyung Jang, Becky Barney, et al.

Quansys Products Used:
Q-Plex ELISA Kit and Q-View Imager

Abstract:

Show Abstract (204 words)

Title:
Neuronal Inflammation: A Potential Contributing Mechanism to High Fat Diet-Induced Neuropathy

Publication:
Southern Illinois University, 2017

Author(s):
David S. Umbaugh

Quansys Products Used:
Sample Testing Service

Abstract:

Show Abstract (55 words)

Title:
An elevated pro-inflammatory cytokines profile in Behcet’s disease: A multiplex analysis

Publication:
Immunology Letters, 2017: doi.org/10.1016/j.imlet.2016.12.001

Author(s):
Nasser Gholijani, Mohammad Reza Ataollahi, Afshin Samiei, et al.

Quansys Products Used:
Human Cytokine 16-plex Assay

Abstract:

Title:
Different Regulation of Interleukin-1 Production and Activity in Monocytes and Macrophages: Innate Memory as an Endogenous Mechanism of IL-1 Inhibition

Publication:
Front Pharmacol, 2017: doi.org/10.3389/fphar.2017.00335

Author(s):
Mariusz P. Madej, Elfi Töpfer, Diana Boraschi, et al.

Quansys Products Used:
Custom Multiplex Assay

Abstract:

Show Abstract (307 words)

Production and activity of interleukin (IL)-1β are kept under strict control in our body, because of its powerful inflammation-promoting capacity. Control of IL-1β production and activity allows IL-1 to exert its defensive activities without causing extensive tissue damage. Monocytes are the major producers of IL-1β during inflammation, but they are also able to produce significant amounts of IL-1 inhibitors such as IL-1Ra and the soluble form of the decoy receptor IL-1R2, in an auto-regulatory feedback loop. Here, we investigated how innate immune memory could modulate production and activity of IL-1β by human primary monocytes and monocyte-derived tissue-like/deactivated macrophages in vitro. Cells were exposed to Gram-negative (Escherichia coli) and Gram-positive (Lactobacillus acidophilus) bacteria for 24 h, then allowed to rest, and then re-challenged with the same stimuli. The presence of biologically active IL-1β in cell supernatants was calculated as the ratio between free IL-1β (i.e., the cytokine that is not bound/inhibited by sIL-1R2) and its receptor antagonist IL-1Ra. As expected, we observed that the responsiveness of tissue-like/deactivated macrophages to bacterial stimuli was lower than that of monocytes. After resting and re-stimulation, a memory effect was evident for the production of inflammatory cytokines, whereas production of alarm signals (chemokines) was minimally affected. We observed a high variability in the innate memory response among individual donors. This is expected since innate memory largely depends on the previous history of exposure or infections, which is different in different subjects. Overall, innate memory appeared to limit the amount of active IL-1β produced by macrophages in response to a bacterial challenge, while enhancing the responsiveness of monocytes. The functional re-programming of mononuclear phagocytes through modulation of innate memory may provide innovative approaches in the management of inflammatory diseases, as well as in the design of new immunization strategies. In this respect, the interindividual variability in innate memory suggests the need of a personalized assessment.

Title:
Correlations of Kynurenic Acid, 3-Hydroxykynurenine, sIL-2R, IFN-α, and IL-4 with Clinical Symptoms During Acute Relapse of Schizophrenia

Publication:
Neurotox Res 2017: doi.org/10.1007/s12640-017-9714-0

Author(s):
Kinga SzymonaBarbara ZdzisińskaHanna Karakuła-Juchnowicz, et al.

Quansys Products Used:
Multiplex ELISA

Abstract:

Show Abstract (290 words)

Several lines of evidence suggest that up-regulation of immune response and alterations of kynurenine pathway function are involved in pathogenesis of schizophrenia. Correlations among clinical status (using PANNS, SANS and SAPS scales) and blood levels of kynurenic acid (KYNA), 3-hydroxykynurenine (3-HK) and levels of selected immunoactive molecules, soluble interleukin-2 receptor (sIL-2R), interferon-α (IFN-α) and IL-4 were analyzed in 51 chronic schizophrenia patients during acute relapse, after four weeks of therapy and at remission. KYNA levels were significantly lower in comparison with controls (N=45) throughout the study, whereas 3-HK did not differ from controls at admission and during therapy, but increased at remission. The KYNA/3-HK ratio and IL-4 levels, but not sIL-2R and IFN-α levels, were consistently decreased in schizophrenia patients at all analyzed time points. KYNA level and KYNA/3-HK ratio measured at admission correlated negatively with the duration of illness, whereas 3-HK level correlated negatively with the improvement of SANS score at discharge. sIL-2R level before treatment was positively linked with number of relapses. In the subgroup of patients with poor response to pharmacotherapy, treated with clozapine later on, initial KYNA level and the ratio KYNA/3-HK correlated negatively with number of relapses. Positive association of sIL-2R level with number of relapses was also evident in this subgroup. Furthermore, among these patients, starting IFN-α level was negatively linked with the improvement of total PANSS score at discharge. Presented here data support the concept of disturbed kynurenine pathway function in schizophrenia and suggest that assessment of KYNA and 3-HK levels during acute relapse might be useful in prediction of response to antipsychotic therapy. Deficit of peripheral KYNA and higher 3-HK levels could be associated with more severe symptoms of schizophrenia. Further studies with larger samples size are needed to validate our results.

Title:
A Novel Role for TL1A/DR3 in Protection against Intestinal Injury and Infection

Publication:
Journal of Immunology, 2017: doi.org/10.4049/jimmunol.1502466

Author(s):
Li-Guo Jia, Giorgos Bamias, Kristen O. Arseneau, et al.

Quansys Products Used:
Q-Plex 16-Plex Mouse Cytokine Screen

Abstract:

Show Abstract (199 words)

Title:
Selective Reduction of Brain Docosahexaenoic Acid after Experimental Brain Injury and Mitigation of Neuroinflammatory Outcomes with Dietary DHA

Publication:
Thieme Open Access, 2017: doi.org/ 10.1055/s-0037-1606836

Author(s):
Christopher M. Butt, Jordan L. Harrison, Rachel K. Rowe, et al.

Quansys Products Used:
Rat Cytokine Assay, Q-View Imager Pro and Q-View Software

Abstract:

Show Abstract (124 words)

Title:
Long-Term Feeding With Bioactive Tripeptides in Aged Hypertensice and Normotensive Rats: Special Focus on Blood

Publication:
Journal of Pharmacy and Pharmacology, 2017

Author(s):
A. Siltari, J. Roivanen, R. Korpela, et al.

Quansys Products Used:
Rat Cytokine Array

Abstract:

Show Abstract (306 words)

Bradykinin is the main player of the kallikrein-kinin system. Bradykinin-induced vasodilatation is age-dependent; this is believed to be associated with the level of expression of the two bradykinin receptors (BR1 and BR2) in the vasculature. The aim of this study was to clarify bradykinin-induced vascular reactivity of spontaneously hypertensive rats (SHR) and age-matched Wistar-Kyoto rats (WKY) after 6 weeks’ consumption of a drink containing bioactive tripeptides (Ile-Pro-Pro, Val-Pro-Pro and Leu-Pro-Pro). Two age groups were used: young (10 weeks at the end of experiment) and old (24 weeks). Blood pressure was monitored weekly by the tail-cuff method. After six weeks, vascular reactivity was assessed in vitro in mesenteric artery rings focusing on bradykinin induced activity. Blood pressure was lowered in old SHR after 6 weeks’ tripeptide consumption compared to water drinking controls (P < 0.05). Blood pressure was lowered by peptide consumption also in old WKY (P < 0.05) but tripeptide consumption exerted no effect on the blood pressure of young animals. Old SHR suffered from endothelial and smooth muscle dysfunction which was not improved by these tripeptides. Interestingly, bradykinin caused vasoconstriction even in young SHR; this was blocked by a non-selective cyclooxygenase (COX) inhibitor but not by a B1 and B2 receptor antagonist. The expressions of mRNA of COX-1 and COX-2 in aorta were slightly upregulated in old SHR. ACE-1 activity in aorta and protein level in kidney, but not ACE-1 mRNA expression was upregulated in old animals (P < 0.05). To conclude, long-term feeding with a drink containing tripeptides lowers or prevents the age-associated increase in blood pressure in hypertensive and normotensive animals. ACE-1 activity, protein level but not mRNA expression are elevated in old animals. We also demonstrated that the vascular inflammation and dysfunction present in aged hypertensive animals cause bradykinin to induce vasoconstriction; this is not prevented by tripeptide feeding but involves the prostaglandin pathway.

Title:
Relationship between family history of type 2 diabetes and serum FGF21

Publication:
European Journal of Clinical Investigation 2017: doi.org/10.1111/eci.12835

Author(s):
Greggory R. Davis, Tiffany Deville, Joshua Guillory, et al.

Quansys Products Used:
Multiplex Assay Kit and Q-View Imager

Abstract:

Show Abstract (100 words)

2016 Publications

Title:
Experimental Support for the Ecoimmunity Theory: Distinct Phenotypes of NonLymphocytic Cells in SCID and Wild Type Mice

Publication:
Cell Transplantation 2016: doi.org/10.3727/096368916X690809

Author(s):
David E. Ochayon, Boris M. Baranovski, Peter Malkin

Quansys Products Used:
Q-Plex mouse cytokine chemiluminescence-based 9-plex ELISA

Abstract:

Show Abstract (300 words)

Immune tolerance towards ‘self’ is critical in multiple immune disorders. While there are several mechanisms to describe the involvement of immune cells in the process, the role of peripheral tissue cells in that context is not yet clear. The theory of Ecoimmunity postulates that interactions between immune and tissue cells represent a predator-prey relationship. This life-long interaction leads to selection of non-immune cell phenotypes during ontogeny. Normally, therefore, nonimmune cells that evolve alongside an intact immune system would be phenotypically capable of evading immune responses, and cells whose phenotype falls short of satisfying this steady-state would expire under hostile immune responses. This view was supported, until recently, by experimental evidence depicting an inferior endurance of SCID-derived pancreatic islets upon engraftment into syngeneic immune-intact syngeneic WT mice. In the present study, we challenge several phenotypic changes suggested by the Ecoimmunity theory. Immune-related phenotypes of islets, spleen and bone marrow immune cells were determined, as well as SCID and WT non-lymphocytic cells. Islet submass grafting was performed in order to depict syngeneic graft functionality. Islet cultures were examined under both resting and inflamed conditions for expression of CD40 and MHC class I/II, and release of IL-1, IL-1, IL-6, TNF, IL-10 and insulin. Results depict multiple pathways that appear to be related to the sculpting of non-immune cells by immune cells; 59 SCID islet genes displayed relative expression changes
compared to WT islets; SCID cells expressed lower tolerability to inflammation and greater levels of immune-related molecules, including MHC class I. Accordingly, islets exhibited a marked increase in insulin release upon immunocyte depletion, in effect resuming endocrine function that was otherwise suppressed by resident immunocytes. This work provides further support to the Ecoimmunity theory and encourages subsequent studies to identify its role in the emergence and treatment of autoimmune pathologies, transplant rejection and cancer.

Title:
Concentration and ratio of essential fatty acids influences the inflammatory response in lipopolysaccharide challenged mice

Publication:
Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA) 2016: http://dx.doi.org/10.1016/ j.plefa.2016.03.003

Author(s):
K.J. Hintze, J. Tawzera, R.E. Warda

Quansys Products Used:
Q-Plex™ Mouse Cytokine – Screen (16-plex) array

Abstract:

Show Abstract (163 words)

Title:
Intranasal delivery of a protein subunit vaccine using a Tobacco Mosaic Virus platform protects against pneumonic plague

Publication:
Vaccine 2016: http://dx.doi.org/10.1016/ j.vaccine.2016.09.063

Author(s):
Paul M. Arnaboldi, Mariya Sambir, Christina D’Arco, et al.

Quansys Products Used:
Quansys Multiplex ELISA and Quansys Plate Reader Software

Abstract:

Title:
Continuous exposure to non-lethal doses of sodium iodate induces retinal pigment epithelial cell dysfunction

Publication:
Sci Rep. 2016: doi.org/10.1038/srep37279

Author(s):
Xiao-Yu Zhang, Tsz Kin Ng, Marten Erik Brelen, et al.

Quansys Products Used:
Multiplex ELISA Array and Q-View Software

Abstract:

Show Abstract (196 words)

Title:
Apoptotic protein profile in Leishmania donovani after treatment with hexaazatrinaphthylenes derivatives

Publication:
Experimental Parasitology. (2016) doi.org/10.1016/j.exppara.2016.04.005

Author(s):
Atteneri López-Arencibiaa, Carmen M Martín-Navarroa, Ines Sifaoui, et al.

Quansys Products Used:
Q-View Software

Abstract:

Title:
Illness progression in chronic fatigue syndrome: a shifting immune baseline

Publication:
BMC Immunology201617:3 doi.org/10.1186/s12865-016-0142-3

Author(s):
Lindsey Russell, Gordon Broderick, Renee Taylor, et al.

Quansys Products Used:
Human Cytokine-Screen (16-plex), Q-View Imager, Q-View Software

Abstract:

Title:
Cytokines in cerebrospinal fluid of neurosyphilis patients: Identification of Urokinase plasminogen activator using antibody microarrays

Publication:
J Neuroimmunology. (2016) doi.org/10.1016/j.jneuroim.2015.12.010

Author(s):
Ping Lua, Dao-Cheng Zhenga, Chang Fang, et al.

Quansys Products Used:
Q-View Imager, Q-View Software

Abstract:

Title:
Calcitonin gene-related peptide contributes to peripheral nerve injury-induced mechanical hypersensitivity through CCL5 and p38 pathways

Publication:
J Neuroimmunology, (2016) doi.org/10.1016/j.jneuroim.2016.05.003

Author(s):
Jennifer T. Malon, Ling Cao

Quansys Products Used:
Sample Testing Service

Abstract:

Show Abstract (97 words)

Title:
Correspondence of Neutralizing Humoral Immunity and CD4 T Cell Responses in Long Recovered Sudan Virus Survivors

Publication:
Viruses 2016, 8(5), 133; doi.org/10.3390/v8050133

Author(s):
Ariel Sobarzo, Spencer W. Stonier, Andrew S. Herbert, et al.

Quansys Products Used:
Custom Human Q-Plex Array, Q-View Imager, Q-View Software

Abstract:

Show Abstract (140 words)

Title:
A Novel Role for TL1A/DR3 in Protection against Intestinal Injury and Infection

Publication:
J Immunol. 2016: doi.org/10.4049/jimmunol.1502466

Author(s):
Li-Guo Jia, Giorgos Bamias, Kristen O. Arseneau, et al.

Quansys Products Used:
Q-Plex Array Mouse Cytokine Screen (16-plex)

Abstract:

Show Abstract (199 words)

Title:
Activation of RNase L by Murine Coronavirus in Myeloid Cells Is Dependent on Basal Oas Gene Expression and Independent of Virus-Induced Interferon

Publication:
Journal of Virology. 2016: doi.org/10.1128/JVI.03036-15

Author(s):
L. Dillon Birdwell, Zachary B. Zalinger, Yize Li, et al.

Quansys Products Used:
100 U/ml universal IFN-α

Abstract:

Show Abstract (223 words)

Title:
Derivation of hematopoietic cells from adult mesenchymal stem cells

Publication:
Google Patents. 2016

Author(s):
Reza Izadpanah and Eckhard Alt

Quansys Products Used:
ELISA Kits and Quansys Q-View™ Software

Abstract:

Show Abstract (24 words)

Title:
Development of Electrochemical Immunosensors towards Point-of-care Cancer Diagnostics: Clinically Relevant Studies

Publication:
Electroanalysis. 2016: doi.org/10.1002/elan.201600248

Author(s):
Rawiwan Laocharoensuk

Quansys Products Used:
Q-Plex Multiplex ELISA

Abstract:

Show Abstract (130 words)

Title:
An elevated pro-inflammatory cytokines profile in Behcet’s disease: A multiplex analysis

Publication:
Immunology Letters. 2016: doi.org/10.1016/ j.imlet.2016.12.001

Author(s):
Nasser Gholijani, Mohammad Reza Ataollahi, Afshin Samiei, et al.

Quansys Products Used:
16-Plex Multiplex Human Cytokine Assay

Abstract:

Show Abstract (204 words)

Title:
Preparation of Primary Mixed Glial Cultures from Adult Mouse Spinal Cord Tissue

Publication:
Department of Biomedical Sciences. 2016: doi.org/10.3791/54801

Author(s):
Jennifer T. Malon, Ling Cao

Quansys Products Used:
Mouse Chemokine Q-Plex

Abstract:

Show Abstract (252 words)

Title:
A combination of isolated phytochemicals and botanical extracts lowers diastolic blood pressure in a randomized controlled trial of hypertensive subjects

Publication:
European Journal of Clinical Nutrition. 2016: doi.org/ 10.1038/ejcn.2015.88

Author(s):
S Biesinger, H A Michaels, A S Quadros, et al.

Quansys Products Used:
Multiplex Assay

Abstract:

Show Abstract (111 words)

Title:
Role of erythropoietin in the angiogenic activity of bone marrow endothelial cells of MGUS and multiple myeloma patients

Publication:
Oncotarget 2016: doi.org/10.18632/oncotarget.7587

Author(s):
Aurelia Lamanuzzi, Ilaria Saltarella, Arianna Ferrucci

Quansys Products Used:
Q-Plex™ Array Human Angiogenesis Antigen

Abstract:

Show Abstract (185 words)

Title:
Exploration of α1-Antitrypsin Treatment Protocol for Islet Transplantation: Dosing Plan and Route of Administration

Publication:
Journal of Pharmacology and Experimental Therapeutics 2016: https://doi.org/10.1124/ jpet.116.236067

Author(s):
Boris M. Baranovski, Eyal Ozeri, Galit Shahaf, et al.

Quansys Products Used:
Q-Plex 8-Plex Mouse Cytokine

Abstract:

Show Abstract (182 words)

Title:
Hypothetical Link Between Osteopathic Suboccipital Decompression and Neuroimmunomodulation

Publication:
Journal of Neurology and Neuroscience 2016

Author(s):
Joshua A Cuoco, Charles N Fennie, George K Cheriyan

Quansys Products Used:
Human Cytokine – Stripwells (16-plex) ELISA

Abstract:

Title:
Induction of histone deacetylases (HDACs) in human abdominal aortic aneurysm: therapeutic potential of HDAC inhibitors

Publication:
Disease Models & Mechanisms 2016 9: 541-552; doi.org/10.1242/dmm.024513

Author(s):
María Galán, Saray Varona, Mar Orriols, et al.

Quansys Products Used:
Mouse Cytokine Array

Abstract:

Show Abstract (248 words)

Title:
Amelioration of Chikungunya through Inhibition of the Inflammatory Response

Publication:
All Graduate Theses and Dissertations. Paper 4996.

Author(s):
Ashley L. Dagley

Quansys Products Used:
Mouse Cytokine Array (16-plex), Q-View Imager, Q-View Software

Abstract:

Title:
Effects of Telephone-Delivered Cognitive Behavioral Stress Management Intervention on Fatigue Interference and Neuroimmune Function in Chronic Fatigue Syndrome

Publication:
University of Miami Scholarly Repository, Open Access Dissertations

Author(s):
Daniel L. Hall

Quansys Products Used:
Human Cytokine Screen, Q-View Imager, Q-View Software

Abstract:

Show Abstract (511 words)

The perceived impact of chronic fatigue on daily living (i.e., fatigue interference) is particularly relevant for patients diagnosed with Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME), a medically unexplained illness associated with neuroendocrine and immune abnormalities. Literature suggests that fatigue interference is higher among women with CFS/ME than with other women facing chronic fatigue concerns, such as cancer survivors. To date, these comparisons have been primarily qualitative, limiting the ability to statistically control for related factors such as fatigue severity. Furthermore, greater fatigue interference in CFS/ME may relate to a suppressed cortisol awakening response (CAR) and heightened levels of the pro-inflammatory cytokine interleukin-6 (IL-6), though these associations have not been tested before. Finally, previous cognitive behavioral interventions including cognitive behavioral stress management (CBSM) have been shown to be helpful for this population, leading to improvements in psychological functioning and less dysregulated physiology. Given the high degree of fatigue and debilitating symptoms in CFS/ME, the efficacy of a 10 session, telephone-delivered CBSM intervention on fatigue interference and neuroimmune function over time was investigated. In Study 1, previously collected data on fatigue interference and fatigue severity were examined among 95 women with CFS/ME and 67 fatigued breast cancer survivors approximately 5 years post treatment. Analyses controlled for age, race/ethnicity, education level, marital status, employment status, number of children, time since diagnosis, and fatigue severity. Women with CFS/ME were found to endorse higher fatigue interference scores, p < .001. Next, neuroimmune correlates to fatigue interference scores were assessed among the CFS/ME sample. Again controlling for relevant covariates, higher fatigue interference scores were associated with a more diminished CAR with respect to increase (CARi), p = .02. No relationships were observed between fatigue interference and the CAR with respect to ground (CARg) or IL-6 levels. Additionally, these relationships were not amplified in the presence of high depressed mood. In Study 2, the effects of a 10-session, telephone-delivered cognitive behavioral stress management (CBSM) intervention on these variables were assessed. Participants included 93 women with CFS/ME from Study 1 who were randomized to either the CBSM (n = 53) or attention-matched control condition (n = 40). Results failed to identify intervention effects on changes in these variables from baseline (BL) to five months (5M) or nine months (9M) later. This may have been due to comparisons with a strong control condition, or to potential limitations in participants’ engagement via telephone. Interestingly, the CARi, CARg, post-awakening cortisol, and IL-6 were observed to decrease significantly over time in both conditions (ps < .05). Mechanisms of change might include gains in self-efficacy due to mastery of skills in either CBSM or attention control conditions. Examination of potential lagged effects of CBSM on cortisol and IL-6 levels warrants future investigation, as lowest levels of these biomarkers were at 9M. Future studies could also use videophone delivery of CBSM, which might bolster participant engagement in sessions and help to reach homebound or highly symptomatic CFS/ME patients. Emerging biomarkers of neuroimmune dysfunction in this population may yield insights into mechanisms underlying this elusive illness and help to identify new targets for psychosocial approaches to care.

Title:
Effect of Acer tegmentosum bark on atopic dermatitis-like skin lesions in NC/Nga mice

Publication:
J Ethnopharmacology. (2016) doi.org/10.1016/j.jep.2015.10.033

Author(s):
Gabsik Yanga, Duckgun Ana, Mi-Hwa Leea, et al.

Quansys Products Used:
Mouse Cytokine-Screen (16-plex) and Q-View Imager Pro

Abstract:

Title:
Introducing MARCo: Histoserological Findings of a Multi-Organic Paraneoplastic Syndrome in Cutaneous Melanoma Patients

Publication:
Dermatology and Therapy. (2016) doi.org/10.1007/s13555-016-0142-5

Author(s):
Ranjbar, M., Mohi, A., Pföhler, C. et al.

Quansys Products Used:
Human Cytokine Array, Q-View Software

Abstract:

Show Abstract (95 words)

Title:
Cannabinoid Receptor-Specific Mechanisms To Alleviate Pain In Sickle Cell Anemia Via Inhibition Of Mast Cell Activation And Neurogenic Inflammation

Publication:
Haematologica. 2016: doi.org/10.3324/ haematol.2015.136523

Author(s):
Lucile Vincent, Derek Vang, Julia Nguyen, et al.

Quansys Products Used:
Q-Plex™ Array

Abstract:

Show Abstract (151 words)

Title:
Comparison of AS03 and Alum on immune responses elicited by A/H3N2 split influenza vaccine in young, mature and aged BALB/c mice

Publication:
Vaccine. 2016: doi.org/10.1016/ j.vaccine.2016.02.012

Author(s):
Karen K. Yama, Jyotsana Guptaa, Elizabeth K. Allena, et al.

Quansys Products Used:
Q-Plex Mouse Cytokine assay (16-plex)

Abstract:

Show Abstract (82 words)

Title:
Development and in vivo safety assessment of tenofovir-loaded nanoparticles-in-film as a novel vaginal microbicide delivery system

Publication:
Acta Biomaterialia. 2016: doi.org/10.1016/ j.actbio.2016.08.018

Author(s):
Alexandra Machadoa, Cassilda Cunha-Reisc, Francisca Araújoa, et al.

Quansys Products Used:
Q-Plex™ Cytokine Array

Abstract:

Show Abstract (312 words)

Topical pre-exposure prophylaxis (PrEP) with antiretroviral drugs holds promise in preventing vaginal transmission of HIV. However, significant biomedical and social issues found in multiple past clinical trials still need to be addressed in order to optimize protection and users’ adherence. One approach may be the development of improved microbicide products. A novel delivery platform comprising drug-loaded nanoparticles (NPs) incorporated into a thin polymeric film base (NPs-in-film) was developed in order to allow the vaginal administration of the microbicide drug candidate tenofovir. The system was optimized for relevant physicochemical features and characterized for biological properties, namely cytotoxicity and safety in a mouse model. Tenofovir-loaded poly(lactic-co-glycolic acid) (PLGA)/stearylamine (SA) composite NPs with mean diameter of 127 nm were obtained with drug association efficiency above 50%, and further incorporated into an approximately 115 μm thick, hydroxypropyl methylcellulose/poly(vinyl alcohol)-based film. The system was shown to possess suitable mechanical properties for vaginal administration and to quickly disintegrate in approximately 9 min upon contact with a simulated vaginal fluid (SVF). The original osmolarity and pH of SVF was not affected by the film. Tenofovir was also released in a biphasic fashion (around 30% of the drug in 15 min, followed by sustained release up to 24 h). The incorporation of NPs further improved the adhesive potential of the film to ex vivo pig vaginal mucosa. Cytotoxicity of NPs and film was significantly increased by the incorporation of SA, but remained at levels considered tolerable for vaginal delivery of tenofovir. Moreover, histological analysis of genital tissues and cytokine/chemokine levels in vaginal lavages upon 14 days of daily vaginal administration to mice confirmed that tenofovir-loaded NPs-in-film was safe and did not induce any apparent histological changes or pro-inflammatory response. Overall, obtained data support that the proposed delivery system combining the use of polymeric NPs and a film base may constitute an exciting alternative for the vaginal administration of microbicide drugs in the context of topical PrEP.

Title:
Macrophage Cells Secrete Specific Cytokines and Accumulate Activated Interferon Regulatory Factor 3 after Multi-Walled Carbon Nanotube Exposure

Publication:
J Nanomater Mol Nanotechnol. 2016: doi.org/10.4172/ 2324-8777.1000195

Author(s):
Kevin D Houston, Nathan H Mack, Stephen K Doorn, et al.

Quansys Products Used:
Q-Plex Mouse Cytokine Array

Abstract:

Show Abstract (171 words)

Title:
Elevated ccl19 after completion of therapy for acute lyme disease identifies patients at risk for development of post-treatment lyme disease syndrome who will benefit from further antibiotic therapy

Publication:
The Johns Hopkins University. 2016

Author(s):
John N. Aucott, Mark J. Soloski

Quansys Products Used:
Multiplex ELISA

Abstract:

Title:
Acquired resistance of pancreatic cancer cells to treatment with gemcitabine and HER-inhibitors is accompanied by increased sensitivity to STAT3 inhibition

Publication:
International Journal of Oncology. 2016: doi.org/ 10.3892/ijo.2016.3320

Author(s):
Nikolaos Ioannou, Alan M. Seddon, Angus Dalgleish, et al.

Quansys Products Used:
Q-View Software

Abstract:

Show Abstract (348 words)

Drug-resistance is a major contributing factor for the poor prognosis in patients with pancreatic cancer. We have shown previously that the irreversible ErbB family blocker afatinib, is more effective than the reversible EGFR tyrosine kinase inhibitor erlotinib in inhibiting the growth of human pancreatic cancer cells. The aim of this study was to develop human pancreatic cancer cell (BxPc3) variants with acquired resistance to treatment with gemcitabine, afatinib, or erlotinib, and to investigate the molecular changes that accompany the acquisition of a drug-resistant phenotype. We also investigated the therapeutic potential of various agents in the treatment of such drug-resistant variants. Three variant forms of BxPc3 cells with acquired resistance to gemcitabine (BxPc3GEM), afatinib (BxPc3AFR) or erlotinib (BxPc3OSIR) were developed following treatment with increasing doses of such drugs. The expression level, mutational and phosphorylation status of various growth factor receptors and downstream cell signaling molecules were determined by FACS, human phopsho-RTK array, and western blot analysis while the sulforhodamine B assay was used for determining the effect of various agents on the growth of such tumours. We found that all three BxPc3 variants with acquired resistance to gemcitabine (BxPc3GEM), afatinib (BxPc3AFR) or erlotinib (BxPc3OSIR) also become less sensitive to treatment with the two other agents. Acquisition of resistance to these agents was accompanied by upregulation of p-c-MET, p-STAT3, CD44, increased autocrine production of EGFR ligand amphiregulin and differential activation status of EGFR tyrosine residues as well as downregulation of total and p-SRC. Of all therapeutic interventions examined, including the addition of an anti-EGFR antibody ICR62, an anti-CD44 monoclonal antibody, and of STAT3 or c-MET inhibitors, only treatment with the STAT3 inhibitor Stattic produced a higher growth inhibitory effect in all three drug-resistant variants. In addition, treatment with a combination of afatinib with either c-MET inhibitor Crizotinib or Stattic resulted in an additive or synergistic growth inhibition in all three variants. Our results suggest that activation of STAT3 may play an important role in the acquisition of resistance to gemcitabine and HER inhibitors in pancreatic cancer and warrant further studies on the therapeutic potential of STAT3 inhibitors in such a setting.

Title:
Innovative encapsulation platform based on pancreatic extracellular matrix achieve substantial insulin delivery

Publication:
Journal of Controlled Release. 2016: doi.org/10.1016/ j.jconrel.2016.07.045

Author(s):
D. Chaimov, L. Barucha, S. Krishtul, I. Meivar-levy, et al.

Quansys Products Used:
Q-Plex Array Chemiluminescent Kit

Abstract:

Show Abstract (138 words)

Title:
Effect of Vitamin D on Peripheral Blood Mononuclear Cells from Patients with Psoriasis Vulgaris and Psoriatic Arthritis

Publication:
Department of Dermatology. 2016: doi.org/10.1371/ journal.pone.0153094

Author(s):
Susana Cubillos , Nadine Krieg, Johannes Norgauer

Quansys Products Used:
Q-View Software

Abstract:

Show Abstract (104 words)

Title:
Abstract B057: The CD122-biased immunostimulatory cytokine NKTR-214 combined with checkpoint blockade leads to mobilization of antitumor immunity and synergistic activity

Publication:
Second CRI-CIMT-EATI-AACR International Cancer Immunotherapy Conference 2016: doi.org/10.1158/2326-6066.IMM2016-B057

Author(s):
John L. Langowski, Murali Addepalli, Laurie VanderVeen, et al.

Quansys Products Used:
Quansys Multiplex ELISA

Abstract:

Show Abstract (541 words)

Background: NKTR-214 is a novel immunostimulatory cytokine which provides a sustained and biased activation signal through the heterodimeric IL-2R receptor (IL2Rbg or CD122), resulting in significant expansion of CD8+ T and NK cells relative to regulatory T cells in the tumor microenvironment. As a single agent in preclinical tumor models, NKTR-214 significantly increases the intratumoral CD8T/Treg ratio (>400). Here we examine activity and pharmacodynamic effects of NKTR-214 alone and combined with immune checkpoint blockade in both a murine tumor model and in a human in vitro co-culture system. Methods: Mice bearing subcutaneous CT26 colon tumors were treated with single agent NKTR-214 (q9d), murine anti-CTLA-4 or anti-PD-1 (twice-weekly), or combinations of these agents. Serum cytokines were assessed by multiplex ELISA (Quansys). T cell clonality and infiltration were assessed in tumors 7 days after treatment initiation, with TCR V and J beta usage determined by ImmunoSEQ (Adaptive Biotechnologies). Effects on human cytokine and chemokine expression were examined by the BioMAP Combo ELECT (DiscoverX) in vitro system utilizing human fibroblasts or endothelial cells, PBMCs and either HT29 colon adenocarcinoma or HT1299 NSCLC cell lines. 1-PEG-IL2, the most active species of the NKTR-214 prodrug, or Keytruda (anti-PD-1) was added over a range of concentrations alone or in combination, with biomarkers assessed 48 hours later. Results: In tumor-bearing mice, NKTR-214 led to increases in serum IFNg as well as increases in the chemokine MCP-1 (CCL2), a chemoattractant of CD4 T, CD8 T and NK cells. Single-agent NKTR-214 led to significant tumor growth inhibition and 10% tumor free animals, while single-agents anti-CTLA-4 or anti-PD-1 lacked efficacy. Combination of NKTR-214 with checkpoint blockade was synergistic and led to greater tumor-free animals (67% in combination with anti-CTLA-4, 90% with anti-PD-1). NKTR-214 combined with anti-PD-1 was also more efficacious than the combination of anti-CTLA-4 and anti-PD-1. TCR repertoire analysis demonstrated superior increases in TIL clonality and infiltration with NKTR-214 compared to either anti-CTLA-4 or anti-PD-1 alone. NKTR-214 combined with either mode of checkpoint inhibition led to significant increases in both parameters, with the greatest effect occurring when combined with anti-PD-1. In the human BioMAP system, NKTR-214 active species led to significant increases in granzyme B, IFNg, IL-6, IL-17A and TNFa, while these markers were only modestly induced by Keytruda alone. When NKTR-214 was combined with Keytruda, additive and synergistic increases in these factors were found. In addition, the combination significantly decreased uPA in the fibroblast-HT29 system and MDC in the endothelial-HT1299 system, both factors associated with tumor progression. Conclusions: NKTR-214 delivers sustained signaling of the IL-2 pathway. Analysis of multiple immune markers in mice and human tumor-immune cell cultures provide mechanistic rationale for the increased T and NK cell activation in the tumor microenvironment after treatment with NKTR-214. When combined with anti-PD-1, significant increases in T cell receptor clonality, tumor infiltration and immune activation biomarkers are achieved – a significant finding, given that concomitant increase in clonality and infiltration has been correlated with favorable responses in the clinic. In vitro studies with human cells demonstrate the potentiation of immune activation markers associated with anti-tumor effects in combination with anti-PD-1, further suggesting these findings may be translatable from mouse to human. NKTR-214 is currently in a Phase 1 clinical trial to evaluate the pharmacokinetics, pharmacodynamics and activity in an outpatient setting.

Title:
Simultaneous capture and sequential detection of two malarial biomarkers on magnetic microparticles

Publication:
Talanta 2016: http://dx.doi.org/10.1016/ j.talanta.2016.08.078

Author(s):
Christine F. Markwalter, Keersten M. Ricks, Anna L. Bitting, et al.

Quansys Products Used:
Quansys Multiplex ELISA

Abstract:

Show Abstract (167 words)

Title:
Stromal CCR6 drives tumor growth in a murine transplantable colon cancer through recruitment of tumor-promoting macrophages

Publication:
OncoImmunology 2016: doi.org/10.1080/ 2162402X.2016.1189052

Author(s):
Bisweswar Nandi, Mia Shapiro, Mehmet K. Samur, et al

Quansys Products Used:
Q-Plex Chemiluminescent Kit and Q-View Software

Abstract:

Title:
vIL-10-overexpressing human MSCs modulate naïve and activated T lymphocytes following induction of collagenase-induced osteoarthritis

Title:
Impaired RASGRF1/ERK–mediated GM-CSF response characterizes CARD9 deficiency in French-Canadians

Publication:
J Allergy and Clinical Immunology. (2016) doi.org/10.1016/ j.jaci.2015.09.016

Author(s):
Christina Gavino, Nancy Hamel, Ji Bin Zeng, et al.

Quansys Products Used:
Human Cytokine Array

Abstract:

Show Abstract (64 words)

Publication:
Stem Cell Research & Therapy20167:74 doi.org/10.1186/s13287-016-0331-2

Author(s):
Eric Farrell, Niamh Fahy, Aideen E Ryan, et al.

Quansys Products Used:
Mouse Cytokine-Screen (16-plex) and Q-View Software

Abstract:

Show Abstract (117 words)

Title:
Poor Sleep Quality, Circulating Pro-Inflammatory Cytokines and Severity and Frequency of Chronic Fatigue Syndrome/ Myalgic Encephalomyelitis (CFS/ME) Symptoms in Women

Publication:
University of Miami Scholarly Repository, Open Access Theses

Author(s):
Sara Milrad

Quansys Products Used:
Human Cytokine Screen, Q-View Imager, Q-View Software

Abstract:

Show Abstract (644 words)

Chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME) is a debilitating illness that is over represented among women and characterized by extreme fatigue and myriad distressing somatic complaints, with no known etiology or putative biomarker, though recent research has shown evidence of immunological abnormalities in CFS/ME. One of the major symptoms of CFS/ME is experiencing unrefreshing sleep or poor subjective sleep quality, which as a construct, can encompass sleep duration, sleep efficiency, sleep latency, and sleep disturbances, among other aspects of sleep quality. The subjective account of poor sleep quality has been consistently shown in CFS/ME, though objective evidence of sleep-related abnormalities by polysomnography or multiple sleep latency tests are inconsistent. Despite evidence of increased fatigue, somatic symptoms, pro-inflammatory cytokines and poor subjective sleep quality in CFS/ME, the association among these variables has not yet been studied extensively in women with CFS/ME, even though the relationship between poor subjective and objective sleep quality and inflammation, fatigue, and symptom severity has been established in other healthy and chronically ill populations. Additionally, CFS/ME patients report increased prevalence of mood disorders such as depression. Depression has been linked to both inflammation and poor sleep quality in other contexts, but not yet in CFS/ME. Due to depression’s known relationship to increased inflammation and poor sleep quality, I hypothesized that the relationship between poor sleep quality and inflammation would be more salient for more depressed CFS/ME women. This study sought to examine the main effects of poor sleep quality on pro and anti-inflammatory cytokines, fatigue severity and interference, symptom severity and frequency in CFS/ME women and also measure the moderating effect of depression on the relationship between poor sleep quality and pro and anti-inflammatory cytokines. In total, 95 women with CFS/ME provided blood samples, as well as self-reported measures of poor sleep quality, depressive symptomatology, CFS/ME somatic symptoms and fatigue. On average patients scored in the clinically elevated range for poor sleep quality on the Pittsburgh Sleep Quality Index (PSQI) global score. Multiple regression analyses showed that worse sleep quality overall (PSQI global score) related to more severe and more frequent CFS (non-sleep) symptoms, more fatigue severity and interference in daily life, and greater levels of inflammation (interleukin-2 [IL-2], IL-6, and tumor necrosis factor-alpha [TNF-α]), when controlling for age and educational level. When the analyses were repeated using sleep quality subscale scores, the poor sleep quality subscale score was positively related to increased pro-inflammatory (IL-2, IL-6, and TNF-α) and anti-inflammatory (IL-10) cytokine levels. Longer sleep latency was positively related to increased pro-inflammatory and sleep regulating cytokines (IL-1β and TNF-α). The sleep disturbance subscale score was positively related to increased pro-inflammatory IL-6, which is known to disrupt sleep at increased concentrations. Shorter sleep duration was positively related to TNF-α. Poor sleep efficiency was not related to any pro or anti-inflammatory cytokine studied. When examining the moderating effect of depression, poor global sleep quality’s relationship to IL-4 and IL-6 was positively moderated by depressive symptom severity such that poorer sleep related to greater IL-4 and IL-6 to a significantly greater degree in women reporting more depressive symptoms. Additionally, sleep duration relationship to IL-4 was also positively moderated by depression symptom status such that the association between shorter sleep duration and IL-4 was significantly greater for more depressed women. Importantly, depression, fatigue severity and interference, and CFS symptom severity and frequency were not related to any cytokine. In conclusion, poorer sleep quality is associated with greater fatigue, CFS symptoms, and inflammation is significant in CFS/ME women, and may be more pronounced in CFS/ME women who are more depressed. These preliminary and novel findings deserve further research using longitudinal study designs to establish the temporality of these associations, and may shed light on the biopsychosocial mechanisms underlying this chronic condition. This work also suggests that subgroups within this heterogeneous population (e.g., depressed women) may respond in distinctive ways to sleep disruptions, which could inform intervention strategies.

Title:
Treatment with chondroitin sulfate to modulate inflammation and atherogenesis in obesity

Publication:
Atherosclerosis. (2016) doi.org/10.1016/j.atherosclerosis.2015.12.016

Author(s):
Pedro Melgar-Lesmesa, Fernando Garcia-Politea, Paula Del-Rey-Puecha, et al.

Quansys Products Used:
Q-Plex Mouse Array

Abstract:

Title:
A vaccine consisting of Schistosoma mansoni cathepsin B formulated in Montanide ISA 720 VG induces high level protection against murine schistosomiasis

Publication:
BMC Infectious Diseases. (2016) doi.org/10.1186/s12879-016-1444-z

Author(s):
Alessandra Ricciardi, Kittipos Visitsunthorn, John P. Dalton, et al.

Quansys Products Used:
Mouse Cytokine Screen (16-plex)

Abstract:

Show Abstract (145 words)

Title:
EGFR transactivation contributes to neuroinflammation in Streptococcus suis meningitis

Publication:
Journal of Neuroinflammation. 2016: doi.org/10.1186/ s12974-016-0734-0

Author(s):
Xiao-Pei Yang, Ji-Yang Fu, Rui-Cheng Yang, et al.

Quansys Products Used:
Q-Plex™ Chemiluminescent ELISA

Abstract:

Title:
Altered Serum Cytokine Profiles in Relapse Phase of Relapsing-Remitting Multiple Sclerosis

Publication:
Iranian Journal of Immunology. 2016.

Author(s):
Forooz Peiravian, Hamid Rajaian, Afshin Samiei, et al.

Quansys Products Used:
Multiplex Human Cytokine Strip Wells (16-plex)

Abstract:

Show Abstract (167 words)

Title:
Analgesic Effect of Indian Gooseberry (Emblica officinalis Fruit) Extracts on Postoperative and Neuropathic Pain in Rats

Publication:
Nutrients. 2016: doi.org/10.3390/nu8120760

Author(s):
Dong Wook Lim, Jae Goo Kim, Yun Tai Kim

Quansys Products Used:
Multiplex ELISA Cytokine Assays

Abstract:

Show Abstract (164 words)

Title:
Immunochemical Assays and Nucleic-Acid Detection Techniques for Clinical Diagnosis of Prostate Cancer

Publication:
J Cancer. 2016: doi.org/10.7150/jca.13821

Author(s):
Prosper Kanyong, Sean Rawlinson, and James Davis.

Quansys Products Used:
Multiplex ELISA

Abstract:

Show Abstract (159 words)

Title:
Iron oxide nanoparticles for use in treating non-infectious inflammatory disorders

Publication:
Ramot At Tel Aviv University Ltd. 2016

Author(s):
Jonathan Leor, Tamar Ben-Mordechai, Shimrit Adutler-Lieber, et al.

Quansys Products Used:
16-plex Multiplex ELISA

Abstract:

Show Abstract (48 words)

Title:
Gut dendritic cell activation links an altered colonic microbiome to mucosal and systemic T-cell activation in untreated HIV-1 infection

Publication:
Mucosal Immunology. 2016: doi.org/ 10.1038/mi.2015.33

Author(s):
S. M. Dillon, E. J. Lee, C. V. Kotter, et al.

Quansys Products Used:
Custom Q-plex Array

Abstract:

Show Abstract (182 words)

Title:
Effects of Proton and Combined Proton and Fe Radiation on the Hippocampus

Publication:
Radiation Research. 2016: doi.org/10.1667/RR14222.1

Author(s):
Jacob Raber, Antiño R. Allen, Sourabh Sharma, et al.

Quansys Products Used:
Q-Plex Mouse Cytokine Array

Abstract:

Show Abstract (379 words)

The space radiation environment contains protons and ⁵⁶Fe, which could pose a significant hazard to space flight crews during and after missions. The space environment involves complex radiation exposures, thus, the effects of a dose of protons might be modulated by a dose of heavy-ion radiation. The brain, and particularly the hippocampus, may be susceptible to space radiation-induced changes. In this study, we first determined the dose-response effect of proton radiation (150 MeV) on hippocampus-dependent cognition 1 and 3 months after exposure. Based on those results, we subsequently exposed mice to protons alone (150 MeV, 0.1 Gy), ⁵⁶Fe alone (600 MeV/n, 0.5 Gy) or combined proton and ⁵⁶Fe radiations (protons first) with the two exposures separated by 24 h. At one month postirradiation, all animal groups showed novel object recognition. However, at three months postirradiation, mice exposed to either protons or combined proton and ⁵⁶Fe radiations showed impaired novel object recognition, which was not observed in mice irradiated with ⁵⁶Fe alone. The mechanisms in these impairments might involve inflammation. In mice irradiated with protons alone or ⁵⁶Fe alone three months earlier, there was a negative correlation between a measure of novel object recognition and the number of newly born activated microglia in the dentate gyrus. Next, cytokine and chemokine levels were assessed in the hippocampus. At one month after exposure the levels of IL-12 were higher in mice exposed to combined radiations compared with sham-irradiated mice, while the levels of IFN-γ were lower in mice exposed to ⁵⁶Fe radiation alone or combined radiations. In addition, IL-4 levels were lower in ⁵⁶Fe-irradiated mice compared with proton-irradiated mice and TNF-α levels were lower in proton-irradiated mice than in mice receiving combined radiations. At three months after exposure, macrophage-derived chemokine (MDC) and eotaxin levels were lower in mice receiving combined radiations. The levels of MDC and eotaxin correlated and the levels of MDC, but not eotaxin, correlated with the percentage of newly born activated microglia in the blades of the dentate gyrus. Finally, hippocampal IL-6 levels were higher in mice receiving combined radiations compared with mice receiving ⁵⁶Fe radiation alone. These data demonstrate the sensitivity of novel object recognition for detecting cognitive injury three months after exposure to proton radiation alone, and combined exposure to proton and ⁵⁶Fe radiations, and that newly-born activated microglia and inflammation might be involved in this injury.

2015 Publications

Title:
The Novel Immunotherapeutic Oligodeoxynucleotide IMT504 Protects Neutropenic Animals from Fatal Pseudomonas aeruginosa Bacteremia and Sepsis

Publication:
Antimicrob. Agents Chemother. February 2015 vol. 59 no. 2 1225-1229

Author(s):
Abdullah Chahin, Steven Opal, Jorge Zorzopulos, et al.

Quansys Products Used:
Rat Cytokine Inflammation Array

Abstract:

Show Abstract (234 words)

Title:
The histone deacetylase inhibitor panobinostat lowers biomarkers of cardiovascular risk and inflammation in HIV patients

Publication:
AIDS. (2015). DOI:10.1097/QAD.0000000000000678

Author(s):
A Kjaer, C Brinkmann, C Dinarello, et al.

Quansys Products Used:
Human Cytokine Array (8-Plex)

Abstract:

Show Abstract (143 words)

Title:
Effect of Acer tegmentosum bark on atopic dermatitis-like skin lesions in NC/Nga mice

Publication:
Journal of Ethnopharmacology, (2015). doi.org/10.1016/j.jep.2015.10.033

Author(s):
Gabsik Yang, Duckgan An, Mi-Hwa Lee, et al.

Quansys Products Used:
Mouse Cytokine Screen (16-Plex) and Q-View Imager

Abstract:

Title:
A combination of isolated phytochemicals and botanical extracts lowers diastolic blood pressure in a randomized controlled trial of hypertensive subjects

Publication:
EJCN (2015). doi:10.1038/ejcn.2015.88

Author(s):
S Biesinger, H A Michaels, A S Quadros, et al.

Quansys Products Used:
Human Cytokine

Abstract:

Show Abstract (111 words)

Title:
TLR4 Expression by Liver Resident Cells Mediates the Development of Glucose Intolerance and Insulin Resistance in Experimental Periodontitis

Publication:
Plos One: 2015. doi.org/10.1371/journal.pone.0136502

Author(s):
Vladimir Ilievski, Yale Cho, Priya Katwala, et al.

Quansys Products Used:
Sample Testing Services

Abstract:

Show Abstract (140 words)

Title:
STAT3 signaling contributes to the high effector activities of interleukin-15-derived dendritic cells

Publication:
Immunol Cell Biol. 2015, doi.org/10.1038/icb.2014.103

Author(s):
Okada S, Han S, Patel ES, et al.

Quansys Products Used:
Sample Testing Service

Abstract:

Show Abstract (276 words)

Title:
The Effects of Telephone-Delivered Cognitive Behavioral Stress Management on Inflammation and Symptoms in Myalgic Enchephalomyelitis/Chronic Fatigue Syndrome: A Computational Immunology Approach

Publication:
Open Access Dissertations, 2015. Paper 1445.

Author(s):
Emily G. Lattie

Quansys Products Used:
Q-Plex Human Cytokine – Screen (16-Plex), Q-View Imager, Q-View Software

Abstract:

Show Abstract (491 words)

Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) is a multi-systemic and unpredictable medical illness for which effective treatments are lacking. A number of studies have documented immune dysregulation in this patient population, and past research has demonstrated the benefits of stress management training on immune functioning. Due to unpredictable and often severe symptoms, patients with ME/CFS have reported difficulties in attending appointments outside of the home. In consideration of these difficulties, this patient population may particularly benefit from therapeutic services available from one’s own home. This study aimed to examine the impact of telephone-delivered CBSM on inflammation and symptoms in individuals with ME/CFS. In order to determine the relationships between cytokine network coexpression patterns and CFS symptoms, the first study examined the presence of cytokine network coexpression patterns and symptom patterns in 215 women diagnosed with ME/CFS. Principal component analyses of 16 cytokines demonstrated a two-component solution in which the first component was characterized primarily by the presence of pro-inflammatory and Th1 cytokines, while the second component was primarily characterized by absence of pro-inflammatory and Th1 cytokines and presence of anti-inflammatory IL-13. Examination of 20 common ME/CFS symptoms revealed three distinct clusters of ME/CFS symptoms: Fatigue/Sleep Impairments, Pain/Neurological Impairments and Immune/Gastrointestinal Impairments. Attempts to model cytokine components and symptom clusters together revealed that while cytokines shared a substantial amount of variability with one another and the variables within each symptom cluster were highly correlated with one another, there were few direct pathways, or “links” between the cytokines and symptoms. Direct relationships were observed between IL-1α and the symptoms chills and sinus or nasal symptoms, IL-6 and the symptom of severe headaches, IL-17 and the symptom problems getting to sleep or problems waking up early in the morning, and IFN-γ and the symptom eyes extremely sensitive to light. In a second study, longitudinal differences in cytokines and symptoms were examined between participants who received telephone-delivered CBSM (N = 53) and participants who received a telephone-delivered health promotion program (N = 40) as part of a randomized controlled trial. No group by time effects were detected in changes in cytokines or symptoms over a 9-month follow-up, which may have been due to both the use of a stronger control condition and a weaker intervention, as group CBSM participants may have had difficulty engaging with the intervention material over the telephone. However, there were significant changes across time in both groups in the cytokine components (the first described the presence of pro-inflammatory and Th1 cytokines, while the second component was primarily characterized by absence of pro-inflammatory and Th1 cytokines and presence of anti-inflammatory IL-13) and in the Fatigue/Sleep Impairments symptom cluster. Mechanisms for change over this period may include increased self-efficacy to solve one’s problems, resulting in reduced stress and enhanced self-care. Given the barriers to treatment faced by many individuals with ME/CFS, future therapeutic interventions for this population should examine the effects of alternate modes of intervention delivery such as home-based video-conferencing or module-based websites.

Title:
In vitro studies of antifibrotic and cytoprotective effects elicited by proto-berberine alkaloids in human dermal fibroblasts

Publication:
Pharmacological Reports, 2015, doi.org/10.1016/j.pharep.2015.04.001

Author(s):
Daniele Pietra, Alice Borghini, Anna Maria Bianucci

Quansys Products Used:
Q-View Software

Abstract:

Show Abstract (158 words)

Title:
Study on the Usefulness of APR Scores from the Viewpoint of Proinflammatory Cytokines

Publication:
Disease Markers (2015), doi.org/10.1155/2015/981981

Author(s):
Nakamura T, Hatanaka D, Yoshioka T, et al.

Quansys Products Used:
Human Cytokine – Inflammation (9-Plex)

Abstract:

Show Abstract (218 words)

Title:
Lymphocytes from chronically stressed mice confer antidepressant-like effects to naive mice

Publication:
J Neurosci. 2015, doi.org/10.1523/JNEUROSCI.2278-14.2015

Author(s):
Brachman RA, Lehmann ML, Maric D, et al.

Quansys Products Used:
Mouse Cytokine – Screen (16-Plex)

Abstract:

Show Abstract (240 words)

Title:
Stress and the Multiple-Role Woman: Taking a Closer Look at the “Superwoman”

Publication:
PLOS One 2015, doi.org/10.1371/journal.pone.0120952

Author(s):
Monika K. Sumra , Michael A. Schillaci

Quansys Products Used:
Human Cytokine Array

Abstract:

Show Abstract (289 words)

Title:
Defining CD4 T Cell Memory by the Epigenetic Landscape of CpG DNA Methylation

Publication:
The Journal of Immunology February 15, 2015, doi.org/10.4049/jimmunol.1401162

Author(s):
H. Kiyomi Komori, Traver Hart, Sarah A. LaMere, et al.

Quansys Products Used:
Human Cytokine Screen IR and Q-View Software

Abstract:

Title:
Toosendan Fructus ameliorates the pancreatic damage through the anti-inflammatory activity in non-obese diabetic mice

Publication:
Kor. J. Herbology, 2015

Author(s):
Seong-Soo Roh, Yong-Ung Kim

Quansys Products Used:
Mouse Cytokine Array

Abstract:

Show Abstract (112 words)

Title:
The Anti-Inflammatory Activity of a Novel Fused-Cyclopentenone Phosphonate and Its Potential in the Local Treatment of Experimental Colitis

Publication:
Gastroenterology Research and Practice, 2015. doi.org/10.1155/2015/939483

Author(s):
Dorit Moradov, Helena Shifrin, Efrat Harel, et al.

Quansys Products Used:
Q-Plex Cytokine Array

Abstract:

Show Abstract (166 words)

Title:
Immune Memory to Sudan Virus: Comparison between Two Separate Disease Outbreaks

Publication:
Viruses 2015, 7(1), 37-51; doi.org/10.3390/v7010037

Author(s):
Ariel Sobarzo, Yael Eskira, Andrew S. Herbert, et al.

Quansys Products Used:
Human Cytokine Array, Q-View Imager, Q-View Software

Abstract:

Show Abstract (274 words)

Recovery from ebolavirus infection in humans is associated with the development of both cell-mediated and humoral immune responses. According to recent studies, individuals that did not survive infection with ebolaviruses appear to have lacked a robust adaptive immune response and the expression of several early innate response markers. However, a comprehensive protective immune profile has yet to be described. Here, we examine cellular memory immune responses among survivors of two separate Ebolavirus outbreaks (EVDs) due to Sudan virus (SUDV) infection in Uganda—Gulu 2000–2001 and Kibaale 2012. Freshly collected blood samples were stimulated with inactivated SUDV, as well as with recombinant SUDV or Ebola virus (EBOV) GP (GP_1–649). In addition, ELISA and plaque reduction neutralization assays were performed to determine anti-SUDV IgG titers and neutralization capacity. Cytokine expression was measured in whole blood cultures in response to SUDV and SUDV GP stimulation in both survivor pools, demonstrating recall responses that indicate immune memory. Cytokine responses between groups were similar but had distinct differences. Neutralizing, SUDV-specific IgG activity against irradiated SUDV and SUDV recombinant proteins were detected in both survivor cohorts. Furthermore, humoral and cell-mediated crossreactivity to EBOV and EBOV recombinant GP_1–649 was observed in both cohorts. In conclusion, immune responses in both groups of survivors demonstrate persistent recognition of relevant antigens, albeit larger cohorts are required in order to reach greater statistical significance. The differing cytokine responses between Gulu and Kibaale outbreak survivors suggests that each outbreak may not yield identical memory responses and promotes the merits of studying the immune responses among outbreaks of the same virus. Finally, our demonstration of cross-reactive immune recognition suggests that there is potential for developing cross-protective vaccines for ebolaviruses.

Title:
Evaluation of the immune response and protective efficacy of Schistosoma mansoni Cathepsin B in mice using CpG dinucleotides as adjuvant

Publication:
Vaccine Volume 33, Issue 2, 3 January 2015, Pages 346–353

Author(s):
Alessandra Ricciardia, John P. Daltonc, Momar Ndaoa

Quansys Products Used:
Mouse Cytokine Array

Abstract:

Show Abstract (220 words)

Title:
Immune Tolerance Induction against Experimental Autoimmune Encephalomyelitis (EAE) Using A New PLP-B7AP Conjugate that Simultaneously Targets B7/CD28 Costimulatory Signal and TCR/MHC-II Signal

Publication:
J Mult Scler (Foster City). 2015 doi.org/10.4172/2376-0389.1000131

Author(s):
Ahmed Badawi, Paul Kiptoo, Teruna Siahaan

Quansys Products Used:
Custom Q-Plex Array

Abstract:

Show Abstract (235 words)

Title:
Cytokines in cerebrospinal fluid of neurosyphilis patients: Identification of Urokinase plasminogen activator using antibody microarrays

Publication:
Journal of Neuroimmunology. 2015. doi.org/10.1016/j.jneuroim.2015.12.010

Author(s):
Ping Lu, Dao-Cheng Zheng, Chang Fang, et al.

Quansys Products Used:
Q-View Imager and Q-View Software

Abstract:

Title:
Neurological Response to cART vs. cART plus Integrase Inhibitor and CCR5 Antagonist Initiated during Acute HIV

Publication:
Plos One: 2015, doi.org/10.1371/journal.pone.0142600

Author(s):
Victor Valcour, Serena Spudich, Napapon Sailasuta, et al.

Quansys Products Used:
Custom Q-Plex Array and Q-View Software

Abstract:

Show Abstract (92 words)

Title:
Serum cytokines as biomarkers for age-related macular degeneration

Publication:
Graefe’s Archive for Clinical and Experimental Ophthalmology. May 2015, Volume 253, Issue 5, pp 699-704

Author(s):
Khaled Nassar, Salvatore Grisanti, Elshaymaa Elfar, et al.

Quansys Products Used:
Human Cytokine (16-Plex), Q-View Imager, Q-View Software

Abstract:

Show Abstract (169 words)

Title:
The Immunology of a Healing Response in Cutaneous Leishmaniasis Treated with Localized Heat or Systemic Antimonial Therapy

Publication:
Plos One: 2015, doi.org/10.1371/journal.pntd.0004178

Author(s):
Ines Lakhal-Naouar, Bonnie M. Slike, Naomi E. Aronson , et al.

Quansys Products Used:
Human Cytokine–IR Array

Abstract:

Show Abstract (89 words)

Title:
Pancreastatin-Dependent Inflammatory Signaling Mediates Obesity-Induced Insulin Resistance

Publication:
Diabetes, 2015, doi.org/10.2337/db13-1747

Author(s):
Gautam K. Bandyopadhyay, Minh Lu, Ennio Avolio, et al.

Quansys Products Used:
Q-Plex Array

Abstract:

Title:
Caffeine promotes anti-tumor immune response during tumor initiation: Involvement of the adenosine A2A receptor

Publication:
Biochemical Pharmacology, 2015, doi.org/10.1016/j.bcp.2015.08.092

Author(s):
Hadar Einia, Valeria Frishmana, Robert Yulzarib, et al.

Quansys Products Used:
Custom Q-Plex Array

Abstract:

Show Abstract (243 words)

Title:
Effect of Negative Pressure Therapy on the Inflammatory Response of the Intestinal Microenvironment in a Porcine Septic Model

Publication:
Mediators of Inflammation, 2015, doi.org/10.1155/2015/419841

Author(s):
Kenneth C. Norbury and Mary Pat Moyer

Quansys Products Used:
Q-Plex Array, Q-View Imager, Q-View Software

Abstract:

Show Abstract (190 words)

Title:
CCR2 Antagonism Alters Brain Macrophage Polarization and Ameliorates Cognitive Dysfunction Induced by Traumatic Brain Injury

Publication:
J Neurosci. 2015. doi.org/10.1523/JNEUROSCI.2405-14.2015

Author(s):
Morganti JM, Jopson TD, Liu S, et al.

Quansys Products Used:
Mouse Cytokine Array, Q-View Software

Abstract:

Title:
Female PAPP-A knockout mice are resistant to metabolic dysfunction induced by high-fat/high-sucrose feeding at middle age

Publication:
AGE. 2015. doi.org/10.1007/s11357-015-9765-1

Author(s):
Cristal Hill, Oge Arum, Ravneet Boparai, et al.

Quansys Products Used:
Mouse Cytokine (4-Plex) and Q-View Software

Abstract:

Show Abstract (350 words)

Longevity and aging are influenced by common intracellular signals of the insulin/insulin-like growth factor (IGF)-1 pathway. Abnormally high levels of bioactive IGF-1 increase the development of various cancers and may contribute to metabolic diseases such as insulin resistance. Enhanced availability of IGF-1 is promoted by cleavage of IGF binding proteins (IGFBPs) by proteases, including the pregnancy-associated plasma protein-A (PAPPA). In vitro, PAPP-A is regulated by pro-inflammatory cytokines (PICs) such as interleukin (IL)-6 and tumor necrosis factor (TNF). Mice born with deficiency of the Papp-a gene (PAPP-A knockout (KO) mice) live ∼30–40 % longer than their normal littermates and have decreased bioactive IGF-1 on standard diets. Our objective was to elucidate how the effects of high-fat, high-sucrose diet (HFHS) promote obesity, induce metabolic dysfunction, and alter systemic cytokine expression in PAPP-A KO and normal mice. PAPP-A KO mice fed HFHS diet for 10 weeks were more glucose tolerant and had enhanced insulin sensitivity compared to normal mice fed HFHS diet. PAPP-A KO mice fed HFHS diet had lower levels of pro-inflammatory cytokines (IL-2, IL-6, and TNF-α) compared to normal mice fed the same diet. However, anti-inflammatory cytokine levels (IL-4 and adiponectin) were higher in PAPP-A KO mice fed HFHS diet compared to normal mice fed HFHS. Circulating PAPP-A levels were elevated in normal mice fed an HFHS diet compared to normal mice fed a standard, low-fat, low-sucrose (LFLS) diet. Indirect calorimetry showed, at 10 weeks of feeding HFHS diet, significantly increased oxygen consumption (VO₂) in PAPP-A KO mice fed HFHS diet compared to normal mice fed the same diet. Furthermore, respiratory quotient (RQ) was significantly lower in PAPP-A KO mice fed HFHS diet compared to normal (N) mice fed HFHS diet indicating PAPP-A KO mice fed HFHS diet are able to rely on fat as their primary source of energy more so than normal controls. We conclude that PAPP-A KO mice are resistant to the HFHS diet induction of metabolic dysfunction associated with higher levels of anti-inflammatory cytokines and a remarkably metabolic flexible phenotype and that some of the effects of HFHS diet in normal animals may be due to increased levels of PAPP-A.

Title:
Impaired RASGRF1/ERK–mediated GM-CSF response characterizes CARD9 deficiency in French-Canadians

Publication:
Journal of Allergy and Clinical Immunology

Author(s):
Christina Gavino, Nancy Hamel, Ji Bin Zeng, et al.

Quansys Products Used:
Cytokine Q-Plex Array

Abstract:

Show Abstract (93 words)

Title:
Therapeutic Compositions and Methods for Treating Infections

Publication:
US Patent Application 20150352160

Author(s):
Rick Tarleton, Samarchith Kurup

Quansys Products Used:
Mouse Cytokine Screen ELISA and Q-View Software

Abstract:

Show Abstract (86 words)

Title:
Analgesic Effect of Ilex paraguariensis Extract on Postoperative and Neuropathic Pain in Rats

Publication:
Biological and Pharmaceutical Bulletin. (2015). doi.org/10.1248/bpb.b15-00360

Author(s):
Dong Wook Lim, Jae Goo Kim, Taewon Han, et al.

Quansys Products Used:
Q-Plex Cytokine Array

Abstract:

Show Abstract (196 words)

Title:
In vitro biophysical, microspectroscopic and cytotoxic evaluation of metastatic and non-metastatic cancer cells in responses to anti-cancer drug

Publication:
Analytical Methods. (2015) DOI: 10.1039/C5AY01810B

Author(s):
Qifei Li, Lifu Xiao, Sitaram Harihar, et al.

Quansys Products Used:
Q-Plex Cytokine and Chemokine Kits

Abstract:

Show Abstract (180 words)

Title:
Immune-Modifying Nanoparticles for the Treatment of Inflammatory Diseases

Publication:
FPO, January 2015

Author(s):
Daniel Getts

Quansys Products Used:
Mouse Cytokine Screen IR (16-plex) and Q-View Software

Abstract:

Show Abstract (41 words)

Title:
Interleukins 6 and 15 Levels Are Higher in Subcutaneous Adipose Tissue, but Obesity Is Associated with Their Increased Content in Visceral Fat Depots

Publication:
Int. J. Mol. Sci. 2015, doi.org/10.3390/ijms161025817

Author(s):
Marta Jonas, Alina Kurylowicz, Zbigniew Bartoszewicz, et al.

Quansys Products Used:
Custom Q-Plex Array and Q-View Software

Abstract:

Show Abstract (207 words)

Title:
Exercise-mimetic AICAR transiently benefits brain function

Publication:
Oncotarget. 2015. doi.org/10.18632/oncotarget.4715

Author(s):
Davide Guerrieri, Henriette van Praag

Quansys Products Used:
Mouse Cytokine IR (9-Plex) and Q-View Software

Abstract:

Show Abstract (247 words)

Title:
Collection of Wound Exudate From Human Digit Tip Amputations Does Not Impair Regenerative Healing

Publication:
Medicine (Baltimore). 2015 DOI: 10.1097/MD.0000000000001764

Author(s):
Tobias Kisch, Julia Maria Klemens, Katharina Hofmann, et al.

Quansys Products Used:
Q-Plex Array and Q-View Software

Abstract:

Show Abstract (254 words)

Title:
Treatment with chondroitin sulfate to modulate inflammation and atherogenesis in obesity

Publication:
Atherosclerosis. 2015, doi.org/10.1016/j.atherosclerosis.2015.12.016

Author(s):
Pedro Melgar-Lesmes, Fernando Garcia-Polite, Paula Del-Rey-Puech, et al.

Quansys Products Used:
Sample Testing Service

Abstract:

Show Abstract (221 words)

Title:
IGF1R- and ROR1-Specific CAR T Cells as a Potential Therapy for High Risk Sarcomas

Publication:
Plos One: 2015, doi.org/10.1371/journal.pone.0133152

Author(s):
Xin Huang, Haein Park, Joseph Greene, et al.

Quansys Products Used:
Human Cytokines Screen (16-Plex)

Abstract:

Title:
Nitric Oxide Synthase Inhibition Modulates Immune Polarization and Improves Radiation-Induced Tumor Growth Delay

Publication:
The Journal of Cancer Research, 2015. doi.org/10.1158/0008-5472.CAN-14-3011

Author(s):
Lisa A Ridnour, Robert YS Cheng, Jonathan M Weiss, et al.

Quansys Products Used:
Q-Plex Cytokine Array

Abstract:

Show Abstract (248 words)

Title:
Loss of CCR2 expressing non-classical monocytes are associated with cognitive impairment in antiretroviral therapy-naïve HIV-infected Thais

Publication:
Journal of Neuroimmunology, 2015, doi.org/10.1016/j.jneuroim.2015.08.020

Author(s):
Lishomwa C. Ndhlovua, Michelle L. D’Antonia, et al.

Quansys Products Used:
Custom Q-Plex Array and Q-View Software

Abstract:

Show Abstract (103 words)

Title:
Is Eotaxin-1 a serum and urinary biomarker for prostate cancer detection and recurrence?

Publication:
Prostate ,2015, doi.org/10.1002/pros.23086

Author(s):
Heidegger, I., Höfer, J., Luger, M., et al.

Quansys Products Used:
Custom Q-Plex Array and Q-View Software

Abstract:

Show Abstract (150 words)

Title:
AS03-adjuvanted, very-low-dose influenza vaccines induce distinctive immune responses compared to unadjuvanted high-dose vaccines in BALB/c mice

Publication:
Front Immunol. 2015, doi.org/10.3389/fimmu.2015.00207.eCollection

Author(s):
Yam KK, Gupta J, Winter K, et al.

Quansys Products Used:
Mouse Cytokine – Screen (16-Plex)

Abstract:

Show Abstract (189 words)

Title:
Molecular effects of lithium are partially mimicked by inositol-monophosphatase (IMPA)1 knockout mice in a brain region-dependent manner

Publication:
European Neuropsychopharmacology. 2015. doi.org/10.1016/j.euroneuro.2014.06.012

Author(s):
O. Damri, Y. Sade, L. Toker, et al.

Quansys Products Used:
Mouse Cytokine Array

Abstract:

Show Abstract (211 words)

Title:
Cannabinoid receptor-specific mechanisms to ameliorate pain in sickle cell anemia via inhibition of mast cell activation and neurogenic inflammation

Publication:
Haematologica 2015. doi:10.3324/haematol.2015.136523

Author(s):
Lucile Vincent, Derek Vang, Julia Nguyen, et al.

Quansys Products Used:
Sample Testing Services

Abstract:

Show Abstract (156 words)

Title:
Glucocorticoid Enhances Viability of Human Respiratory Epithelial Cells Inflicted by Ambient Particulate Matter

Publication:
Bulletin of the Korean Chemical Society. 2015. doi.org/10.1002/bkcs.10251

Author(s):
Nam Heo, Ahruem Baek, Yu Mi Baek, et al.

Quansys Products Used:
Human Cytokine

Abstract:

Show Abstract (229 words)

Title:
The Morphological and Molecular Changes of Brain Cells Exposed to Direct Current Electric Field Stimulation

Publication:
International Journal of Neuropsychopharmacology (2015). doi.org/10.1093/ijnp/pyu090

Author(s):
Simon Pelletier, Marie Lagace, Isabelle St-Amour, et al.

Quansys Products Used:
Mouse Cytokine Screen (16-Plex) and Q-View Software

Abstract:

Show Abstract (127 words)

Title:
The Activation-Induced Assembly of an RNA/Protein Interactome Centered on the Splicing Factor U2AF2 Regulates Gene Expression in Human CD4 T Cells

Publication:
Plos One: 2015. DOI: 10.1371/journal.pone.0144409

Author(s):
Thomas Whisenant, Eigen Peralta, Lauren Aarreberg, et al.

Quansys Products Used:
Human Cytokine Screen IR (16-Plex) and Q-View Software

Abstract:

Show Abstract (288 words)

Activation of CD4 T cells is a reaction to challenges such as microbial pathogens, cancer and toxins that defines adaptive immune responses. The roles of T cell receptor crosslinking, intracellular signaling, and transcription factor activation are well described, but the importance of post-transcriptional regulation by RNA-binding proteins (RBPs) has not been considered in depth. We describe a new model expanding and activating primary human CD4 T cells and applied this to characterizing activation-induced assembly of splicing factors centered on U2AF2. We immunoprecipitated U2AF2 to identify what mRNA transcripts were bound as a function of activation by TCR crosslinking and costimulation. In parallel, mass spectrometry revealed the proteins incorporated into the U2AF2-centered RNA/protein interactome. Molecules that retained interaction with the U2AF2 complex after RNAse treatment were designated as “central” interactome members (CIMs). Mass spectrometry also identified a second class of activation-induced proteins, “peripheral” interactome members (PIMs), that bound to the same transcripts but were not in physical association with U2AF2 or its partners. siRNA knockdown of two CIMs and two PIMs caused changes in activation marker expression, cytokine secretion, and gene expression that were unique to each protein and mapped to pathways associated with key aspects of T cell activation. While knocking down the PIM, SYNCRIP, impacts a limited but immunologically important set of U2AF2-bound transcripts, knockdown of U2AF1 significantly impairs assembly of the majority of protein and mRNA components in the activation-induced interactome. These results demonstrated that CIMs and PIMs, either directly or indirectly through RNA, assembled into activation-induced U2AF2 complexes and play roles in post-transcriptional regulation of genes related to cytokine secretion. These data suggest an additional layer of regulation mediated by the activation-induced assembly of RNA splicing interactomes that is important for understanding T cell activation.

Title:
Invasive Salmonella Typhimurium ST313 with Naturally Attenuated Flagellin Elicits Reduced Inflammation and Replicates within Macrophages

Publication:
PLOS 2015, doi.org/10.1371/journal.pntd.0003394

Author(s):
Girish Ramachandran, Darren J. Perkins, Patrick J. Schmidlein, et al.

Quansys Products Used:
Q-Plex Cytokine Array

Abstract:

Show Abstract (252 words)

2014 Publications

Title:
Hydroxychloroquine Inhibits Autophagy to Potentiate Antiestrogen Responsiveness in ER+ Breast Cancer

Publication:
Clin Cancer Res, 2014. doi.org/10.1158/1078-0432.CCR-13-3227

Author(s):
Katherine L. Cook, Anni Wärri, David R. Soto-Pantoja, et al.

Quansys Products Used:
Mouse Cytokine and Chemokine Array

Abstract:

Show Abstract (216 words)

Title:
Evaluation of the immune response and protective efficacy of Schistosoma mansoni Cathepsin B in mice using CpG dinucleotides as adjuvant

Publication:
Vaccine, Volume, 2014. doi.org/10.1016/j.vaccine.2014.11.016

Author(s):
Alessandra Ricciardia, John P. Daltonc, Momar Ndaoa

Quansys Products Used:
Mouse Cytokine Array

Abstract:

Show Abstract (220 words)

Title:
Early post-stressor intervention with minocycline, a second-generation tetracycline, attenuates post-traumatic stress response in an animal model of PTSD

Publication:
European Neuropsychopharmacology, 2014. doi.org/10.1016/j.euroneuro.2014.11.012

Author(s):
Yechiel Levkovitza, Daphna Fenchela, Zeev Kaplanb, et al.

Quansys Products Used:
Multiplex Array

Abstract:

Title:
Sphingolipid Pathway Regulates Innate Immune Responses at the Fetomaternal Interface during Pregnancy

Publication:
J. Biol. Chem, 2014. doi.org/10.1074/jbc.M114.628867

Author(s):
Kiyomi Mizugishi, Takuya Inoue, Hiroshi Hatayama, et al.

Quansys Products Used:
Mouse Chemokine and Cytokine Arrays

Abstract:

Show Abstract (219 words)

Title:
Pathways Mediating the Interaction between Endothelial Progenitor Cells (EPCs) and Platelets

Publication:
Plos One, 2014. doi.org/10.1371/journal.pone.0095156

Author(s):
Oshrat Raz, Dorit L. Lev, Alexander Battler, Eli I. Lev

Quansys Products Used:
Q-Plex Array

Abstract:

Show Abstract (144 words)

Title:
Regulation of IL-6 and IL-8 production by reciprocal cell-to-cell interactions between tumor cells and stromal fibroblasts through IL-1α in ameloblastoma

Publication:
Biochemical and Biophysical Research Communications, 2014. doi.org/10.1016/j.bbrc.2014.07.137

Author(s):
Takao Fuchigamia, Toshiro Kibeb, Hirofumi Koyamaa, et al.

Quansys Products Used:
Human Cytokine Array and Q-View Software

Abstract:

Show Abstract (228 words)

Title:
Hydroxychloroquine inhibits autophagy to potentiate antiestrogen responsiveness in ER+ breast cancer

Publication:
Clin Cancer Res, 2014. doi.org/10.1158/1078-0432.CCR-13-3227

Author(s):
Cook KL, Wärri A, Soto-Pantoja DR, et al.

Quansys Products Used:
Sample Testing Service

Abstract:

Show Abstract (216 words)

Title:
Immunologic characterization of a novel inactivated nasal mumps virus vaccine adjuvanted with Protollin

Publication:
Vaccine Volume 32, 2014. doi.org/10.1016/j.vaccine.2013.11.014

Author(s):
Katie R. Younga, Sazini Nzulaa, David S. Burtb, et al.

Quansys Products Used:
Mouse Cytokine Screen 16-plex

Abstract:

Show Abstract (208 words)

Title:
The different effects of LPS and poly I:C prenatal immune challenges on the behavior, development and inflammatory responses in pregnant mice and their offspring

Publication:
Brain, Behavior, and Immunity, 2014. doi.org/10.1016/j.bbi.2013.12.016

Author(s):
Dany Arsenaulta, Isabelle St-Amoura, Giulia Cisbania, et al.

Quansys Products Used:
Q-Plex, Q-View Software

Abstract:

Show Abstract (271 words)

Title:
Simultaneous multi-analyte urinary protein assay for bladder cancer detection

Publication:
BMC Biotechnology, 2014. doi.org/10.1186/1472-6750-14-24

Author(s):
Charles J Rosser, Yunfeng Dai, Makito Miyake, et al.

Quansys Products Used:
Q-Plex, Sample Testing Service, Q-View Software, Q-View Imager

Abstract:

Show Abstract (113 words)

Title:
A Multiplex Immunoassay Method for Simultaneous Quantification of Iron, Vitamin A and Inflammation Status Markers

Publication:
PLOS, 2014. doi.org/10.1371/journal.pone.0115164

Author(s):
Eleanor Brindle, Daniel Stevens, Christopher Crudder, et al.

Quansys Products Used:
Custom Development, Q-View Imager, Q-View Software, Q-Plex Array

Abstract:

Show Abstract (235 words)

Title:
miR-146a and miR-146b Regulate Human Dendritic Cell Apoptosis and Cytokine Production by Targeting of TRAF6 and IRAK1

Publication:
J. Biol. Chem, 2014. doi.org/10.1074/jbc.M114.591420

Author(s):
Haein Park, Xin Huang, Changming Lu, et al.

Quansys Products Used:
Human Cytokine Array

Abstract:

Show Abstract (199 words)

Title:
Molecular and Cellular Characterization of a Salmonella enterica Serovar Paratyphi A Outbreak Strain and the Human Immune Response to Infection

Publication:
Clin Vaccine Immunol, 2014. doi.org/10.1128/CVI.05468-11

Author(s):
Ohad Gal-Mora, Jotham Sueza, Dana Elhadad, et al.

Quansys Products Used:
Q-Plex Chemiluminescent IL-8 kit, Q-View Imager, Q-View Software

Abstract:

Title:
The effect of leukocyte-reduced platelet-rich plasma on the proliferation of autologous adipose-tissue derived mesenchymal stem cells

Publication:
Clinical Hemorheology and Microcirculation, 2014

Author(s):
Markus Loibl, Siegmund Lang, Gero Brockhoff, et al.

Quansys Products Used:
Custom Q-Plex Kit

Abstract:

Title:
IRF7-Dependent Type I Interferon Production Induces Lethal Immune-Mediated Disease in STAT1 Knockout Mice Infected with Lymphocytic Choriomeningitis Virus

Publication:
J. Virol., 2014. doi.org/10.1128/JVI.03117-13

Author(s):
Wen Li, Markus J. Hofer, So Ri Jung, et al.

Quansys Products Used:
Mouse Cytokine Array and Q-View Software

Abstract:

Show Abstract (254 words)

Title:
Positive Relationship between Total Antioxidant Status and Chemokines Observed in Adults

Publication:
Oxidative Medicine and Cellular Longevity, 2014. doi.org/10.1155/2014/693680

Author(s):
Yanli Li, Richard W. Browne, Matthew R. Bonner, et al.

Quansys Products Used:
Human Chemokine ELISA

Abstract:

Title:
Early Postnatal Exposure to Ultrafine Particulate Matter Air Pollution: Persistent Ventriculomegaly, Neurochemical Disruption, and Glial Activation Preferentially in Male Mice

Publication:
Environ Health Perspect, 2014. doi.org/10.1289/ehp.1307984

Author(s):
Joshua L. Allen, Xiufang Liu, Sean Pelkowski, Brian Palmer, et al.

Quansys Products Used:
Mouse Cytokine Array, Q-View Software, Q-View Imager

Abstract:

Show Abstract (128 words)

Title:
Protection against Chikungunya virus induced arthralgia following prophylactic treatment with adenovirus vectored interferon (mDEF201)

Publication:
Antiviral Research, 2014. doi.org/10.1016/j.antiviral.2014.05.004

Author(s):
Ashley Dagleyb, Jane Ennisd, Jeffrey D. Turnerd, et al.

Quansys Products Used:
Mouse Cytokine Array (16 plex), Q-View Imager, Q-View Software

Abstract:

Show Abstract (131 words)

Title:
Cationic lipid nanocarriers activate Toll-like receptor 2 and NLRP3 inflammasome pathways

Publication:
Nanomedicine: Nanotechnology, Biology and Medicine, 2014. doi.org/10.1016/j.nano.2013.12.003

Author(s):
Caroline Lonez, Michel Bessodes, Daniel Scherman et al.

Quansys Products Used:
Mouse Q-plex Array, Q-View Software

Abstract:

Title:
Short Report: Association of IL-15 with Peripheral and Hepatic Insulin Sensitivity in Healthy Middle-Aged Men

Publication:
Open Journal of Endocrine and Metabolic Diseases, 2014. doi.org/10.4236/ojemd.2014.43007

Author(s):
Yuichiro Nishida, Kumpei Tokuyama, et al.

Quansys Products Used:
Human Cytokine Q-Plex

Abstract:

Show Abstract (210 words)

Title:
Objectively measured physical activity and inflammatory cytokine levels in middle-aged Japanese people

Publication:
Preventive Medicine Volume 64, 2014. doi.org/10.1016/j.ypmed.2014.04.004

Author(s):
Yuichiro Nishidaa, Yasuki Higakib, Naoto Taguchia, et al.

Quansys Products Used:
Human Cytokine Array

Abstract:

Show Abstract (130 words)

Title:
Consumption of cranberry beverage improved endogenous antioxidant status and protected against bacteria adhesion in healthy humans: a randomized controlled trial

Publication:
Nutrition Research, 2014. doi.org/10.1016/j.nutres.2014.03.006

Author(s):
Bridget D. Mathison, Lindsey L. Kimble, Kerrie L. Kaspar, et al.

Quansys Products Used:
Human Cytokine Array, Q-View Imager

Abstract:

Show Abstract (220 words)

Title:
Strategic use of serology for the diagnosis of bovine tuberculosis after intradermal skin testing

Publication:
Veterinary Microbiology, 2014. doi.org/10.1016/j.vetmic.2014.02.036

Author(s):
Carmen Casala, Alberto Díez-Guerrierb, Julio Álvareza, et al.

Quansys Products Used:
Q-View Software

Abstract:

Show Abstract (233 words)

Title:
Ferrocifen derivatives that induce senescence in cancer cells: selected examples

Publication:
Journal of Inorganic Biochemistry, 2014. doi.org/10.1016/j.jinorgbio.2014.08.015

Author(s):
Céline Bruyèrea, Véronique Mathieua, and Anne Vessièresc, et al.

Quansys Products Used:
Q-View Software

Abstract:

Show Abstract (179 words)

Title:
Nanoparticle-Mediated Combinatorial Targeting of Multiple Human Dendritic Cell (DC) Subsets Leads to Enhanced T Cell Activation via IL-15–Dependent DC Crosstalk

Publication:
The Journal of Immunology, 2014. doi.org/10.4049/jimmunol.1400489

Author(s):
Kartik Sehgal, Ragy Ragheb, Tarek M. Fahmy, et al.

Quansys Products Used:
Q-View Software

Abstract:

Show Abstract (169 words)

Title:
Serum cytokines as biomarkers for age-related macular degeneration

Publication:
Graefe’s Archive for Clinical and Experimental Ophthalmology, 2014. doi.org/10.1007/s00417-014-2738-8

Author(s):
Khaled Nassar, Salvatore Grisanti, Elshaymaa Elfar, et al.

Quansys Products Used:
Human Cytokine Array

Abstract:

Title:
Macrophage-Derived Chemokine (MDC/CCL22) is a Novel Mediator of Lung Inflammation Following Hemorrhage and Resuscitation

Publication:
Shock, 2014. doi.org/10.1097/SHK.0000000000000253

Author(s):
Jillian R. Richter, Jeffrey M. Sutton, Ritha M. Belizaire, et al.

Quansys Products Used:
Mouse Cytokine Array

Abstract:

Show Abstract (239 words)

Title:
Acute over-the-counter pharmacological intervention does not adversely affect behavioral outcome following diffuse traumatic brain injury in the mouse

Publication:
Experimental Brain Research, 2014. doi.org/10.1007/s00221-014-3948-3

Author(s):
Jordan L. Harrison, Rachel K. Rowe, Bruce F. O’Hara, et al.

Quansys Products Used:
Mouse Cytokine IR Q-Plex Assay

Abstract:

Title:
Novel inflammatory biomarkers of portal pressure in compensated cirrhosis patients

Publication:
Hepatology Volume 59, Issue 3, pages 1052–1059, March 2014

Author(s):
Martina Buck, Guadalupe Garcia-Tsao, Roberto J. Groszmann, et al.

Quansys Products Used:
Human Cytokine – Screen IR (16-Plex)

Abstract:

Title:
IVIg protects the 3xTg-AD mouse model of Alzheimer’s disease from memory deficit and Aβ pathology

Publication:
Journal of Neuroinflammation, 2014. doi.org/10.1186/1742-2094-11-54

Author(s):
Isabelle St-Amour, Isabelle Paré, et al.

Quansys Products Used:
Mouse Cytokine – Screen (16-plex)

Abstract:

Show Abstract (156 words)

2013 Publications

Title:
Surgery-induced inflammation reduces morphine distribution into cerebrospinal fluid

Publication:
FASEB J., 2013.

Author(s):
Yan Wang

Quansys Products Used:
Human Cytokine Inflammation (9-plex)

Abstract:

Show Abstract (117 words)

Title:
Prostacyclin promotes oligodendrocyte precursor recruitment and remyelination after spinal cord demyelination

Publication:
Cell Death and Disease, 2013. doi.org/10.1038/cddis.2013.335

Author(s):
C. Takahashi, R. Muramatsu, H. Fujimura, et al.

Quansys Products Used:
Mouse Cytokine Array

Abstract:

Show Abstract (158 words)

Title:
Human α1-Antitrypsin Binds to Heat-Shock Protein gp96 and Protects from Endogenous gp96-Mediated Injury In vivo

Publication:
Frontiers in Immunology, 2013. doi.org/10.3389/fimmu.2013.00320

Author(s):
David E. Ochayon, Mark Mizrahi, Galit Shahaf, et al.

Quansys Products Used:
Mouse Cytokine and Q-View Software

Abstract:

Show Abstract (274 words)

The extracellular form of the abundant heat-shock protein, gp96, is involved in human autoimmune pathologies. In patients with type 1 diabetes, circulating gp96 is found to be elevated, and is bound to the acute-phase protein, α1-antitrypsin (AAT). The two molecules also engage intracellularly during the physiological folding of AAT. AAT therapy promotes pancreatic islet survival in both transplantation and autoimmune diabetes models, and several clinical trials are currently examining AAT therapy for individuals with type 1 diabetes. However, its mechanism of action is yet unknown. Here, we examine whether the protective activity of AAT is related to binding of extracellular gp96. Primary mouse islets, macrophages, and dendritic cells were added recombinant gp96 in the presence of clinical-grade human AAT (hAAT, Glassia™, Kamada Ltd., Israel). Islet function was evaluated by insulin release. The effect of hAAT on IL-1β/IFNγ-induced gp96 cell-surface levels was also evaluated. In vivo, skin transplantation was performed for examination of robust immune responses, and systemic inflammation was induced by cecal puncture. Endogenous gp96 was inhibited by gp96-inhibitory peptide (gp96i, Compugen Ltd., Israel) in an allogeneic islet transplantation model. Our findings indicate that hAAT binds to gp96 and diminishes gp96-induced inflammatory responses; e.g., hAAT-treated gp96-stimulated islets released less pro-inflammatory cytokines (IL-1β by 6.16-fold and TNFα by 2.69-fold) and regained gp96-disrupted insulin release. hAAT reduced cell activation during both skin transplantation and systemic inflammation, as well as lowered inducible surface levels of gp96 on immune cells. Finally, inhibition of gp96 significantly improved immediate islet graft function. These results suggest that hAAT is a regulator of gp96-mediated inflammatory responses, an increasingly appreciated endogenous damage response with relevance to human pathologies that are exacerbated by tissue injury.

Title:
A trifluoromethyl analogue of celecoxib exerts beneficial effects in neuroinflammation

Publication:
PLoS One, 2013. doi.org/10.1371/journal.pone.0083119

Author(s):
Di Penta A, Chiba A, Alloza I, et al.

Quansys Products Used:
Mouse Cytokine Stripwells (16-plex) and Q-View Software

Abstract:

Show Abstract (172 words)

Title:
PARK2 Mediates Interleukin 6 and Monocyte Chemoattractant Protein 1 Production by Human Macrophages

Publication:
Plos One 7, 2013. doi.org/10.1371/journal.pntd.0002015

Author(s):
L. de Léséleuc, M. Orlova, A. Cobat, et al.

Quansys Products Used:
Human Cytokine Q-Plex, Quansys Q-View Imager and Software

Abstract:

Show Abstract (249 words)

Title:
Use of a multiplex enzyme-linked immunosorbent assay to detect a subpopulation of Mycobacterium bovis–infected animals deemed negative or inconclusive by the single intradermal comparative tuberculin skin test

Publication:
Journal of Veterinary Diagnostic Investigation, 2013. doi.org/10.1177/1040638711403410

Author(s):
Clare Whelan, Eduard Shuralev, Hang Fai Kwok, et al.

Quansys Products Used:
ELISA Plate System and Q-View Imager

Abstract:

Show Abstract (197 words)

Title:
Low levels of circulating alpha-1 antitrypsin are associated with spontaneous abortions

Publication:
Journal of Maternal-Fetal and Neonatal Medicine, 2013. doi.org/10.3109/14767058.2013.801955

Author(s):
Tamar Madar, Galit Shahaf, Eyal Sheiner, et al.

Quansys Products Used:
Q-View Software and Q-View Imager

Abstract:

Show Abstract (92 words)

Title:
Host-related carcinoembryonic antigen cell adhesion molecule 1 promotes metastasis of colorectal cancer

Publication:
Oncogene, 2013. doi.org/10.1038/onc.2012.112

Author(s):
A Arabzadeh, C Chan, A-L Nouvion, et al.

Quansys Products Used:
Cytokine/Chemokine Multiplex ELISA Arrays

Abstract:

Show Abstract (193 words)

Title:
Cocktail Blood Biomarkers: Prediction of Clinical Outcomes in Patients with Acute Ischemic Stroke

Publication:
Eur Neurol, 2013. doi.org/10.1159/000342896

Author(s):
Zeng L., Liu J., Wang L. et al.

Quansys Products Used:
Q-View™ Software

Abstract:

Show Abstract (191 words)

Title:
Immunologic characterization of a novel inactivated nasal mumps virus vaccine adjuvanted with Protollin

Publication:
Vaccine, 2014. doi.org/10.1016/j.vaccine.2013.11.014

Author(s):
Young K, Nzula S, Burt D, and Ward B.

Quansys Products Used:
Mouse Cytokine Screen (16 Plex)

Abstract:

Show Abstract (219 words)

Title:
Molecular changes in bone marrow, tumor and serum after conductive ablation of murine 4T1 breast carcinoma

Publication:
International Journal of Oncology. doi.org/10.3892/ijo.2013.2185

Author(s):
Beata P., Gal S., Sagar V., et al.

Quansys Products Used:
Human Cytokine 16-plex, Q-View Imager, Q-View Software

Abstract:

Show Abstract (262 words)

Title:
Methods and materials for determining the efficacy of prostate cancer therapies

Publication:
Caldera Health Limited, 2013.

Author(s):
Douglas J. Watson, Sydney R. Forster, Jay D. White

Quansys Products Used:
Multiplex ELISA

Abstract:

Show Abstract (67 words)

Title:
Perpetual expression of PAMPs necessary for optimal immune control and clearance of a persistent pathogen

Publication:
Nature Communications, 2013. doi.org/10.1038/ncomms3616

Author(s):
Samarchith P. Kurup & Rick L. Tarleton

Quansys Products Used:
Mouse Cytokine Screen ELISA and Q-View Software

Abstract:

Show Abstract (121 words)

Title:
1′-Acetoxychavicol acetate promotes caspase 3-activated glioblastoma cell death by overcoming enhanced cytokine expression

Publication:
Oncology Lett, 2013. doi.org/10.3892/ol.2013.1292

Author(s):
Musa Williams, Illya Tietzel, Quincy A. Quick

Quansys Products Used:
Human 4-Plex Cytokine Array and Q-View Imager

Abstract:

Show Abstract (143 words)

Title:
Astaxanthin Decreases Inflammatory Biomarkers Associated with Cardiovascular Disease in Human Umbilical Vein Endothelial Cells

Publication:
American Journal of Advanced Food Science and Technology, 2013. doi.org/10.7726/ajafst.2013.1001

Author(s):
Weslee Chew, Bridget D. Mathison, Lindsey L. Kimble, et al.

Quansys Products Used:
Human Cytokine Inflammation 9-Plex and Q-View Software

Abstract:

Title:
Dose-Dependent Effects of IL-17 on IL-13–Induced Airway Inflammatory Responses and Airway Hyperresponsiveness

Publication:
The Journal of Immunology, 2013. doi.org/10.4049/jimmunol.1200506

Author(s):
Margaret W. Kinyanjui, Jichuan Shan, Emily M. Nakada, et al.

Quansys Products Used:
Mouse Q-Plex Chemokine Kit

Abstract:

Show Abstract (241 words)

Title:
Noninvasive pulsed focused ultrasound allows spatiotemporal control of targeted homing for multiple stem cell types in murine skeletal muscle and the magnitude of cell homing can be increased through repeated applications

Publication:
Stem Cells, 2013. doi.org/10.1002/stem.1495

Author(s):
Scott R. Burks, Ali Ziadloo, Saejeong J. Kim, et al.

Quansys Products Used:
16-Plex Mouse Cytokine Screen

Abstract:

Show Abstract (234 words)

Title:
Human Macrophage Regulation Via Interaction With Cardiac Adipose Tissue-Derived Mesenchymal Stromal Cells

Publication:
J CARDIOVASC PHARMACOL THER, 2013. doi.org/10.1177/1074248412453875

Author(s):
Shimrit Adutler-Lieber, Tammar Ben-Mordechai, Nili Naftali-Shani, et al.

Quansys Products Used:
Q-Plex Arrays

Abstract:

Show Abstract (212 words)

Title:
Autoimmune processes in the placentas of neural tube defect-affected pregnancies

Publication:
University of Texas Libraries, 2013.

Author(s):
Ana Maria Palacios

Quansys Products Used:
Q-Plex Array, Q-View Imager, Q-View Software

Abstract:

Show Abstract (260 words)

Title:
Novel inflammatory biomarkers of portal pressure in compensated cirrhotic patients

Publication:
Hepatology, 2013. doi.org/10.1002/hep.26755

Author(s):
Buck M, Garcia-Tsao G, Groszmann RJ, et al.

Quansys Products Used:
Human Cytokine IR and Q-View Imager

Abstract:

Show Abstract (236 words)

Title:
The Origin of Human Mesenchymal Stromal Cells Dictates Their Reparative Properties

Publication:
Journal of the American Heart Association. doi.org/10.1161/JAHA.113.000253

Author(s):
Nili Naftali‐Shani, Ayelet Itzhaki‐Alfia, Natalie Landa‐Rouben, et al.

Quansys Products Used:
Custom Human Q-Plex Array and Q-View Software

Abstract:

Show Abstract (209 words)

Title:
HIV DNA Reservoir Increases Risk for Cognitive Disorders in cART-Naïve Patients

Publication:
Plos One 8, 2013. doi.org/10.1371/journal.pone.0070164

Author(s):
Valcour VG, Ananworanich J, Agsalda M, Sailasuta N, Chalermchai T, et al.

Quansys Products Used:
Custom Multiplex ELISA array and Q-View Plus Software

Abstract:

Show Abstract (114 words)

Title:
Obstructive Sleep Apnea Is a Predictor of Abnormal Glucose Metabolism in Chronically Sleep Deprived Obese Adults

Publication:
Plos One 8, 2013. doi.org/10.1371/journal.pone.0065400

Author(s):
Cizza G, Piaggi P, Lucassen EA, et al.

Quansys Products Used:
Human 16-Plex Multiplex ELISA

Abstract:

Show Abstract (50 words)

Title:
Human Umbilical Cord Blood-Derived CD34+ Cells Reverse Osteoporosis in NOD/SCID Mice by Altering Osteoblastic and Osteoclastic Activities

Publication:
Plos One 7, 2013. doi.org/10.1371/journal.pone.0039365

Author(s):
Aggarwal R, Lu J, Kanji S, et al.

Quansys Products Used:
Sample Testing Services

Abstract:

Show Abstract (153 words)

Title:
Characterization of hematopoietic potential of mesenchymal stem cells

Publication:
Journal of Cellular Physiology, 2013. doi.org/10.1002/jcp.22299

Author(s):
Eva Freisinger, Christopher Cramer, Xiujin Xia, et al.

Quansys Products Used:
Q-View Plus Software

Abstract:

Show Abstract (170 words)

Title:
Pro-inflammatory cytokines in glaucomatous aqueous and encysted Molteno implant blebs, and their relationship to pressure

Publication:
Invest. Ophthalmol. Vis. Sci., 2013. doi.org/10.1167/iovs.13-12274

Author(s):
Jeffrey Freedman and Pavel Iserovich

Quansys Products Used:
Q-View Software

Abstract:

Show Abstract (182 words)

Title:
An Extraribosomal Function of Ribosomal Protein L13a in Macrophages Resolves Inflammation

Publication:
The Journal of Immunology, 2013. doi.org/10.4049/jimmunol.1201933

Author(s):
Darshana Poddar, Abhijit Basu, William M. Baldwin III, et al.

Quansys Products Used:
Sample Testing Services

Abstract:

Show Abstract (164 words)

Title:
A Peptide Antagonist of CD28 Signaling Attenuates Toxic Shock and Necrotizing Soft-Tissue Infection Induced by Streptococcus pyogenes

Publication:
J Infect Dis., 2013. doi.org/10.1093/infdis/jit104

Author(s):
Girish Ramachandran, Mohan E. Tulapurkar, Kristina M. Harris, et al.

Quansys Products Used:
Mouse Cytokine Immunoassay (9-plex)

Abstract:

Show Abstract (146 words)

2012 Publications

Title:
Validation of a new multiplex assay against individual immunoassays for the quantification of reproductive, stress and energetic hormones in urine specimens

Publication:
American Journal of Human Biology, 2012. doi.org/10.1002/ajhb.21229

Author(s):
Salvante K, Brindle E, McConnell D, et al.

Quansys Products Used:
Human Female Hormone (8-plex), Q-View Imager, Q-View Software

Abstract:

Show Abstract (207 words)

Title:
Lutein and β-Cryptoxanthin Inhibit Inflammatory Mediators in Human Chondrosarcoma Cells Induced with IL-1β

Publication:
Open Nutrition Journal, 2012.

Author(s):
Michael M. Di Filippo, Bridget D. Mathison, Jean Soon Park, et al.

Quansys Products Used:
Human Multiplex Screen

Abstract:

Show Abstract (300 words)

Osteoarthritis (OA) and rheumatoid arthritis (RA) are characterized by the activation of matrix-degrading proteinases and production of inflammatory cytokines and mediators by chondrocytes in articular cartilage. Several studies have shown that the carotenoids lutein (Lu) and β-cryptoxanthin (βCr) may be useful in downregulating factors involved in the progression of OA and RA Therefore, the possible protective effects of Lu and βCr against arthritis were studied using SW-1353 cells induced by interleukin-1β (IL-1β). Cells were cultured for 24 hr with 0, 0.01, 0.1 and 1.0 µmol/L of Lu (Experiment 1) or βCr (Experiment 2) and stressed by IL1β for 24 hr. Conditioned medium was analyzed for proteinases, inflammatory cytokines and mediators, and nuclear extract for NFκB. The addition of βCr had no effect on matrixmetalloproteinase-13 (MMP-13) production; however, 1.0 µmol/L Lu decreased matrix MMP-13 (P<0.02) and PGE2 (P<0.04) production. Similarly the addition of 0.1 and 0.01 µmol/L βCr also decreased (P<0.01) PGE2 production. At various concentrations (0.01, 0.1, and 1.0 µmol/L), βCr decreased the pro-inflammatory cytokines IL-1α (P<0.05), IL-2 (P<0.04), and IFN-γ. In contrast, Lu increased the IL-1α, IL-2 (P<0.01), and interferon (IFN)-γ. Lu increased the antiinflammatory cytokines IL-4 (P<0.02) and IL-10, while βCr decreased IL-4 and IL-10 (P<0.02). vNFκB p50 was decreased by Lu at all concentrations tested including a 39% reduction in cultures containing 0.1 µmol/L. The βCr also decreased concentrations of nuclear p50 by 38% in cells preincubated with 1.0 µmol/L. In conclusion, Lu and βCr protected against degenerative factors upregulated by IL-1β-stimulation, likely by scavenging reactive oxygen species required for NFκB activation. Chondrocytes cultured in the presence of Lu downregulated factors involved in cartilage destruction (MMP-13 and PGE2) and upregulated factors (IL-4) involved in tissueprotection, while addition of βCr resulted in strong downregulation of factors associated with pro-inflammatory response (IL-1α, IL-2, and IFN-γ) and cartilage destruction (PGE2).

Title:
Stress management skills, neuroimmune processes and fatigue levels in persons with chronic fatigue syndrome

Publication:
Brain, Behavior, and Immunity, 2012. doi.org/10.1016/j.bbi.2012.02.008

Author(s):
Lattie, Emily G., Antoni, Michael H.

Quansys Products Used:
Human Multiplex Screen, Q-View Imager, Q-View Software

Abstract:

Show Abstract (119 words)

Title:
Impact of Multi-Targeted Antiretroviral treatment on Gut T Cell Depletion and HIV Reservoir Seeding during Acute HIV Infection

Publication:
Plos One 7, 2012. doi.org/10.1371/journal.pone.0033948

Author(s):
Jintanat Ananworanich, Alexandra Schuetz, Claire Vandergeeten, et al.

Quansys Products Used:
Human Screen IR Multiplex (16-plex) and Q-View Software

Abstract:

Show Abstract (257 words)

Title:
Urban Particulate matter activates Akt in human lung cells

Publication:
Arch Toxicol, 2012. doi.org/10.1007/s00204-011-0739-5

Author(s):
Todd Watterson, Brett Hamilton, et al.

Quansys Products Used:
Human Multiplex ELISA

Abstract:

Title:
Immunological characterization of a gidA mutant strain of Salmonella for potential use in a live-attenuated vaccine

Publication:
BMC Microbiology, 2012. doi.org/10.1186/1471-2180-12-286

Author(s):
Daniel C. Shippy, Amin A. Fadl

Quansys Products Used:
Custom Multiplex Assay

Abstract:

Show Abstract (74 words)

Title:
Plasmodium falciparum merozoite surface protein 1 blocks the proinflammatory protein S100P

Publication:
PNAS, 2012. doi.org/10.1073/pnas.1202689109

Author(s):
Waisberg M, Cerqueira G, Yager S, et al.

Quansys Products Used:
Q-Plex Human Cytokine Kit

Abstract:

Show Abstract (118 words)

Title:
Validation of a new multiplex assay against individual immunoassays for the quantification of reproductive, stress, and energetic metabolism biomarkers in urine specimens

Publication:
American Journal of Human Biology, 2012. doi.org/10.1002/ajhb.21229

Author(s):
Salvante, K., E. Brindle, et al.

Quansys Products Used:
Quansys Female Hormone Multiplex (8-plex)

Abstract:

Show Abstract (127 words)

Title:
High-fat diet exacerbates MPTP-induced dopaminergic degeration in mice

Publication:
Neurobiology of Disease, 2012. doi.org/10.1016/j.nbd.2011.09.009

Author(s):
Bousquet, M., St-Amour, I., et al.

Quansys Products Used:
Mouse Multiplex Screen

Abstract:

Show Abstract (176 words)

Title:
Suppression of EAE and prevention of blood-brain barrier breakdown after vaccination with novel bicunctional peptide inhibitor

Publication:
Neuropharmacology, 2012. doi.org/10.1016/j.neuropharm.2011.12.013

Author(s):
Ahmed H. Badawi, Paul Kiptoo

Quansys Products Used:
Mouse Multiplex Screen

Abstract:

Show Abstract (198 words)

Title:
Multiplexing and multivariate analysis in neurodegeneration

Publication:
Methods, 2012. doi.org/10.1016/j.ymeth.2012.02.004

Author(s):
Andreasson U., Portelius E., Pannee J., et al.

Quansys Products Used:
Multiplex Kits

Abstract:

Show Abstract (88 words)

Title:
Hyaluronan expressed by the hematopoietic microenvironment is required for bone marrow hematopoiesis

Publication:
The Journal of Biological Chemistry, 2012. doi.org/10.1074/jbc.M112.376699

Author(s):
Goncharova V., Serobyan N., Iizuka S., et al.

Quansys Products Used:
Mouse Cytokine Antibody Array

Abstract:

Show Abstract (245 words)

Title:
Molecular and Cellular Characterization of a Salmonella enterica Serovar Paratyphi A Outbreak Strain and the Human Immune Response to its Infection

Publication:
Clinical and Vaccine Immunology, 2012. doi.org/10.1128/CVI.05468-11

Author(s):
Ohad Gal-Mor, Jotham Suez, Dana Elhadad, et al.

Quansys Products Used:
Human Multiplex Screen, Q-View Imager, Q-View Software

Abstract:

Show Abstract (254 words)

Title:
Damage Control Resuscitation Decreases Systemic Inflammation After Hemorrhage

Publication:
Journal of Surgical Research, 2012. doi.org/10.1016/j.jss.2011.11.1028

Author(s):
Makley, Amy T., Goodman, Michael D., et al.

Quansys Products Used:
Mouse Multiplex Screen

Abstract:

Show Abstract (119 words)

Title:
Impaired hepatic function and central dopaminergic denervation in a rodent model of Parkinson’s disease: A self-perpetuating crosstalk?

Publication:
Biochemica et Biophysica Acta, 2012. doi.org/10.1016/j.bbadis.2011.11.008

Author(s):
Vairetti, Mariapia., Ferrigno, Andrea., et al

Quansys Products Used:
Rat Cytokine Multiplex

Abstract:

Show Abstract (246 words)

Title:
A 4-trifluoromethyl analogue of celecoxib inhibits arthritis by suppressing innate immune cell activation

Publication:
Arthritis Research & Therapy, 2012. doi.org/10.1186/ar3683

Author(s):

Asako Chiba, Miho Mizuno, et al.

Quansys Products Used:
Human Cytokine Stripwells (16-Plex)

Abstract:

Show Abstract (160 words)

Title:
Ustekinumab demonstrates activity in glucocorticoid-refractory acute GVHD

Publication:
Bone Marrow Transplantation, 2012. doi.org/10.1038/bmt.2011.172

Author(s):
J. Pidala, L. Perez, F. Beato, et al.

Quansys Products Used:
Custom ELISA Assay

Abstract:

Show Abstract (221 words)

Title:
Poly-IC preconditioning protects against cerebral and renal ischemia-reperfusion injury

Publication:
Journal of Cerebral Blood Flow & Metabolism, 2012. doi.org/10.1038/jcbfm.2011.160

Author(s):
Amy E. B. Packard, Jason C. Hedges, Frances R. Bahjat, et al.

Quansys Products Used:
Multiplex ELISA for IL-1ß, IL-12, IFNγ

Abstract:

Show Abstract (96 words)

2011 Publications

Title:
Hypobaric hypoxia exacerbates the neuroinflammatory response to traumatic brain injury

Publication:
Journal of Surgical Research, 2011. doi.0rg/10.1016/j.jss.2010.05.055

Author(s):
M. Goodman, A. Makley, et al.

Quansys Products Used:
Mouse Cytokine Multiplex ELISA

Abstract:

Show Abstract (110 words)

Title:
Biological and virulence characteristics of Salmonella enterica serovar Typhimurium following deletion of glucose-inhibited division (gidA) gene

Publication:
Microbial Pathogenesis, 2011. doi.org/10.1016/j.micpath.2011.02.004

Author(s):
Shippy D.C., Eakley N.M., Bochsler P.N., et al.

Quansys Products Used:
Mouse Cytokine Multiplex ELISA

Abstract:

Show Abstract (198 words)

Title:
Oral TNF-a gene silencing using a polymeric microsphere-based delivery system for the treatment of inflammatory bowel disease

Publication:
Journal of Controlled Release, 2011. doi.org/10.1016/j.jconrel.2010.10.002

Author(s):
Kriegel C., Amiji M.

Quansys Products Used:
Mouse Cytokine Multiplex ELISA and Q-View Software

Abstract:

Show Abstract (150 words)

Title:
Multiplex measurement of proinflammatory cytokines in human serum: Comparison of the Meso Scale Discovery electrochemiluminescence assay and the Cytometric Bead Array

Publication:
Journal of Immunological Methods, 2011. doi.org/10.1016/j.jim.2011.06.033

Author(s):
Dabitao D., Margolick J.B., Lopez J., et al.

Quansys Products Used:
Human Cytokine Multiplex ELISA

Abstract:

Show Abstract (225 words)

Title:
a-1-Antitrypsin Gene Delivery Reduces Inflammation, Increases T-Regulatory Cell Population Size and Prevents Islet Allograft Rejection

Publication:
I Mol Med, 2011. doi.org/10.2119/molmed.2011.00145

Author(s):
Galit Shahaf, Hadas Moser, Eyal Ozeri, et al.

Quansys Products Used:
Mouse Cytokine Multiplex ELISA, Q-view Software, Q-view Imager

Abstract:

Show Abstract (284 words)

Antiinflammatory clinical-grade, plasma-derived human α-1 antitrypsin (hAAT) protects islets from allorejection as well as from autoimmune destruction. hAAT also interferes with disease progression in experimental autoimmune encephalomyelitis (EAE) and in collagen-induced arthritis (CIA) mouse models. hAAT increases IL-1 receptor antagonist expression in human mononuclear cells and T-regulatory (Treg) cell population size in animal models. Clinical-grade hAAT contains plasma impurities, multiple hAAT isoforms and various states of inactive hAAT. We thus wished to establish islet-protective activities and effect on Treg cells of plasmid-derived circulating hAAT in whole animals. Islet function was assessed in mice that received allogeneic islet transplants after mice were given hydrodynamic tail-vein injection with pEF-hAAT, a previously described Epstein-Barr virus (EBV) plasmid construct containing the EBV nuclear antigen 1 (EBNA1) and the family of repeat EBNA1 binding site components (designated “EF”) alongside the hAAT gene. Sera collected from hAAT-expressing mice were added to lipopolysaccharide (LPS)-stimulated macrophages to assess macrophage responsiveness. Also, maturation of peritoneal cells from hAAT-expressing mice was evaluated. hAAT-expressing mice accepted islet allografts (n = 11), whereas phosphate-buffered saline–injected animals (n = 11), as well as mice treated with truncated-hAAT-plasmid (n = 6) and untreated animals (n = 20) rapidly rejected islet allografts. In hAAT-expressing animals, local Treg cells were abundant at graft sites, and the IL-1 receptor antagonist was elevated in grafts and circulation. Sera from hAAT-expressing mice, but not control mice, inhibited macrophage responses. Finally, peritoneal cells from hAAT-expressing mice exhibited a semimature phenotype. We conclude that plasmid-derived circulating hAAT protects islet allografts from acute rejection, and human plasma impurities are unrelated to islet protection. Future studies may use this in vivo approach to examine the structure–function characteristics of the protective activities of AAT by manipulation of the hAAT plasmid.

Title:
Modulation of Chemokines and Allergic Airway Inflammation by Selective Local Sphingosine-1-phosphate Receptor 1 Agonism in Lungs

Publication:
Molecular Pharmacology, 2011. doi.org/10.1124/mol.110.066811

Author(s):
Marsolais D., Yagi S., Kago T., et al.

Quansys Products Used:
Mouse Cytokine Muliplex ELISA

Abstract:

Show Abstract (198 words)

Title:
Distinct cytokine and regulatory T cell profile

Publication:
Clinical and Experimental Immunology, 2011. doi.org/10.1111/j.1365-2249.2010.04269.x

Author(s):
Toossi Z., Hirsch C.S., Wu M., et al.

Quansys Products Used:
Human Cytokine Multiplex ELISA

Abstract:

Show Abstract (245 words)

Title:
Membrane-rich milk fat diet provides protection against gastrointestinal leakiness in mice treated with lipopolusaccharide

Publication:
J. Dairy Sci, 2011. doi.org/10.3168/jds.2010-3886

Author(s):

Snow D.R., Ward R.E., Olsen A., et al.

Quansys Products Used:
Mouse Cytokine Multiplex ELISA

Abstract:

Show Abstract (222 words)

Title:
Retinoic Acid Attenuates Ileitis by Restoring the Balance Between T-Helper 17 and T Regulatory Cells

Publication:
Gastroenterology, 2011. doi.org/10.1053/j.gastro.2011.05.049

Author(s):
Collins C.B., Aherne C.M., Kominsky D., et al.

Quansys Products Used:
Mouse Cytokine Multiplex ELISA and Q-View Software

Abstract:

Title:
Dual TNF-a/Cyclin D1 Gene Silencing With an Oral Polymeric Microparticle System as a Novel Strategy for the Treatment of Inflammatory Bowel Disease

Publication:
Clinical and Translational Gastroenterology, 2011. doi.org/10.1038/ctg.2011.1

Author(s):
Christina Kriegel, Mansoor M. Amiji

Quansys Products Used:
Mouse Cytokine Multiplex ELISA and Q-view Software

Abstract:

Show Abstract (130 words)

Title:
Cell Type Specificity and Host Genetic Polymorphisms influence Antibody-Dependent Enhancement of Dengue Virus Infection

Publication:
Journal of Virology, 2011. doi.org/10.1128/JVI.00220-10

Author(s):
Kobporn Boonnak, Kaitlyn M. Dambach, Gina C. Donofrio, et al.

Quansys Products Used:
Human Cytokine Infrared Multiplex ELISA

Abstract:

Show Abstract (255 words)

Title:
Investigation of Cellular and Molecular Responses to Pulsed Focused Ultrasound in a Mouse Model

Publication:
Focused Ultrasound-Induced Molecular Responses, 2011. doi.org/10.1371/journal.pone.0024730

Author(s):
Scott R. Burks, Ali Ziadloo, Hilary A. Hancock, et al.

Quansys Products Used:
Mouse Cytokine Multiplex ELISA

Abstract:

Show Abstract (234 words)

Title:
Ex vivo expansion of human Tregs specific for alloantigens presented directly or indirectly

Publication:
Blood, 2012. doi.org/10.1182/blood-2011-02-337097

Author(s):
Veerapathran A., Pidala J., Beato F., et al.

Quansys Products Used:
Human Cytokine Multiplex ELISA

Abstract:

Show Abstract (200 words)

Title:
Inhibition of severe acute respiratory syndrome coronavirus replication in a lethal SARS-CoV BALB/c mouse model by stinging nettle lectin

Publication:
Antiviral Research, 2012. doi.org/10.1016/j.antiviral.2011.02.003

Author(s):
Kumaki Y., Wandersee M.K., Smith A.J., et al.

Quansys Products Used:
Mouse Cytokine Multiplex ELISA

Abstract:

Show Abstract (365 words)

Urtica dioica agglutinin (UDA) is a small plant monomeric lectin, 8.7 kDa in size, with an N-acetylglucosamine specificity that inhibits viruses from Nidovirales in vitro. In the current study, we first examined the efficacy of UDA on the replication of different SARS-CoV strains in Vero 76 cells. UDA inhibited virus replication in a dose-dependent manner and reduced virus yields of the Urbani strain by 90% at 1.1 ± 0.4 μg/ml in Vero 76 cells. Then, UDA was tested for efficacy in a lethal SARS-CoV-infected BALB/c mouse model. BALB/c mice were infected with two LD50 (575 PFU) of virus for 4 h before the mice were treated intraperitoneally with UDA at 20, 10, 5 or 0 mg/kg/day for 4 days. Treatment with UDA at 5 mg/kg significantly protected the mice against a lethal infection with mouse-adapted SARS-CoV (p < 0.001), but did not significantly reduce virus lung titers. All virus-infected mice receiving UDA treatments were also significantly protected against weight loss (p < 0.001). UDA also effectively reduced lung pathology scores. At day 6 after virus exposure, all groups of mice receiving UDA had much lower lung weights than did the placebo-treated mice. Thus, our data suggest that UDA treatment of SARS infection in mice leads to a substantial therapeutic effect that protects mice against death and weight loss. Furthermore, the mode of action of UDA in vitro was further investigated using live SARS-CoV Urbani strain virus and retroviral particles pseudotyped with SARS-CoV spike (S). UDA specifically inhibited the replication of live SARS-CoV or SARS-CoV pseudotyped virus when added just before, but not after, adsorption. These data suggested that UDA likely inhibits SARS-CoV infection by targeting early stages of the replication cycle, namely, adsorption or penetration. In addition, we demonstrated that UDA neutralizes the virus infectivity, presumably by binding to the SARS-CoV spike (S) glycoprotein. Finally, the target molecule for the inhibition of virus replication was partially characterized. When UDA was exposed to N-acetylglucosamine and then UDA was added to cells just prior to adsorption, UDA did not inhibit the virus infection. These data support the conclusion that UDA might bind to N-acetylglucosamine-like residues present on the glycosylated envelope glycoproteins, thereby preventing virus attachment to cells.

Title:
Endothelial Cells Are Central Orchestrators of Cytokine Amplification during Influenza Virus Infection

Publication:
Cell, 2011. doi.org/10.1016/j.cell.2011.08.015

Author(s):
John R. Teijaro, Kevin B. Walsh, Stuart Cahalan, et al.

Quansys Products Used:
Mouse Cytokine Multiplex ELISA

Abstract:

Title:
Sustained expression of circulating human alpha-1 antitrypsin reduces inflammation, increases CD4+FoxP3+ Treg cell population and prevents signs of experimental autoimmune encephalomyelitis in mice

Publication:
Metab Brain Dis, 2011. doi.org/10.1007/s11011-011-9239-9

Author(s):
Subramanian S., Shahaf G., Ozeri E., et al.

Quansys Products Used:
Mouse Cytokine Multiplex ELISA and Q-View Imager

Abstract:

Show Abstract (134 words)

Title:
Mice with Different Susceptibility to Japanese Encephalitis Virus Infection Show Selective Neutralizing Antibody Response and Myeloid Cell Infectivity

Publication:
Plos One 6, 2011. doi.org/10.1371/journal.pone.0024744

Author(s):
Kai Wang, Vincent Deubel

Quansys Products Used:
Mouse Cytokine Multiplex ELISA and Q-view Software

Abstract:

Title:
Enhanced Transferrin Reseptor Expression by Proinflammatory Cytokines in Enterocytes as a Means for Local Delivery of Drugs to Inflamed Gut Mucosa

Publication:
Plos One 9, 2011. doi.org/10.1371/journal.pone.0024202

Author(s):
Efrat Harel, Abraham Rubinstein, Aviram Nissan, et al.

Quansys Products Used:
Mouse Cytokine Multiplex ELISA

Abstract:

Title:
TLR2 Activation Is Essential to Induce a Th1 Shift in Human Peripheral Blood Mononuclear Cells by Plant Stanols and Plant Sterols

2010 Publications

Publication:
J Biol Chem. 2010, doi: 10.1074/jbc.M109.036343

Author(s):
Florence Brüll, Ronald P. Mensink, Karin van den Hurk, et al.

Quansys Products Used:
Human Cytokine Screen (16-plex), Q-View Imager

Abstract:

Show Abstract (189 words)

Title:
Serum S100B: a potential biomarker for suicidality in adolescents?

Publication:
PLoS One, 2010. doi.org/10.1371/journal.pone.0011089

Author(s):
Falcone, T., V. Fazio, et al

Quansys Products Used:
Human Cytokine Multiplex ELISA

Abstract:

Show Abstract (101 words)

Title:
A CD36-dependent pathway enhances macrophage and adipose tissue inflammation and impairs insulin signalling

Publication:
Cardiovasc Res., 2010. doi.org/10.1093/cvr/cvq360

Author(s):
Kennedy, D. J., S. Kuchibhotla, et al

Quansys Products Used:
Mouse Cytokine Infrared Multiplex ELISA

Abstract:

Show Abstract (135 words)

Title:
Protein kinase C theta deficiency increases resistance of C57BL/6J mice to Plasmodium berghei infection-induced cerebral malaria

Publication:
Infect Immun, 2010. doi.org/10.1128/IAI.00465-10

Author(s):
Ohayon, A., J. Golenser, et al

Quansys Products Used:
Mouse Chemokine Multiplex ELISA

Abstract:

Show Abstract (247 words)

Title:
Profile of lipid and protein autacoids in diabetic vitreous correlates with the progression of diabetic retinopathy

Publication:
Diabetes, 2010. doi.org/10.2337/db10-0110

Author(s):
Schwartzman, M. L., P. Iserovich, et al

Quansys Products Used:
Custom Angiogenisis Multiplex ELISA

Abstract:

Title:
Genetic susceptibility to systemic lupus erythematosus protects against cerebral malaria in mice

Publication:
Proc Natl Acad Sci, 2010. doi.org/10.1073/pnas.1017996108

Author(s):
Waisberg, M., T. Tarasenko, et al

Quansys Products Used:
Human Cytokine Multiplex ELISA

Abstract:

Title:
A formal analysis of cytokine networks in chronic fatigue syndrome

Publication:
Brain Behav Immun, 2010. doi.org/10.1016/j.bbi.2010.04.012

Author(s):
Broderick, G., J. Fuite, et al

Quansys Products Used:
Human Cytokine Multiplex ELISA, Q-View Imager

Abstract:

Title:
Epigallocatechin gallate delays the onset of type 1 diabetes in spontaneous non-obese diabetic mice

Publication:
Br J Nutr, 2010. doi.org/10.1017/S0007114510004824

Author(s):
Fu, Z., W. Zhen, et al

Quansys Products Used:
Mouse Cytokine Multiplex ELISA

Abstract:

Show Abstract (236 words)

Title:
Resuscitation with fresh whole blood ameliorates the inflammatory response after hemorrhagic shock

Publication:
J Trauma, 2010. doi.org/10.1097/TA.0b013e3181cb4472

Author(s):
Makley, A. T., M. D. Goodman, et al

Quansys Products Used:
Mouse Cytokine Multiplex ELISA

Abstract:

Show Abstract (139 words)

Title:
Isolation and expansion of human natural T regulatory cells for cellular therapy

Publication:
J Immunol, 2010. doi.org/10.1016/j.jim.2010.10.006

Author(s):
Pahwa, R., S. Jaggaiahgari, et al

Quansys Products Used:
Human Cytokine Multiplex ELISA

Abstract:

Show Abstract (197 words)

Title:
Epithelial-derived IL-33 and its receptor ST2 are dysregulated in ulcerative colities and in experimental Th1/Th2 driven enteritis

Publication:
Proc Natl Acad Sci, 2010. doi.org/10.1073/pnas.0912678107

Author(s):
Pastorelli, L., R. Garg, et al

Quansys Products Used:
Human and Mouse Cytokine Multiplex ELISA

Abstract:

Show Abstract (230 words)

Title:
Low level exposure to monomethyl arsonous acid-induced the over-production of inflammation-related cytokines and the activation of cell signals associated with tumor progression in a urothelial cell model

Publication:
Toxicology and Applied Pharmacology, 2010. doi.org/10.1016/j.taap.2009.12.029

Author(s):
Escudero-Lourdes, C., M.K. Medeiros, et al

Quansys Products Used:
Human Cytokine Multiplex ELISA, Q-View Software

Abstract:

Show Abstract (226 words)

Title:
Ex vivo development, expansion and in vivo analysis of a novel lineage of dendritic cells from hematopoietic stem cells

Publication:
J Immune, 2010. doi.org/10.1186/1476-8518-8-8

Author(s):
Han, S., Y. Wang, et al

Quansys Products Used:
Sample Testing Services

Abstract:

Show Abstract (240 words)

Title:
Effect of dipterinyl calcium pentahydrate on hepatitis B virus replication in transgenic mice

Publication:
J Transl Med, 2010. doi.org/10.1186/1479-5876-8-32

Author(s):
Moheno, P., J. Morrey, et al

Quansys Products Used:
Mouse Chemokine Multiplex ELISA

Abstract:

Show Abstract (114 words)

Title:
Elevated IL-17 produced by TH17 cells promotes myeloma cell growth and inhibits immune function in multiple myeloma

Publication:
Blood, 2010. doi.org/10.1182/blood-2009-10-246660

Author(s):
Prabhala, R. H., D. Pelluru, et al.

Quansys Products Used:
Human Cytokine Multiplex ELISA

Abstract:

Show Abstract (206 words)

Title:
Simultaneous measurement of multiple ear proteins with multiplex ELISA assays

Publication:
Hear Res., 2010. doi.org/10.1016/j.heares.2010.11.009

Author(s):
Trune, D. R., B. E. Larrain, et al.

Quansys Products Used:
Sample Testing Services

Abstract:

Show Abstract (326 words)

A recent advancement in enzyme-linked immunosorbent assay (ELISA) technology is the multiplex antibody array that measures multiple proteins simultaneously within a single sample. This allows reduction in sample volume, time, labor, and material costs, while increasing sensitivity over single ELISA. Current multiplex platforms include planar-based systems using microplates or slides, or bead-based suspension assay with microspheres. To determine the applicability of this technology for ear research, we used 3 different multiplex ELISA-based immunoassay arrays from 4 different companies to measure cytokine levels in mouse middle and inner ear tissue lysate extracts 24 hours following transtympanic Haemophilus influenzae inoculation. Middle and inner ear tissue lysates were analyzed using testing services from Quansys BioSciences, Aushon Biosystems SearchLight (both microplate-based), Milliplex MAP Sample (bead-based), and a RayBiotech, Inc. (slide-based) kit. Samples were assayed in duplicate or triplicate. Results were compared to determine their relative sensitivity and reliability for measures of cytokine related to inflammation. The cytokine pg/ml amounts varied among the multiplex assays, so a comparison also was made of the mean fold increase in cytokines from untreated controls. Several cytokines and chemokines were elevated, the extent dependent upon the assay sensitivity. Those most significantly elevated were IL-1α, IL-1β, IL-6, TNFα, VEGF, IL-8/MIP-2. The results of the multiplex systems were compared with single ELISA kits (IL-1β, IL-6) to assess sensitivity over the traditional method. Overall, the Quansys Biosciences and SearchLight arrays showed the greatest sensitivity, both employing the same multiplex methodology of a spotted array within a microplate well with chemiluminescent detection. They also were more sensitive than the traditional single ELISA performed with commercial kits and matched gene expression changes determined by quantitative RT-PCR. The Quansys array showed a limit of detection for ear IL-6 down to 2–4 pg/ml, indicating it is sufficiently sensitive to detect ear proteins present in low concentrations. Thus, the multiplex ELISA procedures appear suitable and reliable for the study of hearing related proteins, providing accurate, quantitative, reproducible results with considerable improvement in sensitivity and economy.

2009 Publications

Title:
Increased Cytokine Production in Interleukin-18 Receptor α-deficient Cells Is Associated with Dysregulation of Suppressors of Cytokine Signaling

Publication:
J Biol Chem., 2009. doi.org/10.1074/jbc.M109.004184

Author(s):
Claudia A. Nold-Petry, Marcel F. Nold, Jason W. Nielsen, et al.

Quansys Products Used:
Q-View Imager

Abstract:

Show Abstract (175 words)

Title:
Plasma cytokines in women with chronic fatigue syndrome

Publication:
J Transl Med, 2009. doi.org/10.1186/1479-5876-7-96

Author(s):
Fletcher, M. A., X. R. Zeng, et al

Quansys Products Used:
Human Cytokine, Imager

Abstract:

Title:
Increased cytokine production in interleukin-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling

Publication:
J Biol Chem, 2009. doi.org/10.1074/jbc.M109.004184

Author(s):
Nold-Petry, C. A., M. F. Nold, et al

Quansys Products Used:
Q-view Imager

Abstract:

Show Abstract (219 words)

Title:
Meprin A metalloproteases enhance renal damage and bladder inflammation after LPS challenge

Publication:
Am J Physiol Renal Physiol. doi.org/10.1152/ajprenal.90524.2008

Author(s):
Yura, R. E., S. G. Bradley, et al.

Quansys Products Used:
Mouse Cytokine, Q-View Software

Abstract:

Title:
MEP1A allele for meprin A metalloprotease is a susceptibility gene for inflammatory bowel disease

Publication:
Mucosal Immunol, 2009. doi.org/10.1038/mi.2009.3

Author(s):
Banerjee, S., B. Oneda, et al

Quansys Products Used:
Q-View Software

Abstract:

Show Abstract (141 words)

Title:
Combined transductional untargeting/retargeting and transcriptional restriction enhances adenovirus gene targeting and therapy for hepatic colorectal cancer tumors

Publication:
Cancer Res, 2009. doi.org/10.1158/0008-5472.CAN-08-3209

Author(s):
Li, H. J., M. Everts, et al

Quansys Products Used:
Mouse Cytokine

Abstract:

Show Abstract (251 words)

Title:
Stimulatory effects of CpG oligodeoxynucleotide on dendritic cell-based immunotherapy of colon cancer in CEA/HLA-A2 transgenic mice

Publication:
Int J Cancer, 2009. doi.org/10.1002/ijc.24009

Author(s):
Saha, A., M. Bhattacharya-Chatterjee, et al

Quansys Products Used:
Mouse Cytokine

Abstract:

Show Abstract (210 words)

Title:
The roles of chemokines in rabies virus infection: overexpression may not always be beneficial

Publication:
J Virol, 2009. doi.org/10.1128/JVI.01346-09

Author(s):
Zhao, L., H. Toriumi, et al

Quansys Products Used:
Mouse Cytokine

Abstract:

Show Abstract (186 words)

Title:
A new mouse-adapted strain of SARS-CoV as a lethal model for evaluating antiviral agents in vitro and in vivo

Publication:
Virology, 2009. doi.org/10.1016/j.virol.2009.09.023

Author(s):
Day, C. W., R. Baric, et al

Quansys Products Used:
Mouse Cytokine, Q-View Software

Abstract:

Show Abstract (142 words)

Title:
Second-hand cigarette smoke inhibits wound healing of the cornea by stimulating inflammation that delays corneal reepithelialization

Publication:
Wound Repair Regen, 2009. doi.org/10.1111/j.1524-475X.2009.00478.x

Author(s):
Ma, C. and M. Martins-Green

Quansys Products Used:
Mouse Cytokine

Abstract:

Show Abstract (194 words)

Title:
Lentiviral calnexin-modified dendritic cells promote expansion of high-avidity effector T cells with central memory phenotype

Publication:
Immunology, 2009. doi.org/10.1111/j.1365-2567.2009.03067.x

Author(s):
Wang, B., S. Han, et al

Quansys Products Used:
Sample Testing Service

Abstract:

Show Abstract (172 words)

2008 Publications

Title:
Is the anti-psychotic, 10-(3-(dimethylamino)propyl)phenothiazine (promazine), a potential drug with which to treat SARS infections? Lack of efficacy of promazine on SARS-CoV replication in a mouse model

Publication:
Antiviral Res, 2008. doi.org/10.1016/j.antiviral.2007.12.005

Author(s):
Barnard, D., C. Day, et al.

Quansys Products Used:
Mouse Cytokine, Q-View Software

Abstract:

Show Abstract (175 words)

Title:
ELISA and multiplex technologies for cytokine measurement in inflammation and aging research

Publication:
J Gerontol A Biol Sci Med Sci., 2008.

Author(s):
Leng, S., J.E. McElhaney, et al

Quansys Products Used:
Multiplex ELISA

Abstract:

Title:
Thrombospondin 1 promotes tumor macrophage recruitment and enhances tumor cell cytotoxicity of differentiated U937 cells

Publication:
Cancer Res, 2008. doi.org/10.1158/0008-5472.CAN-08-0643

Author(s):
Martin-Manso, G., S. Galli, et al

Quansys Products Used:
Mouse Cytokine

Abstract:

Show Abstract (134 words)

Title:
Multiplex immunoassay for serological diagnosis of Mycobacterium bovis infection in cattle

Publication:
Clin Vaccine Immunol, 2008. doi.org/10.1128/CVI.00238-08

Author(s):
Whelan, C., E. Shuralev, et al

Quansys Products Used:
Custom Multiplex, Q-View Imager, Q-View Software

Abstract:

Title:
C3H/HeN mouse model for the evaluation of antiviral agents for the treatment of Venezuelan equine encephalitis virus infection

Publication:
Antiviral Res, 2008. doi.org/10.1016/j.antiviral.2008.01.007

Author(s):
Ulander, J. G., R. Skirpstunas, et al

Quansys Products Used:
Mouse Cytokine

Abstract:

Show Abstract (200 words)

Title:
Antibody protein array analysis of the tear film cytokines

Publication:
Optom Vis Sci, 2008. doi.org/10.1097/OPX.0b013e3181824e20

Author(s):
Li, S., R. Sack, et al

Quansys Products Used:
Human Cytokine

Abstract:

Show Abstract (156 words)

Title:
Efficacy of cationic lipid-DNA complexes (CLDC) on hepatitis B virus in transgenic mice

Publication:
Antiviral Res, 2008. doi.org/10.1016/j.antiviral.2008.01.157

Author(s):
Morrey, J. D., N. E. Motter, et al

Quansys Products Used:
Mouse Cytokine, Q-View Software

Abstract:

Show Abstract (197 words)

Title:
A novel inhibitor of signal transducers and activators of transcription 3 activation is efficacious against established central nervous system melanoma and inhibits regulatory T cells

Publication:
Clin Cancer Res, 2008. doi.org/10.1158/1078-0432.CCR-08-0377

Author(s):
Kong, L. Y., M. K. Abou-Ghazal, et al

Quansys Products Used:
Sample Testing Services

Abstract:

Show Abstract (215 words)

Title:
Local not systemic modulation of dendritic cell S1P receptors in lung blunts virus-specific immune responses to influenza

Publication:
Mol Pharmacol, 2008. doi.org/10.1124/mol.108.048769

Author(s):
Marsolais, D., B. Hahm, et al

Quansys Products Used:
Human Cytokine, Sample Testing

Abstract:

Show Abstract (206 words)

Title:
Differential pathogenesis of cowpox virus intranasal infections in mice induced by low and high inoculum volumes and effects of cidofovir treatment

Publication:
Int J Antimicrob Agents, 2008. doi.org/10.1016/j.ijantimicag.2007.11.013

Author(s):
Smee, D. F., B. B. Gowen, et al

Quansys Products Used:
Mouse Cytokine

Abstract:

Title:
Enterococcal leucine-rich repeat-containing protein involved in virulence and host inflammatory response

2007 Publications

Publication:
Infect Immun, 2007. doi.org/10.1128/IAI.00279-07

Author(s):
Brinster, S., B. Posteraro, et al

Quansys Products Used:
Mouse Cytokine, Q-View Software

Abstract:

Show Abstract (165 words)

Title:
A 22-plex chemiluminescent microarray for pneumococcal antibodies

Publication:
Am J Clin Pathol, 2007. doi.org/10.1309/781K5W6QH7JH2TMA

Author(s):
Pickering, J. W., J. D. Hoopes, et al

Quansys Products Used:
Human Cytokine and Q-View Software

Abstract:

Show Abstract (174 words)

Title:
Cellular and functional characterization of immunoresistant human glioma cell clones selected with alloreactive cytotoxic T lymphocytes reveals their up-regulated synthesis of biologically active TGF-beta

Publication:
J Immunother, 2007. doi.org/10.1097/01.cji.0000211339.81211.25

Author(s):
Gomez, G. G. and C. A. Kruse

Quansys Products Used:
Human Cytokine and Q-View Softyware

Abstract:

Show Abstract (118 words)

Title:
The effects of second-hand smoke on biological processes important in atherogenesis

Publication:
BMC Cardiovasc Disord, 2007. doi.org/10.1186/1471-2261-7-1

Author(s):
Yuan, H., L. S. Wong, et al

Quansys Products Used:
Mouse Cytokine and Q-View Software

Abstract:

Title:
Validating a custom multiplex ELISA against individual commercial immunoassays using clinical samples

Publication:
Biotechniques, 2007.

Author(s):
Liew, M., M. C. Groll, et al

Quansys Products Used:
Multiplex ELISA

Abstract:

Show Abstract (221 words)

Title:
Molecular mechanisms of thrombin-induced interleukin-8 (IL-8/CXCL8) expression in THP-1-derived and primary human macrophages

Publication:
J Leukoc Biol, 2007. doi.org/10.1189/jlb.0107009

Author(s):
Zheng, L. and M. Martins-Green

Quansys Products Used:
Human Cytokine and Q-View Software

Abstract:

Show Abstract (186 words)

2006 Publications

Title:
Enhancement of the infectivity of SARS-CoV in BALB/c mice by IMP dehydrogenase inhibitors, including ribavirin

Publication:
Antiviral Res, 2006. doi.org/10.1016/j.antiviral.2006.03.001

Author(s):
Barnard, D. L., C. W. Day, et al

Quansys Products Used:
Mouse Cytokine

Abstract:

Show Abstract (308 words)

Because of the conflicting data concerning the SARS-CoV inhibitory efficacy of ribavirin, an inosine monophosphate (IMP) dehydrogenase inhibitor, studies were done to evaluate the efficacy of ribavirin and other IMP dehydrogenase inhibitors (5-ethynyl-1-beta-D-ribofuranosylimidazole-4-carboxamide (EICAR), mizoribine, and mycophenolic acid) in preventing viral replication in the lungs of BALB/c mice, a replication model for severe acute respiratory syndrome (SARS) infections (Subbarao, K., McAuliffe, J., Vogel, L., Fahle, G., Fischer, S., Tatti, K., Packard, M., Shieh, W.J., Zaki, S., Murphy, B., 2004. Prior infection and passive transfer of neutralizing antibody prevent replication of severe acute respiratory syndrome coronavirus (SARS-CoV) in the respiratory tract of mice. J. Virol. 78, 3572-3577). Ribavirin given at 75 mg/kg 4 h prior to virus exposure and then given twice daily for 3 days beginning at day 0 was found to increase virus lung titers and extend the length of time that virus could be detected in the lungs of mice. Other IMP dehydrogenase inhibitors administered near maximum tolerated doses using the same dosing regimen as for ribavirin were found to slightly enhance virus replication in the lungs. In addition, ribavirin treatment seemed also to promote the production of pro-inflammatory cytokines 4 days after cessation of treatment, although after 3 days of treatment ribavirin inhibited pro-inflammatory cytokine production in infected mice, significantly reducing the levels of the cytokines IL-1alpha, interleukin-5 (IL-5), monocyte chemotactic protein-1 (MCP-1), and granulocyte-macrophage colony stimulating factor (GM-CSF). These findings suggest that ribavirin may actually contribute to the pathogenesis of SARS-CoV by prolonging and/or enhancing viral replication in the lungs. By not inhibiting viral replication in the lungs of infected mice, ribavirin treatment may have provided a continual source of stimulation for the inflammatory response thought to contribute to the pathogenesis of the infection. Our data do not support the use of ribavirin or other IMP dehydrogenase inhibitors for treating SARS infections in humans.

Title:
TLR3 deletion limits mortality and disease severity due to Phlebovirus infection

Publication:
J Immunol, 2006.

Author(s):
Gowen, B. B., J. D. Hoopes, et al

Quansys Products Used:
Mouse Cytokine

Abstract: