Storage of Q‐Plex Kit Components for Long‐term Studies

Using a freshly reconstituted Calibrator vial for each experiment is essential to obtaining consistent results over time. Let us know at the time of your initial purchase if you plan to utilize a portion of a Q‐Plex plate on a different day, so we can provide extra Calibrator vials from a single production Lot for each experiment, as well as extra plate seals. Our Stripwell Kits are manufactured specifically for this purpose, and so each kit comes with three calibrator vials from the same Lot, and extra plate seals. DO NOT use calibrator solution that has been frozen, as even a single freeze‐thaw cycle can have a significantly deleterious effect. Also, keep microplate wells to be used later dry; do not wash them along with the in‐use portion of the plate.

PartDescriptionReagent PreparationStorage of Opened Material
Q-Plex Array MicroplateSpotted and blocked 96-well polystyrene microtiter plateReady for use.If the plate contains un-wetted wells to be used later, return the plate to 4°C in its sealed foil pouch. Good until kit expiration.
Wash Buffer Concentrate (20X)Liquid, 50 mL/vial of a concentrated solution of buffered surfactantPlace 50 mL of the 20X concentrate into 950 mL deionized water, mix thoroughly.4°C until kit expiration.
CalibratorLyophilized, recombinant calibrators in a buffered protein base with preservatives, see the Product Card for high pointsWhen ready to begin the assay, reconstitute in Sample Diluent according to the Product Card which accompanies the kit, mix gently until fully reconstituted.Differences Between ELISA Kits ExplainedDiscard unused reconstituted calibrator. Do not freeze/thaw. Good for 6 hrs on ice.*
Sample DiluentLiquid, 6 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers, and preservativesReady for use.4°C until kit expiration
Detection MixLiquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservativesReady for use.4°C until kit expiration
Streptavidin-HRP 1XLiquid, 6 mL/vial of streptavidin-conjugated horse radish peroxidaseReady for use. Do not expose to UV light.Do not expose to UV light. 4°C until kit expiration.
Substrate ALiquid, 3 mL/vial of stabilized hydrogen peroxideDo not expose to UV light. Do not cool after mixing. During the assay, mix 3 mL of Substrate A with 3 mL of Substrate B, and mix gently.Do not expose to UV light. Do not cool after mixing. Store mixed substrate solution at room temperature for up to 1 week. Store unmixed solution at 4°C until kit expiration.
Substrate BLiquid, 3 mL/vial of stabilized signal enhancerDo not expose to UV light. Do not cool after mixing. During the assay, mix 3 mL of Substrate A with 3 mL of Substrate B, and mix gently.Do not expose to UV light. Do not cool after mixing. Store mixed substrate solution at room temperature for up to 1 week. Store unmixed solution at 4°C until kit expiration.
Plate Seals (3)Adhesive stripsNoneNon-perishable

*DO NOT re-use reconstituted calibrator solution that has been frozen, as even a single freeze-thaw cycle will have a deleterious effect on the calibrators.