How do your multiplex ELISA kits work?

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How do your multiplex ELISA kits work?

The Q-Plex kits consist of a printed array of capture antibodies for a variety of biological markers. The user adds a calibrator and samples to individual wells of a 96-well plate. After incubation and washing, a mixture of biotinylated antibodies is added to the plate. This is followed by incubating with streptavidin-HRP or IR dye and is completed by capturing the light produced from adding chemiluminescent substrate with an imaging system. Pixel intensity values from the images are used to construct a standard curve and calculate the concentrations of the unknown sample for each system.

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