How should I dilute my sample if I expect a high response for one biomarker and a low response for another?

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How should I dilute my sample if I expect a high response for one biomarker and a low response for another?

We recommend testing each sample in step dilutions. For example, when testing cell culture supernatant for unknown cytokine concentrations; a 1:2, a 1:10, and a 1:50 dilution are typically used.
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