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Q-Plex™ Human Pneumococcal IgG (23-Plex)

Q-Plex Human Pneumococcal IgG (23-Plex)

List of Serotypes: 1, 2, 3, 4, 5, 6B, 7F, 8, 9N, 9V, 10A, 11A, 12F, 14, 15B, 17F, 18C, 19A, 19F, 20, 22F, 23F, 33F 

  • Species Specificity: Human
  • Validated Sample Type: Serum
  • Sample Volume Required: 0.25 µL
  • Estimated Assay Length: 3 hours
  • Intra-Assay Cross Reactivity: High levels of 19F may inhibit the detection of 12F. No cross-reactivity or interference detected between other analytes. 
  • Assay Type: Indirect
  • Detection Method: Chemiluminescent
  • Multiplex Format: 96-well solid plate
  • Catalog 23-Plex: #481149HU
    • Catalog 14-Plex: #481749HU
    • Catalog 9-Plex: #481649HU

Q-Plex Human Pneumococcal IgG (23-Plex)

Serotypes: 1, 2, 3, 4, 5, 6B, 7F, 8, 9N, 9V, 10A, 11A, 12F, 14, 15B, 17F, 18C, 19A, 19F, 20, 22F, 23F, 33F

This kit is a chemiluminescent multiplex ELISA run on two 96-well plates, one plate will run 14 serotypes (1, 3, 4, 5, 6B, 7F, 8, 9N, 9V, 12F, 14, 18C, 19F, 23F) and the other plate will run the remaining 9 serotypes (2, 10A, 11A, 15B, 17F, 19A, 20, 22F, 33F).

Q-Plex Human Pneumococcal IgG (14-Plex)

Serotypes: 1, 3, 4, 5, 6B, 7F, 8, 9N, 9V, 12F, 14, 18C, 19F, 23F

This kit can be ordered individually from the 23-Plex. 

Q-Plex Human Pneumococcal IgG (9-Plex)

Serotypes: 2, 10A, 11A, 15B, 17F, 19A, 20, 22F, 33F

This kit can be ordered individually from the 23-Plex. 

Q-Plex™ Human Pneumococcal IgG (14-Plex)

Serotype Assay Type Calibrator Range Upper Limit of
Quantification (ULOQ)
Lower Limit of
Quantification (LLOQ)
Limit of Detection Precision* (Inter-assay) Precision* (Intra-assay) Average Linearity
1 Indirect 9 - 0.012 (µg/mL) 5.95 (µg/mL) 0.015 (µg/mL) 0.008 (µg/mL) 14.1% 7.7% 92-112% (100%)
3 Indirect 1 - 0.004 (µg/mL) 1.02 (µg/mL) 0.023 (µg/mL) 0.012 (µg/mL) 20.0% 10.5% 93-120% (103%)
4 Indirect 3 - 0.004 (µg/mL) 2.33 (µg/mL) 0.018 (µg/mL) 0.009 (µg/mL) 12.2% 7.8% 89-106% (100%)
5 Indirect 8 - 0.011 (µg/mL) 5.26 (µg/mL) 0.013 (µg/mL) 0.007 (µg/mL) 14.1% 9.8% 100-111% (106%)
6B Indirect 9 - 0.012 (µg/mL) 6.34 (µg/mL) 0.016 (µg/mL) 0.008 (µg/mL) 11.1% 11.2% 93-109% (100%)
7F Indirect 9 - 0.012 (µg/mL) 5.81 (µg/mL) 0.044 (µg/mL) 0.022 (µg/mL) 14.2% 10.2% 91-117% (103%)
8 Indirect 14 - 0.019 (µg/mL) 9.97 (µg/mL) 0.229 (µg/mL) 0.0114 (µg/mL) 12.1% 8.3% 95-123% (106%)
9N Indirect 7 - 0.01 (µg/mL) 4.92 (µg/mL) 0.038 (µg/mL) 0.019 (µg/mL) 12.7% 9.9% 98-106% (101%)
9V Indirect 7 - 0.01 (µg/mL) 4.51 (µg/mL) 0.034 (µg/mL) 0.017 (µg/mL) 11.5% 7.7% 85-108% (97%)
12F Indirect 2 - 0.025 (µg/mL) 1.55 (µg/mL) 0.035 (µg/mL) 0.018 (µg/mL) 20.7% 8.3% 97-128% (115%)
14 Indirect 38 - 0.052 (µg/mL) 8.86 (µg/mL) 0.068 (µg/mL) 0.034 (µg/mL) 13.1% 9.9% 94-123% (104%)
18C Indirect 7 - 0.01 (µg/mL) 5.11 (µg/mL) 0.039 (µg/mL) 0.02 (µg/mL) 11.6% 9.2% 91-106% (101%)
19F Indirect 15 - 0.021 (µg/mL) 10.23 (µg/mL) 0.026 (µg/mL) 0.013 (µg/mL) 11.8% 8.3% 90-112% (100%)
23F Indirect 6 - 0.025 (µg/mL) 4.17 (µg/mL) 0.032 (µg/mL) 0.016 (µg/mL) 15.5% 10.4% 100-119% (107%)

*Intra-assay and inter-assay precision are calculated with n=20 and n=10 replicates, respectively.

Q-Plex™ Human Pneumococcal IgG (9-Plex)

Serotype Assay Type Calibrator Range Upper Limit of
Quantification (ULOQ)
Lower Limit of
Quantification (LLOQ)
Limit of Detection Precision* (Inter-assay) Precision* (Intra-assay) Average Linearity
2 Indirect 25 - 0.034 (µg/mL) 17.24 (µg/mL) 0.044 (µg/mL) 0.022 (µg/mL) 18.4% 10.9% 88-513% (181%)
10A Indirect 13 - 0.018 (µg/mL) 9.09 (µg/mL) 0.069 (µg/mL) 0.035 (µg/mL) 13.7% 9.5% 91-106% (99%)
11A Indirect 5 - 0.007 (µg/mL) 1.19 (µg/mL) 0.027 (µg/mL) 0.014 (µg/mL) 13.5% 9.9% 91-104% (97%)
15B Indirect 17 - 0.023 (µg/mL) 11.86 (µg/mL) 0.03 (µg/mL) 0.015 (µg/mL) 11.5% 9.7% 86-102% (98%)
17F Indirect 9 - 0.111 (µg/mL) 5.96 (µg/mL) 0.137 (µg/mL) 0.068 (µg/mL) 15.9% 12.3% 86-109% (100%)
19A Indirect 14 - 0.058 (µg/mL) 9.71 (µg/mL) 0.074 (µg/mL) 0.037 (µg/mL) 14.5% 9.0% 95-104% (100%)
20 Indirect 11 - 0.045 (µg/mL) 7.33 (µg/mL) 0.019 (µg/mL) 0.009 (µg/mL) 11.7% 9.2% 93-102% (99%)
22F Indirect 10 - 0.014 (µg/mL) 6.65 (µg/mL) 0.051 (µg/mL) 0.025 (µg/mL) 17.7% 10.1% 94-119% (102%)
33F Indirect 11 - 0.015 (µg/mL) 2.49 (µg/mL) 0.019 (µg/mL) 0.01 (µg/mL) 12.6% 9.4% 94-101% (98%)

*Intra-assay and inter-assay precision are calculated with n=20 and n=10 replicates, respectively.

Background

The bacterium Streptococcus pneumoniae can cause potentially deadly infections in children and the elderly, including pneumonia and meningitis. Risk of infection and disease severity varies with age of the patient and a host of other factors including geographical location, ethnic background, and time of year. More than 90 serotypes are known, though less than 25 are responsible for most cases, with virulence often differing between serotypes.

Principle of the Assay

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Antibodies play an essential role in the protection against pneumococcal disease. In humans, serum antibodies typically act against the polysaccharide capsule in a serotype specific manner. There are multiple vaccination options for protection against S. pneumoniae, with the major difference being the number and composition of specific serotypes present in the vaccine.

The Q-Plex™ Human Pneumococcal IgG (23-Plex) is a chemiluminescent multiplex ELISA run on two 96-well plates comprising the serotypes found in the Pneumovax®23 vaccine.

A 14-Plex plate detects 14 of the common serotypes individuals are vaccinated against while the second detects 9 additional serotypes commonly used as vaccination targets. Limits for these assays were set using the 007sp Human Anti-Pneumococcal capsule Reference Serum.

The Q-Plex™ Human Pneumococcal IgG (14-Plex) and Q-Plex™ Human Pneumococcal IgG (9-Plex) are also available for individual purchase.

Kit Content

Each kit contains a 96-well plate, featuring the relevant biomarker panel in each well, and all reagents required to perform testing.

Reagents Include:

  • Detection Mix
  • Calibrators
  • Substrates
  • Sample Diluents
  • Streptavidin HRP
  • Wash Buffer

Processing

All Quansys Q-Plex™ Multiplex and Singleplex assays require the use of the Q-View™ Software to read and interpret the test results. A fully-functional, free trial version of the software is available to download, install, and use to analyze your first kit(s). At the end of the trial period, a purchased license is required to continue the use of the Q-View software.

Q-Plex™ Arrays are developed and optimized to work with Q-View™ Imagers. Quansys does not guarantee the results obtained from other imagers and not all imagers are compatible with Quansys Q-Plex Arrays.

Other Equipment Recommendations

Not Finding What You Need?

Customize a Kit

Not seeing the biomarker or analyte you need? Customize your kit! The Q-Plex™ Custom Assays are fully quantitative ELISA-based Chemiluminescent assay kits allowing the concurrent measurement of up to 18 biomarkers or analytes.

Send in Your Samples

Choose up to 18 different biomarkers that will work best for your research. Send in your samples and let us run them for you. 

Contact a Representative

Have questions about the kit or interested in more information? Contact one of our representative to get all your questions answered. 

As researchers and problem solvers ourselves, we understand the value of sound data. We are proud to be a part of research that can better the world. We value the relationships, partnerships, and friendships that we have built with the people who trust and use our technology. We are committed to building these relationships. You can count on us to answer the phone and take time to thoroughly address questions or concerns about any of our products. In an industry that is reputed for grandiose claims, we trust that our quality standards and our customer service set us apart from the competition. If you’re happy with our products or if you think we can do something better, we hope you will let us know.

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