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Q-Plex™ Human Pneumococcal IgG (23-Plex)

Pneumococcal 14-Plex Transparent
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Q-Plex Human Pneumococcal IgG (23-Plex)

List of Serotypes: 1, 2, 3, 4, 5, 6B, 7F, 8, 9N, 9V, 10A, 11A, 12F, 14, 15B, 17F, 18C, 19A, 19F, 20, 22F, 23F, 33F 

  • Species Specificity: Human
  • Validated Sample Type: Serum
  • Sample Volume Required: 0.25 µL
  • Estimated Assay Length: Less than 1.25 hours
  • Intra-Assay Cross Reactivity: No significant interference observed. <1% cross-reactivity observed. 
  • Assay Type: Indirect
  • Detection Method: Chemiluminescent
  • Multiplex Format: 96-well solid plate
  • Catalog 23-Plex: #481149HU
    • Catalog 14-Plex: #481749HU
    • Catalog 9-Plex: #481649HU

Q-Plex Human Pneumococcal IgG (23-Plex)

Serotypes: 1, 2, 3, 4, 5, 6B, 7F, 8, 9N, 9V, 10A, 11A, 12F, 14, 15B, 17F, 18C, 19A, 19F, 20, 22F, 23F, 33F

This kit is a chemiluminescent multiplex ELISA run on two 96-well plates, one plate will run 14 serotypes (1, 3, 4, 5, 6B, 7F, 8, 9N, 9V, 12F, 14, 18C, 19F, 23F) and the other plate will run the remaining 9 serotypes (2, 10A, 11A, 15B, 17F, 19A, 20, 22F, 33F).

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Q-Plex Human Pneumococcal IgG (14-Plex)

Serotypes: 1, 3, 4, 5, 6B, 7F, 8, 9N, 9V, 12F, 14, 18C, 19F, 23F

This kit can be ordered individually from the 23-Plex.

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Q-Plex Human Pneumococcal IgG (9-Plex)

Serotypes: 2, 10A, 11A, 15B, 17F, 19A, 20, 22F, 33F

This kit can be ordered individually from the 23-Plex.

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Q-Plex™ Human Pneumococcal IgG (14-Plex)

Serotype Assay Type Calibrator Range Upper Limit ofQuantification (ULOQ) Lower Limit ofQuantification (LLOQ) Limit of Detection Precision* (Inter-assay) Precision* (Intra-assay) Average Linearity
1 Indirect 0.85 - 0.00117 (μg/mL) 0.259 (μg/mL) 0.000468 (μg/mL) 0.000583 (μg/mL) 6% 7% 97%
3 Indirect 0.0145 - 0.00020 (μg/mL) 0.294 (μg/mL) 0.000533 (μg/mL) 9.95E-05 (μg/mL) 12% 14% 99%
4 Indirect 0.33 - 0.00045 (μg/mL) 0.154 (μg/mL) 0.000273 (μg/mL) 0.000226 (μg/mL) 5% 6% 99%
5 Indirect 0.75 - 0.00103 (μg/mL) 0.371 (μg/mL) 0.000676 (μg/mL) 0.000514 (μg/mL) 8% 8% 102%
6B Indirect 0.91 - 0.00125 (μg/mL) 0.133 (μg/mL) 0.000247 (μg/mL) 0.000624 (μg/mL) 8% 9% 103%
7F Indirect 0.83 - 0.00114 (μg/mL) 0.392 (μg/mL) 0.00286 (μg/mL) 0.000569 (μg/mL) 6% 7% 100%
8 Indirect 1.42 - 0.00195 (μg/mL) 0.133 (μg/mL) 0.000962 (μg/mL) 0.000974 (μg/mL) 7% 8% 97%
9N Indirect 0.87 - 0.00119 (μg/mL) 0.154 (μg/mL) 0.001105 (μg/mL) 0.000597 (μg/mL) 8% 10% 102%
9V Indirect 0.64 - 0.00088 (μg/mL) 0.203 (μg/mL) 0.00143 (μg/mL) 0.000439 (μg/mL) 8% 8% 102%
12F Indirect 0.22 - 0.00030 (μg/mL) 0.07 (μg/mL) 0.00052 (μg/mL) 0.000151 (μg/mL) 11% 9% 93%
14 Indirect 3.8 - 0.00521 (μg/mL) 0.119 (μg/mL) 0.000871 (μg/mL) 0.002606 (μg/mL) 7% 7% 98%
18C Indirect 0.73 - 0.00100 (μg/mL) 0.217 (μg/mL) 0.00156 (μg/mL) 0.000501 (μg/mL) 5% 5% 100%
19F Indirect 1.46 - 0.00200 (μg/mL) 0.924 (μg/mL) 0.00676 (μg/mL) 0.001001 (μg/mL) 9% 12% 100%
23F Indirect 0.6 - 0.00082 (μg/mL) 0.084 (μg/mL) 0.000624 (μg/mL) 0.000412 (μg/mL) 10% 10% 99%
*Intra-assay and inter-assay precision are calculated with n=20 and n=10 replicates, respectively.

Q-Plex™ Human Pneumococcal IgG (9-Plex)

Serotype Assay Type Calibrator Range Upper Limit ofQuantification (ULOQ) Lower Limit ofQuantification (LLOQ) Limit of Detection Precision* (Inter-assay) Precision* (Intra-assay) Average Linearity
2 Indirect 2.46 - 0.00337 (μg/mL) 2.44 (μg/mL) 0.00061 (μg/mL) 0.00169 (μg/mL) 10% 12% 101%
10A Indirect 1.29 - 0.00177 (μg/mL) 0.3 (μg/mL) 0.00032 (μg/mL) 0.000885 (μg/mL) 7% 8% 106%
11A Indirect 0.508 - 0.00070 (μg/mL) 0.14 (μg/mL) 0.00013 (μg/mL) 0.000348 (μg/mL) 7% 8% 103%
15B Indirect 1.69 - 0.00232 (μg/mL) 0.47 (μg/mL) 0.00044 (μg/mL) 0.001159 9% 10% 105%
17F Indirect 0.85 - 0.00117 (μg/mL) 0.79 (μg/mL) 0.00067 (μg/mL) 0.000583 (μg/mL) 9% 8% 98%
19A Indirect 1.39 - 0.00191 (μg/mL) 1.24 (μg/mL) 0.0061 (μg/mL) 0.000953 (μg/mL) 6% 6% 101%
20 Indirect 1.05 - 0.00144 (μg/mL) 0.26 (μg/mL) 0.00028 (μg/mL) 0.00072 (μg/mL) 6% 6% 102%
22F Indirect 0.95 - 0.00130 (μg/mL) 0.95 (μg/mL) 0.00094 (μg/mL) 0.000652 (μg/mL) 9% 12% 107%
33F Indirect 1.07 - 0.00147 (μg/mL) 0.27 (μg/mL) 0.00026 (μg/mL) 0.000734 (μg/mL) 6% 6% 101%
*Intra-assay and inter-assay precision are calculated with n=20 and n=10 replicates, respectively.

Background

The bacterium Streptococcus pneumoniae can cause potentially deadly infections in children and the elderly, including pneumonia and meningitis. Risk of infection and disease severity varies with age of the patient and a host of other factors including geographical location, ethnic background, and time of year. More than 90 serotypes are known, though less than 25 are responsible for most cases, with virulence often differing between serotypes.

Principle of the Assay

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Antibodies play an essential role in the protection against pneumococcal disease. In humans, serum antibodies typically act against the polysaccharide capsule in a serotype specific manner. There are multiple vaccination options for protection against S. pneumoniae, with the major difference being the number and composition of specific serotypes present in the vaccine.

The Q-Plex™ Human Pneumococcal IgG (23-Plex) is a chemiluminescent multiplex ELISA run on two 96-well plates comprising the serotypes found in the Pneumovax®23 vaccine.

A 14-Plex plate detects 14 of the common serotypes individuals are vaccinated against while the second detects 9 additional serotypes commonly used as vaccination targets. Limits for these assays were set using the 007sp Human Anti-Pneumococcal capsule Reference Serum.

The Q-Plex™ Human Pneumococcal IgG (14-Plex) and Q-Plex™ Human Pneumococcal IgG (9-Plex) are also available for individual purchase.

Product Information

download Product Information Sheet

download SDS Information

download Q-Plex™ Human Pneumococcal IgG (23-Plex) Instruction Manual

Kit Content

Each kit contains a 96-well plate, featuring the relevant biomarker panel in each well, and all reagents required to perform testing.

Reagents Include:

  • Detection Mix
  • Calibrators
  • Substrates
  • Sample Diluents
  • Streptavidin HRP
  • Wash Buffer

Processing

All Quansys Q-Plex™ Multiplex and Singleplex assays require the use of the Q-View™ Software to read and interpret the test results. A fully-functional, free trial version of the software is available to download, install, and use to analyze your first kit(s). At the end of the trial period, a purchased license is required to continue the use of the Q-View software.

Q-Plex™ Arrays are developed and optimized to work with Q-View™ Imagers. Quansys does not guarantee the results obtained from other imagers and not all imagers are compatible with Quansys Q-Plex Arrays.

Other Equipment Recommendations

Publication Library

Search our publication library for all the peer-reviewed journals and research products that have utilized Q-Plex™ technology here.

In-Kit Certificate of Analysis

Search and download Certificates of Analysis for your Quansys Biosciences kits here.

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