Human GROα Analyte Overview

Human GROa

Also known as GROα, GRO alpha, CXCL1, KC, CINC-1, MGSA-alpha, Chemokine Ligand 1, Neutrophil-activating protein 3, NAP-3, GRO1 oncogene, Melanoma Growth Stimulating Activity alpha

Growth regulated α protein (GROα, GRO alpha) is a proinflammatory chemokine that plays a role in immune responses and many cancers. In the healthy immune system, GROα is produced by monocytes, epithelial cells, and fibroblasts in response to inflammatory activity. It induces neutrophil migration thought chemotaxis and has antimicrobial effects. It is also capable of promoting lymphatic tube and blood vessel formation within the circulatory system. In individuals with chronic inflammatory diseases, it is produced in high levels by many cell types. Abnormally high levels of GROα stimulates the growth of tumors and is particularly associated with melanomas due to its normal expression in epithelial cells. As a frequent oncogenic culprit, it is often found up-regulated when tumor cells are present throughout the body.

GROα Q-Plex™ details

Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 2,000 – 2.74 pg/ml
Lower Limit of Detection (LLD): 1.20 pg/ml
Volume Required Per Well: Min 25µl
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog No: 464249HU

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Measure GROα in 2.25 hours

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Sensitive, reproducible, and convenient 96-well format requiring low sample volumes

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Requires the Q-View™ Imaging System for analysis

GROα Assay Performance Characteristics

Precision

Intra-assay (n=20)
Average Intra-assay CV: 9%

Sample Concentration (pg/ml) %CV
Sample 1 368.24 10%
Sample 2 25.47 9%
Sample 3 8.60 7%

 

Inter-assay (n=15)
Average Inter-assay CV: 8%

Sample Concentration (pg/ml) %CV
Sample 1 719.47 6%
Sample 2 51.27 8%
Sample 3 9.97 10%

 

Dilutional Linearity

Average percent linearity: 101%
Percent linearity range: 89 – 108%

Dilution Factor % Recovery
Stripped Serum Cell Culture Supernatant Pooled Serum Serum HA
2 100% 106% 100% 107%
4 99% 102% 108% 102%
8 105% 105% 89% 94%
Human GROa Linearity

Q-Plex Multiplexed Products featuring GROα

Q-Plex Products featuring GROα

Human Multiplex Kits

Human Chemokine (9-plex)

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