Human I-309 Analyte Overview

Human I-309

Also known as CCL1, P500, SCYA1, T lymphocyte-secreted protein I-309, TCA-3

I-309, also known as CCL1, is a CC chemokine secreted by T-lymphocytes, monocytes and mast cells. As part of the inflammatory response, it is a potent chemoattractant to monocytes and Th2 differentiated T-cells. It also inhibits programmed cell death in thymus cells. I-309 has been shown to induce angiogenesis in vivo within rabbit cornea cultures and in chick chorioallantoic membrane assays. During early development, it binds to umbilical vein endothelial cells and induces their movement, invasion, and differentiation. These findings suggest that 1-309 may be necessary for building blood vessel walls. I-309’s receptor, CCR8, is a common target for HIV to enter cells. The presence of 1-309 inhibits HIV infection via CCR8.

I-309 Q-Plex™ details

Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 2,273 – 3.12 pg/ml
Lower Limit of Detection (LLD): 3.11 pg/ml
Volume Required Per Well: Min 25µl
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog No: 464549HU

N

Measure I-309 in 2.25 hours

N

Sensitive, reproducible, and convenient 96-well format requiring low sample volumes

N

Requires the Q-View™ Imaging System for analysis

I-309 Assay Performance Characteristics

Precision

Intra-assay (n=20)
Average Intra-assay CV: 9%

Sample Concentration (pg/ml) %CV
Sample 1

662.75

11%
Sample 2 35.50 12%
Sample 3 316.09 5%

 

Inter-assay (n=15)
Average Inter-assay CV: 10%

Sample Concentration (pg/ml) %CV
Sample 1 527.98 8%
Sample 2 513.20 8%
Sample 3 41.88 13%

 

Dilutional Linearity

Average percent linearity: 98%
Percent linearity range: 87 – 109%

Dilution Factor % Recovery
Serum HH Serum HG Serum HE Serum HF
2 191% 87% 94% 95%
4 101% 96% 99% 99%
8 109% 104% 100% 102%
human I-309 linearity

Q-Plex Multiplexed Products featuring I-309

Q-Plex Products featuring I-309

Human Multiplex Kits

Human Chemokine (9-plex)

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