Human TNFβ Analyte Overview
Human TNFb
Also known as TNFβ, Tumor Necrosis Factor Beta, Lymphotoxin-alpha, LT-α

TNFβ Q-Plex™ details
Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 4,000 – 5.49 pg/ml
Lower Limit of Detection (LLD): 5.29 pg/ml
Volume Required Per Well: Min 25µl
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog No: 469849HU
Measure TNFβ in 2.25 hours
Sensitive, reproducible, and convenient 96-well format requiring low sample volumes
Requires the Q-View™ Imaging System for analysis
TNFα Assay Performance Characteristics
Precision
Intra-assay (n=20)
Average Intra-assay CV: 7%
Sample | Concentration (pg/ml) | %CV |
Sample 1 | 782.91 | 5% |
Sample 2 | 68.66 | 6% |
Sample 3 | 21.47 | 9% |
Inter-assay (n=15)
Average Inter-assay CV: 8%
Sample | Concentration (pg/ml) | %CV |
Sample 1 | 596.30 | 8% |
Sample 2 | 33.15 | 10% |
Sample 3 | 871.31 | 6% |
Dilutional Linearity
Average percent linearity: 96%
Percent linearity range: 87 – 104%
Dilution Factor | % Recovery | |||
Cell Culture Supernatant | ||||
2 | 87% | |||
4 | 96% | |||
8 | 104% |

Q-Plex Multiplexed Products featuring TNFβ
Q-Plex Products featuring TNFβ
Human Multiplex Kits
Human Cytokine – Screen (16-plex)
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