Human Ang-2 Analyte Overview

Human Ang-2

Also known as Angiopoietin-2, ANGPT2

Angiopoietin2 (Ang-2) is a glycoprotein that is involved in the growth of new blood vessels and inflammation. Ang-2 is expressed heavily during early development, but in healthy adults is typically only expressed in the uterus and ovaries of females and during wound healing and inflammatory responses. During pregnancy Irregular levels of Ang-2 in the mother are associated with cases of pre-eclampsia, ectopic implantation, and Down Syndrome. For this reason, Ang-2 is often measured to predict at-risk-pregnancy. Ang-2 is implicated in cancer, during which the protein restricts blood vessels in expanding tumors, causing the regression and loss of vessels at the center of a tumor. Patients with a fever that are also expressing high levels of Ang-2 are at greater risk for the development of septic shock.

Ang-2 Q-Plex™ Details

Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma, Heparin
Assay Range: 10,000 – 13.70 pg/mL
Lower Limit of Detection (LLD): 3.9 pg/mL
Volume Required Per Well: Min. 25µL
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog #: 462349HU

Features & Requirements

Measure Ang-2 in 2.25 hours

Sensitive, reproducible, and convenient 96-well format requiring low sample volumes

Requires the Q-View™ Imaging System for analysis

Assay Performance Characteristics


Sample Concentration (pg/ml) %CV
Sample 1 1976.76 5%
Sample 2 13%
Sample 3 57.25 4%

Intra-assay (n=20)
Average Intra-assay CV: 7% 

Sample Concentration (pg/ml) %CV
Sample 1 781.95 9%
Sample 2 931.09 8%
Sample 3 1834.96 9%

Inter-assay (n=15)
Average Inter-assay CV: 9%

Dilutional Linearity

Dilution Factor % Recovery
Serum HA Serum HH Serum HD Pooled Plasma
2 100% 93% 92% 80%
4 103% 103% 90% 90%
8 108% 103% 95% 88%

Average percent linearity: 97%
Percent linearity range: 88 – 111%

Q-Plex Products Featuring Ang-2

Q-Plex™ Human Angiogenesis (9-Plex)

The Q-Plex Human Angiogenesis (9-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of Ang-2, FGF, HGF, IL-8, PDGF-BB, TIMP-1, TIMP-2, TNFα, and VEGF.

Not Finding What You Need?

Customize a Kit

Not seeing the biomarker or analyte you need? Customize your kit! The Q-Plex™ Custom Assays are fully quantitative ELISA-based Chemiluminescent assay kits allowing the concurrent measurement of up to 18 biomarkers or analytes.

Send in Your Samples

Choose up to 18 different biomarkers that will work best for your research. Send in your samples and let us run them for you. 

Contact a Representative

Have questions about the kit or interested in more information? Contact one of our representative to get all your questions answered. 

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