fbpx

Human Eotaxin Analyte Overview

Human Eotaxin

Also known as Eosinophil Chemotactic Protein, Chemokine ligand 11 (CCL11)

Eotaxins are a group of three cytokines, eotaxin-1, eotaxin-2, and eotaxin-3, that share similar structure and function. Eotaxins chemoattract eosinophils, which they are capable of activating and regulating during an immune response. Eotaxins have the unique ability to selectively prime eosinophils for chemotaxis, direct their migration/chemotaxis, and activate inflammatory activity in the cells attracted. Due to their role in attracting and activating eosinophils, eotaxins are implicated in allergies and eczema. High levels of eotaxin-1 are associated with aging and schizophrenia. Eotaxin-1 is also often found upregulated in cannabis users. Eotaxin-2 is also a chemoattractant for T lymphocytes and neutrophils. Its concentration is associated with risk and severity of asthma symptoms.

Q-Plex™ Details

Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 1,745 – 2.40 pg/mL
Lower Limit of Detection (LLD): 0.73 pg/mL
Volume Required Per Well: Min. 25µL
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog #: 463449HU

Features & Requirements

  • Measure Eotaxin in 2.25 hours
  • Sensitive, reproducible, and convenient 96-well format requiring low sample volumes
  • Requires the Q-View™ Imaging System for analysis

Assay Performance Characteristics

Precision

Sample Concentration (pg/ml) %CV
Sample 1 462.94 7%
Sample 2 29.68 7%
Sample 3 11.79 7%

Intra-assay (n=20)
Average Intra-assay CV: 7%

Sample Concentration (pg/ml) %CV
Sample 1 104.45 7%
Sample 2 896.61 5%
Sample 3 35.92 11%

Inter-assay (n=15)
Average Inter-assay CV: 8% 

Dilutional Linearity

Dilution Factor % Recovery
Serum HD Pooled Plasma Pooled Serum Stripped Serum
2 116%
4 109% 116% 115% 120%
8 89% 101% 100% 101%

Average percent linearity: 107%
Percent linearity range: 89 – 120%

Human eotaxin linearity

Featured Q-Plex Products 

Q-Plex™ Human Chemokine (9-Plex)

The Q-Plex Human Chemokine (9-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of Eotaxin, GROa, I-309, IL-8, IP-10, MCP-1, MCP-2, RANTES, and TARC.

Not Finding What You Need?

Customize a Kit

Not seeing the biomarker or analyte you need? Customize your kit! The Q-Plex™ Custom Assays are fully quantitative ELISA-based Chemiluminescent assay kits allowing the concurrent measurement of up to 18 biomarkers or analytes.

Send in Your Samples

Choose up to 18 different biomarkers that will work best for your research. Send in your samples and let us run them for you. 

Contact a Representative

Have questions about the kit or interested in more information? Contact one of our representative to get all your questions answered. 

As researchers and problem solvers ourselves, we understand the value of sound data. We are proud to be a part of research that can better the world. We value the relationships, partnerships, and friendships that we have built with the people who trust and use our technology. We are committed to building these relationships. You can count on us to answer the phone and take time to thoroughly address questions or concerns about any of our products. In an industry that is reputed for grandiose claims, we trust that our quality standards and our customer service set us apart from the competition. If you’re happy with our products or if you think we can do something better, we hope you will let us know.