Human I-309 Analyte Overview

Human I-309

Also known as CCL1, P500, SCYA1, T lymphocyte-secreted protein I-309, TCA-3

I-309, also known as CCL1, is a CC chemokine secreted by T-lymphocytes, monocytes and mast cells. As part of the inflammatory response, it is a potent chemoattractant to monocytes and Th2 differentiated T-cells. It also inhibits programmed cell death in thymus cells. I-309 has been shown to induce angiogenesis in vivo within rabbit cornea cultures and in chick chorioallantoic membrane assays. During early development, it binds to umbilical vein endothelial cells and induces their movement, invasion, and differentiation. These findings suggest that 1-309 may be necessary for building blood vessel walls. I-309’s receptor, CCR8, is a common target for HIV to enter cells. The presence of 1-309 inhibits HIV infection via CCR8.

Q-Plex™ Details

Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 2,273 – 3.12 pg/mL
Lower Limit of Detection (LLD): 3.11 pg/mL
Volume Required Per Well: Min. 25µL
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog #: 464549HU

Features & Requirements

  • Measures I-309 in 2.25 hours
  • Sensitive, reproducible, and convenient 96-well format requiring low sample volumes
  • Requires the Q-View™ Imaging System for analysis

Assay Performance Characteristics


Sample Concentration (pg/ml) %CV
Sample 1 662.75 11%
Sample 2 35.50 12%
Sample 3 316.09 5%

Intra-assay (n=20)
Average Intra-assay CV: 9%

Sample Concentration (pg/ml) %CV
Sample 1 527.98 8%
Sample 2 513.20 8%
Sample 3 41.88 13%

Inter-assay (n=15)
Average Inter-assay CV: 10%

Dilutional Linearity

Dilution Factor % Recovery
Serum HH Serum HG Serum HE Serum HF
2 191% 87% 94% 95%
4 101% 96% 99% 99%
8 109% 104% 100% 102%

Average percent linearity: 98%
Percent linearity range: 87 – 109%

human I-309 linearity

Featured Q-Plex Products 

Q-Plex™ Human Chemokine (9-Plex)

The Q-Plex Human Chemokine (9-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of Eotaxin, GROa, I-309, IL-8, IP-10, MCP-1, MCP-2, RANTES, and TARC.

Not Finding What You Need?

Customize a Kit

Not seeing the biomarker or analyte you need? Customize your kit! The Q-Plex™ Custom Assays are fully quantitative ELISA-based Chemiluminescent assay kits allowing the concurrent measurement of up to 18 biomarkers or analytes.

Send in Your Samples

Choose up to 18 different biomarkers that will work best for your research. Send in your samples and let us run them for you. 

Contact a Representative

Have questions about the kit or interested in more information? Contact one of our representative to get all your questions answered. 

As researchers and problem solvers ourselves, we understand the value of sound data. We are proud to be a part of research that can better the world. We value the relationships, partnerships, and friendships that we have built with the people who trust and use our technology. We are committed to building these relationships. You can count on us to answer the phone and take time to thoroughly address questions or concerns about any of our products. In an industry that is reputed for grandiose claims, we trust that our quality standards and our customer service set us apart from the competition. If you’re happy with our products or if you think we can do something better, we hope you will let us know.