Human MCP-2 Analyte Overview

Human MCP-2

Also known as Monocyte Chemotactic Protein-2, Chemokine Ligand 8, CCL8

Monocyte chemoattractant protein 2 (MCP-2), also known as Chemokine (C-C motif) ligand 8 (CCL8), is a chemokine involved in early stages of an immune responses. It interacts with cellular receptors CCR1, CCR2B, and CCR5 to attract and activate many types of immune cells, including monocytes, T-cells, and natural killer cells during and immune response and mast cells, eosinophils, and basophils in the case of an allergic response. MCP-2 is often expressed by stromal tissues that have been exposed to pathogens. It recruits immature monocytes to sites of inflammation to mature. Since MCP-2 demonstrates high affinity binding to receptor CCR5, a common HIV co-receptor, it has the potential to be HIV protective. It is often found in high levels in patients experiencing metastasis of a tumor and may play a role in promoting metastasis.

Q-Plex™ Details

Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 940 – 1.29 pg/mL
Lower Limit of Detection (LLD): 1.27 pg/mL
Volume Required Per Well: Min. 25µL
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog #: 467149HU

Features & Requirements

  • Measure MCP-2 in 2.25 hours
  • Sensitive, reproducible, and convenient 96-well format requiring low sample volumes
  • Requires the Q-View™ Imaging System for analysis

Assay Performance Characteristics


Intra-assay (n=20)
Average Intra-assay CV: 6%

Sample Concentration (pg/ml) %CV
Sample 1


Sample 2 14.23 6%
Sample 3 5.19 5%


Inter-assay (n=15)
Average Inter-assay CV: 11%

Sample Concentration (pg/ml) %CV
Sample 1 116.59 11%
Sample 2 60.53 13%
Sample 3 111.93 9%


Dilutional Linearity

Average percent linearity: 103%
Percent linearity range: 79 – 122%

Dilution Factor % Recovery
Cell Culture Supernatant Activated Plasma Pooled Serum Serum HF
2 98% 111% 110% 101%
4 105% 89% 111% 107%
8 101% 79% 98% 122%
human MCP-2 linearity

Featured Q-Plex Products

Human Multiplex Kits

Not Finding What You Need?

Customize a Kit

Not seeing the biomarker or analyte you need? Customize your kit! The Q-Plex™ Custom Assays are fully quantitative ELISA-based Chemiluminescent assay kits allowing the concurrent measurement of up to 18 biomarkers or analytes.

Send in Your Samples

Choose up to 18 different biomarkers that will work best for your research. Send in your samples and let us run them for you. 

Contact a Representative

Have questions about the kit or interested in more information? Contact one of our representative to get all your questions answered. 

As researchers and problem solvers ourselves, we understand the value of sound data. We are proud to be a part of research that can better the world. We value the relationships, partnerships, and friendships that we have built with the people who trust and use our technology. We are committed to building these relationships. You can count on us to answer the phone and take time to thoroughly address questions or concerns about any of our products. In an industry that is reputed for grandiose claims, we trust that our quality standards and our customer service set us apart from the competition. If you’re happy with our products or if you think we can do something better, we hope you will let us know.