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Human TARC Analyte Overview

Human TARC

Also known as CCL17, Thymus and Activation Regulated Chemokine, Chemokine Ligand 17

Thymus and activation regulated chemokine (TARC), also known as Chemokine (C-C motif) ligand 17 (CCL17), is a small cytokine involved in immune function of the thymus. TARC regulates T cell development in the thymus and directs the activation and mobilization of mature T cells. High levels of TARC in the blood are common during an allergic reaction. The cytokine is expressed by neurons in the hippocampus, however its role in the brain is not fully understood. Abnormal TARC expression is associated with a variety of diseases, including systemic lupus erythematosus, multiple sclerosis, atopic dermatitis, allergic rhinitis, and esophageal squamous cell carcinoma.

Q-Plex™ Details

Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 1,500 – 2.06 pg/mL
Lower Limit of Detection (LLD): 1.80 pg/mL
Volume Required Per Well: Min. 25µL
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog #: 469449HU

Features & Requirements

  • Measure TARC in 2.25 hours
  • Sensitive, reproducible, and convenient 96-well format requiring low sample volumes
  • Requires the Q-View™ Imaging System for analysis

Assay Performance Characteristics

Precision

Sample Concentration (pg/ml) %CV
Sample 1 248.37 7%
Sample 2 19.44 7%
Sample 3 7.31 9%

Intra-assay (n=20)
Average Intra-assay CV: 8%

Sample Concentration (pg/ml) %CV
Sample 1 69.22 9%
Sample 2 120.29 9%
Sample 3 165.45 6%

Inter-assay (n=15)
Average Inter-assay CV: 8%

Dilutional Linearity

Dilution Factor % Recovery
Cell Culture Supernatant Serum HB Serum HG Activated Plasma
2 111% 112% 102% 114%
4 103% 102% 109% 110%
8 107% 91% 101% 107%

Average percent linearity: 106%
Percent linearity range: 91 – 114%

Human TARC Linearity

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