Human Adiponectin Analyte Overview

Human Adiponectin

Also known as GBP-28, apM1, AdipoQ, Acrp30

Adiponectin is a protein hormone generated by adipose tissue that is involved in glucose regulation and fatty acid breakdown. Though the hormone is produced by fat cells, its levels are reduced in obese individuals and increase with caloric restriction and weight reduction. In lean individuals, the adiponectin is produced by adipose tissue in bone marrow. Decreased adiponectin levels are associated with metabolic disorders, including non-alcoholic fatty liver disease, obesity, type 2 diabetes, insulin resistance, and atherosclerosis, as well as some cancers. Studies suggest that the hormone promotes the synthesis of HDL cholesterol and low levels of adiponectin are associated with high triglyceride counts. Treatment with adiponectin has been demonstrated to reverse insulin resistance in mice and administration of a combination of leptin and adiponectin has been shown to trigger weight loss.

Adiponectin Q-Plex™ details

Species: Human
Assay Type: Competitive
Sample Type: Human Serum, EDTA Plasma
Assay Range: 28,000 – 38 ng/ml
Lower Limit of Detection (LLD): 31.5 ng/ml
Volume Required Per Well: Min 25µl
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog No: 462149HU

    N

    Measure Adiponectin in 2.25 hours

    N

    Sensitive, reproducible, and convenient 96-well format requiring low sample volumes

    N

    Requires the Q-View™ Imaging System for analysis

    Adiponectin Assay Performance Characteristics

    Precision

    Intra-assay (n=20)
    Average Intra-assay CV: 6%

    Sample Concentration (pg/ml) %CV
    Sample 1 38212.00 5%
    Sample 2 1964.95 7%
    Sample 3 564.35 7%

     

    Inter-assay (n=15)
    Average Inter-assay CV: 10%

    Sample Concentration (pg/ml) %CV
    Sample 1 496.6 15%
    Sample 2 11750.5 6%
    Sample 3 18620.2 7%

     

    Dilutional Linearity

    Average percent linearity: 97%
    Percent linearity range: 88 – 111%

    Dilution Factor % Recovery
    Serum 1 Serum 2 EDTA Plasma 1 EDTA Plasma 2
    2 105% 109% 100% 90%
    4 105% 111% 92% 89%
    8 97% 93% 91% 88%

    Q-Plex Multiplexed Products featuring Adiponectin

    Q-Plex Products featuring Adiponectin

    Human Multiplex Kits

    Human Female Hormone (8-plex)

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