Human Ang-2 Analyte Overview

Human Ang-2

Also known as Angiopoietin-2, ANGPT2

Angiopoietin2 (Ang-2) is a glycoprotein that is involved in the growth of new blood vessels and inflammation. Ang-2 is expressed heavily during early development, but in healthy adults is typically only expressed in the uterus and ovaries of females and during wound healing and inflammatory responses. During pregnancy Irregular levels of Ang-2 in the mother are associated with cases of pre-eclampsia, ectopic implantation, and Down Syndrome. For this reason, Ang-2 is often measured to predict at-risk-pregnancy. Ang-2 is implicated in cancer, during which the protein restricts blood vessels in expanding tumors, causing the regression and loss of vessels at the center of a tumor. Patients with a fever that are also expressing high levels of Ang-2 are at greater risk for the development of septic shock.

Ang-2 Assay Performance Characteristics

Ang-2 Q-Plex™ details

Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma, Heparin Plasma
Assay Range: 10,000 – 13.70 pg/ml
Lower Limit of Detection (LLD): 3.9 pg/ml
Volume Required Per Well: Min 25µl
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog No: 462349HU

    N

    Measure Ang-2 in 2.25 hours

    N

    Sensitive, reproducible, and convenient 96-well format requiring low sample volumes

    N

    Requires the Q-View™ Imaging System for analysis

    Precision

    Intra-assay (n=20)
    Average Intra-assay CV: 7%

    Sample Concentration (pg/ml) %CV
    Sample 1 1976.76 5%
    Sample 2 13%
    Sample 3 57.25 4%

     

    Inter-assay (n=15)
    Average Inter-assay CV: 9%

    Sample Concentration (pg/ml) %CV
    Sample 1 781.95 9%
    Sample 2 931.09 8%
    Sample 3 1834.96 9%

     

    Dilutional Linearity

    Average percent linearity: 97%
    Percent linearity range: 88 – 111%

    Dilution Factor % Recovery
    Serum HA Serum HH Serum HD Pooled Plasma
    2 100% 93% 92% 80%
    4 103% 103% 90% 90%
    8 108% 103% 95% 88%

    Q-Plex Multiplexed Products featuring Ang-2

    Q-Plex Products featuring Ang-2

    Human Multiplex Kits

    Human Angiogenesis (9-plex)

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