Human Eotaxin Analyte Overview

Human Eotaxin

Also known as Eosinophil Chemotactic Protein, Chemokine ligand 11 (CCL11)

Eotaxins are a group of three cytokines, eotaxin-1, eotaxin-2, and eotaxin-3, that share similar structure and function. Eotaxins chemoattract eosinophils, which they are capable of activating and regulating during an immune response. Eotaxins have the unique ability to selectively prime eosinophils for chemotaxis, direct their migration/chemotaxis, and activate inflammatory activity in the cells attracted. Due to their role in attracting and activating eosinophils, eotaxins are implicated in allergies and eczema. High levels of eotaxin-1 are associated with aging and schizophrenia. Eotaxin-1 is also often found upregulated in cannabis users. Eotaxin-2 is also a chemoattractant for T lymphocytes and neutrophils. Its concentration is associated with risk and severity of asthma symptoms.

Eotaxin Q-Plex™ details

Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 1,745 – 2.40 pg/ml
Lower Limit of Detection (LLD): 0.73 pg/ml
Volume Required Per Well: Min 25µl
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog No: 463449HU

N

Measure Eotaxin in 2.25 hours

N

Sensitive, reproducible, and convenient 96-well format requiring low sample volumes

N

Requires the Q-View™ Imaging System for analysis

Eotaxin Assay Performance Characteristics

Precision

Intra-assay (n=20)
Average Intra-assay CV: 7%

Sample Concentration (pg/ml) %CV
Sample 1

462.94

7%
Sample 2 29.68 7%
Sample 3 11.79 7%

 

Inter-assay (n=15)
Average Inter-assay CV: 8%

Sample Concentration (pg/ml) %CV
Sample 1 104.45 7%
Sample 2 896.61 5%
Sample 3 35.92 11%

 

Dilutional Linearity

Average percent linearity: 107%
Percent linearity range: 89 – 120%

Dilution Factor % Recovery
Serum HD Pooled Plasma Pooled Serum Stripped Serum
2 116%
4 109% 116% 115% 120%
8 89% 101% 100% 101%
Human eotaxin linearity

Q-Plex Multiplexed Products featuring Eotaxin

Q-Plex Products featuring Eotaxin

Human Multiplex Kits

Human Chemokine (9-plex)

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