Human FGF Analyte Overview

Human FGF

Also known as Basic, Human Basic Fibroblast Growth Factor, bFGF, FGF2, FGF-β

Basic Fibroblast Growth Factor (FGF-β, FGF-2) is a growth factor involved in the early development and maintenance of populations of embryonic and adult stem cells. During embryonic development it is critical for the pluripotency and self-renewal of embryonic stem cells, functioning to promote stem cell division and limit cellular differentiation. In adults is found in the basement membranes and extracellular matrix of blood vessels in many tissues, including neural, pituitary, adrenal cortex, corpus luteum, and placental tissues where it modulates wound healing, tissue repair, and neuronal function or degeneration. It has been demonstrated to be protective to heart tissue during a heart attack. Low levels of FGF-β are associated with anxiety disorders. FGF-β is upregulated in response to tumors and inflammation stimuli and is implicated in tumor growth.

Request a Quote

FGF Q-Plex™ details

Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 5,000 – 6.90 pg/ml
Lower Limit of Detection (LLD): 1.27 pg/ml
Volume Required Per Well: Min 25µl
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog No: 463549HU

N

Measure FGF in 2.25 hours

N

Sensitive, reproducible, and convenient 96-well format requiring low sample volumes

N

Requires the Q-View™ Imaging System for analysis

FGF Assay Performance Characteristics

Precision

Intra-assay (n=20)
Average Intra-assay CV: 9%

Sample	Concentration (pg/ml)	%CV
Sample 1	734.33	4%
Sample 2	56.47	9%
Sample 3	75.33	13%

Inter-assay (n=15)
Average Inter-assay CV: 11%

Sample	Concentration (pg/ml)	%CV
Sample 1	60.66	10%
Sample 2	693.19	9%
Sample 3	143.70	15%

Dilutional Linearity

Average percent linearity: 103%
Percent linearity range: 85 – 118%

Dilution Factor	% Recovery
Dilution Factor	Pooled Plasma	Pooled Serum	Serum HE
2	108%	113%	102%
4	118%	95%	85%
8			96%

Q-Plex Multiplexed Products featuring FGF

Q-Plex Products featuring FGF

Human Multiplex Kits

Human Angiogenesis (9-plex)

As researchers and problem solvers ourselves, we understand the value of sound data. We are proud to be a part of research that can better the world. We value the relationships, partnerships, and friendships that we have built with the people who trust and use our technology. We are committed to building these relationships. You can count on us to answer the phone and take time to thoroughly address questions or concerns about any of our products. In an industry that is reputed for grandiose claims, we trust that our quality standards and our customer service set us apart from the competition. If you’re happy with our products or if you think we can do something better, we hope you will let us know.



1-888-782-6797

