Human FGF Analyte Overview
Human FGF
Also known as Basic, Human Basic Fibroblast Growth Factor, bFGF, FGF2, FGF-β

FGF Q-Plex™ details
Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 5,000 – 6.90 pg/ml
Lower Limit of Detection (LLD): 1.27 pg/ml
Volume Required Per Well: Min 25µl
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog No: 463549HU
Measure FGF in 2.25 hours
Sensitive, reproducible, and convenient 96-well format requiring low sample volumes
Requires the Q-View™ Imaging System for analysis
FGF Assay Performance Characteristics
Precision
Intra-assay (n=20)
Average Intra-assay CV: 9%
Sample | Concentration (pg/ml) | %CV |
Sample 1 |
734.33 |
4% |
Sample 2 | 56.47 | 9% |
Sample 3 | 75.33 | 13% |
Inter-assay (n=15)
Average Inter-assay CV: 11%
Sample | Concentration (pg/ml) | %CV |
Sample 1 | 60.66 | 10% |
Sample 2 | 693.19 | 9% |
Sample 3 | 143.70 | 15% |
Dilutional Linearity
Average percent linearity: 103%
Percent linearity range: 85 – 118%
Dilution Factor | % Recovery | |||
Pooled Plasma | Pooled Serum | Serum HE | ||
2 | 108% | 113% | 102% | |
4 | 118% | 95% | 85% | |
8 | 96% |

Q-Plex Multiplexed Products featuring FGF
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