Human FGF Analyte Overview

Human FGF

Also known as Human Basic Fibroblast Growth Factor, bFGF, FGF2, FGF-β

Basic Fibroblast Growth Factor (FGF-β, FGF-2) is a growth factor involved in the early development and maintenance of populations of embryonic and adult stem cells. During embryonic development it is critical for the pluripotency and self-renewal of embryonic stem cells, functioning to promote stem cell division and limit cellular differentiation. In adults is found in the basement membranes and extracellular matrix of blood vessels in many tissues, including neural, pituitary, adrenal cortex, corpus luteum, and placental tissues where it modulates wound healing, tissue repair, and neuronal function or degeneration. It has been demonstrated to be protective to heart tissue during a heart attack. Low levels of FGF-β are associated with anxiety disorders. FGF-β is upregulated in response to tumors and inflammation stimuli and is implicated in tumor growth.
Human FGF Standard Curve
Request a Quote

Q-Plex™ Details

Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 5,000 – 6.90 pg/mL
Lower Limit of Detection (LLD): 1.27 pg/mL
Volume Required Per Well: Min. 25µL
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog #: 463549HU

Features & Requirements

  • Maesure FGF in 2.25 hours
  • Sensitive, reproducible, and convenient 96-well format requiring low sample volumes
  • Requires the Q-View™ Imaging System for analysis

Assay Performance Characteristics

Precision

Sample Concentration (pg/ml) %CV
Sample 1 734.33 4%
Sample 2 56.47 9%
Sample 3 75.33 13%

Intra-assay (n=20)
Average Intra-assay CV: 9%

Sample Concentration (pg/ml) %CV
Sample 1 60.66 10%
Sample 2 693.19 9%
Sample 3 143.70 15%

Inter-assay (n=15)
Average Inter-assay CV: 11%

Dilutional Linearity

Dilution Factor % Recovery
Pooled Plasma Pooled Serum Serum HE
2 108% 113% 102%
4 118% 95% 85%
8 96%

Average percent linearity: 103%
Percent linearity range: 85 – 118%

Human FGF Linearity

Featured Q-Plex Products 

Q-Plex™ Human Angiogenesis (9-Plex)

The Q-Plex Human Angiogenesis (9-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of Ang-2, FGF, HGF, IL-8, PDGF-BB, TIMP-1, TIMP-2, TNFα, and VEGF.

View Kit

Not Finding What You Need?

Customize a Kit

Not seeing the biomarker or analyte you need? Customize your kit! The Q-Plex™ Custom Assays are fully quantitative ELISA-based Chemiluminescent assay kits allowing the concurrent measurement of up to 18 biomarkers or analytes.

Customize a Kit

Send in Your Samples

Choose up to 18 different biomarkers that will work best for your research. Send in your samples and let us run them for you. 

Get Started

Contact a Representative

Have questions about the kit or interested in more information? Contact one of our representative to get all your questions answered. 

Contact Us

As researchers and problem solvers ourselves, we understand the value of sound data. We are proud to be a part of research that can better the world. We value the relationships, partnerships, and friendships that we have built with the people who trust and use our technology. We are committed to building these relationships. You can count on us to answer the phone and take time to thoroughly address questions or concerns about any of our products. In an industry that is reputed for grandiose claims, we trust that our quality standards and our customer service set us apart from the competition. If you’re happy with our products or if you think we can do something better, we hope you will let us know.