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Human IFNα Analyte Overview

Human IFNa

Also known as IFNα, IFN alpha, interferon gamma, type I interferon

Interferon alpha (IFN⍺) is a member of the interferon type I family of immune system proteins that bind to the INF⍺/β receptor complex. IFN⍺ plays a role in innate viral immunity and inflammatory responses. It is produced primarily by dendritic cells and leukocytes, however its production is inhibited by interleukin 10. There are multiple subtypes of IFN⍺ that each play unique roles in the immune system, including activating natural killer (NK) cells, stimulating B cell proliferation, promoting the production of human leukocyte antigen (HLA) types I and II, activating dendritic cells to promote an immune response. The interleukin has been found to limit the proliferation of cancer cells by halting the cell cycle and inducing apoptosis. IFN⍺ is commonly produced as a therapeutic under the name interferon alfa to treat a number of cancers and viral diseases, including leukemia, melanoma, and hepatitis B and C infections.

IFNα Q-Plex™ details

Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma, Heparin Plasma
Assay Range: 400 – 0.72 pg/ml
Lower Limit of Detection (LLD): 0.36 pg/ml
Volume Required Per Well: Min 25µl
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog No: 

N

Measure IFNα in 2.25 hours

N

Sensitive, reproducible, and convenient 96-well format requiring low sample volumes

N

Requires the Q-View™ Imaging System for analysis

IFNα Assay Performance Characteristics

Precision

Intra-assay (n=20)
Average Intra-assay CV: 5%

Sample Concentration (pg/ml) %CV
Sample 1

169

6%
Sample 2 123 4%
Sample 3 125 5%

 

Inter-assay (n=10)
Average Inter-assay CV: 9%

Sample Concentration (pg/ml) %CV
Sample 1 150.40 8%
Sample 2 114.50 10%
Sample 3 110.70 9%

 

Dilutional Linearity

Average percent linearity: 100%
Percent linearity range: 94 – 108%

Dilution Factor % Recovery
Serum 1 Serum 2 EDTA Plasma 1 EDTA Plasma 2
2 108% 94% 94% 101%
4 108% 104% 97% 95%
8 104% 98% 98% 96%
human IFNa linearity

Q-Plex Multiplexed Products featuring IFNα

Q-Plex Products featuring IFNα

Human Multiplex Kits

 Human Cytokine Release (16-plex)

 

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