Human MCP-2 Analyte Overview

Human MCP-2

Also known as Monocyte Chemotactic Protein-2, Chemokine Ligand 8, CCL8

Monocyte chemoattractant protein 2 (MCP-2), also known as Chemokine (C-C motif) ligand 8 (CCL8), is a chemokine involved in early stages of an immune responses. It interacts with cellular receptors CCR1, CCR2B, and CCR5 to attract and activate many types of immune cells, including monocytes, T-cells, and natural killer cells during and immune response and mast cells, eosinophils, and basophils in the case of an allergic response. MCP-2 is often expressed by stromal tissues that have been exposed to pathogens. It recruits immature monocytes to sites of inflammation to mature. Since MCP-2 demonstrates high affinity binding to receptor CCR5, a common HIV co-receptor, it has the potential to be HIV protective. It is often found in high levels in patients experiencing metastasis of a tumor and may play a role in promoting metastasis.

MCP-2 Q-Plex™ details

Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 940 – 1.29 pg/ml
Lower Limit of Detection (LLD): 1.27 pg/ml
Volume Required Per Well: Min 25µl
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog No: 467149HU

N

Measure MCP-2 in 2.25 hours

N

Sensitive, reproducible, and convenient 96-well format requiring low sample volumes

N

Requires the Q-View™ Imaging System for analysis

MCP-2 Assay Performance Characteristics

Precision

Intra-assay (n=20)
Average Intra-assay CV: 6%

Sample Concentration (pg/ml) %CV
Sample 1

140.42

7%
Sample 2 14.23 6%
Sample 3 5.19 5%

 

Inter-assay (n=15)
Average Inter-assay CV: 11%

Sample Concentration (pg/ml) %CV
Sample 1 116.59 11%
Sample 2 60.53 13%
Sample 3 111.93 9%

 

Dilutional Linearity

Average percent linearity: 103%
Percent linearity range: 79 – 122%

Dilution Factor % Recovery
Cell Culture Supernatant Activated Plasma Pooled Serum Serum HF
2 98% 111% 110% 101%
4 105% 89% 111% 107%
8 101% 79% 98% 122%
human MCP-2 linearity

Q-Plex Multiplexed Products featuring MCP-2

Q-Plex Products featuring MCP-2

Human Multiplex Kits

Human Chemokine (9-plex)

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