Q-Plex™ Human Pneumococcal IgG (14-Plex)

Q-Plex Human Pneumococcal IgG (14-Plex)

List of Serotypes: 1, 3, 4, 5, 6B, 7F, 8, 9N, 9V, 12F, 14, 18C, 19F,  23F 

  • Species Specificity: Human
  • Validated Sample Type: Serum
  • Sample Volume Required: 0.25 µL
  • Estimated Assay Length: Less than 1.25 hours
  • Intra-Assay Cross Reactivity: No significant interference observed. <1% cross-reactivity observed. 
  • Assay Type: Indirect
  • Detection Method: Chemiluminescent
  • Multiplex Format: 96-well solid plate
  • Catalog 14-Plex: #481849HU

Q-Plex™ Human Pneumococcal IgG (14-Plex)

Legend: Pneumovax 23 = PNX, Prevnar = P7, Prevnar 13 = P13, Prevnar 20 = P20, Vaxneuvance = V15

Serotype Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection Precision* (Inter-assay) Precision* (Intra-assay) Average Linearity Vaccines
1 Indirect 0.85 - 0.00117 (μg/mL) 0.259 (μg/mL) 0.000468 (μg/mL) 0.000583 (μg/mL) 6% 7% 97% PNX, P13, P20, V15
3 Indirect 0.0145 - 0.00020 (μg/mL) 0.294 (μg/mL) 0.000533 (μg/mL) 9.95E-05 (μg/mL) 12% 14% 99% PNX, P13, P20, V15
4 Indirect 0.33 - 0.00045 (μg/mL) 0.154 (μg/mL) 0.000273 (μg/mL) 0.000226 (μg/mL) 5% 6% 99% PNX, P7, P13, P20, V15
5 Indirect 0.75 - 0.00103 (μg/mL) 0.371 (μg/mL) 0.000676 (μg/mL) 0.000514 (μg/mL) 8% 8% 102% PNX, P13, P20, V15
6B Indirect 0.91 - 0.00125 (μg/mL) 0.133 (μg/mL) 0.000247 (μg/mL) 0.000624 (μg/mL) 8% 9% 103% PNX, P7, P13, P20, V15
7F Indirect 0.83 - 0.00114 (μg/mL) 0.392 (μg/mL) 0.00286 (μg/mL) 0.000569 (μg/mL) 6% 7% 100% PNX, P13, P20, V15
8 Indirect 1.42 - 0.00195 (μg/mL) 0.133 (μg/mL) 0.000962 (μg/mL) 0.000974 (μg/mL) 7% 8% 97% PNX, P20
9N Indirect 0.87 - 0.00119 (μg/mL) 0.154 (μg/mL) 0.001105 (μg/mL) 0.000597 (μg/mL) 8% 10% 102% PNX
9V Indirect 0.64 - 0.00088 (μg/mL) 0.203 (μg/mL) 0.00143 (μg/mL) 0.000439 (μg/mL) 8% 8% 102% PNX, P7, P13, P20, V15
12F Indirect 0.22 - 0.00030 (μg/mL) 0.07 (μg/mL) 0.00052 (μg/mL) 0.000151 (μg/mL) 11% 9% 93% PNX, P20
14 Indirect 3.8 - 0.00521 (μg/mL) 0.119 (μg/mL) 0.000871 (μg/mL) 0.002606 (μg/mL) 7% 7% 98% PNX, P7, P13, P20, V15
18C Indirect 0.73 - 0.00100 (μg/mL) 0.217 (μg/mL) 0.00156 (μg/mL) 0.000501 (μg/mL) 5% 5% 100% PNX, P7, P13, P20, V15
19F Indirect 1.46 - 0.00200 (μg/mL) 0.924 (μg/mL) 0.00676 (μg/mL) 0.001001 (μg/mL) 9% 12% 100% PNX, P7, P13, P20, V15
23F Indirect 0.6 - 0.00082 (μg/mL) 0.084 (μg/mL) 0.000624 (μg/mL) 0.000412 (μg/mL) 10% 10% 99% PNX, P7, P13, P20, V15
*Intra-assay and inter-assay precision are calculated with n=20 and n=10 replicates, respectively.

Frequently Bought Together

The Q-Plex™ Human Pneumococcal IgG (14-Plex) plate detects antibodies recognizing 14 common serotypes individuals are vaccinated against while the second kit, the Q-Plex™ Human Pneumococcal IgG (9-Plex), detects antibodies recognizing 9 additional serotypes commonly used as vaccination targets. Limits for these assays were set using the 007sp Human Anti-Pneumococcal capsule Reference Serum.

Pneumococcal 14-Plex kit image

Q-Plex Human Pneumococcal IgG (9-Plex)

Serotypes: 2, 10A, 11A, 15B, 17F, 19A, 20, 22F, 33F


The bacterium Streptococcus pneumoniae can cause potentially deadly infections in children and the elderly, including pneumonia and meningitis. Risk of infection and disease severity varies with age of the patient and a host of other factors including geographical location, ethnic background, and time of year. More than 90 serotypes are known, though less than 25 are responsible for most cases, with virulence often differing between serotypes.

Principle of the Assay

The Q-Plex™ Human Pneumococcal IgG (14-Plex) is a chemiluminescent multiplex ELISA run on a 96-well plate. This pneumococcal serotype test quantitatively measures antibody levels corresponding to the serotypes found in pure polysaccharide vaccines like the Pneumovax®23 vaccine as well as protein conjugated vaccines such as Prevnar 7, Prevnar 13, Prevnar 20, and Vaxneuvance.

Antibodies play an essential role in the protection against pneumococcal disease. In humans, serum antibodies typically act against the polysaccharide capsule in a serotype specific manner. There are multiple vaccination options for protection against S. pneumoniae, with the major difference being the number and composition of specific serotypes present in the vaccine.

Kit Content

Each kit contains a 96-well plate, featuring the relevant biomarker panel in each well, and all reagents required to perform testing.

Reagents Include:

  • Detection Mix
  • Calibrators
  • Substrates
  • Sample Diluents
  • Streptavidin HRP
  • Wash Buffer


All Quansys Q-Plex™ Multiplex and Singleplex assays require the use of the Q-View™ Software to read and interpret the test results. A fully-functional, free trial version of the software is available to download, install, and use to analyze your first kit(s). At the end of the trial period, a purchased license is required to continue the use of the Q-View software.

Q-Plex™ Arrays are developed and optimized to work with Q-View™ Imagers. Quansys does not guarantee the results obtained from other imagers and not all imagers are compatible with Quansys Q-Plex Arrays.

Other Equipment Recommendations

Publication Library

Search our publication library for all the peer-reviewed journals and research products that have utilized Q-Plex™ technology here.

In-Kit Certificate of Analysis

Search and download Certificates of Analysis for your Quansys Biosciences kits here.

Not Finding What You Need?

Customize a Kit

Not seeing the biomarker or analyte you need? Customize your kit! The Q-Plex™ Custom Assays are fully quantitative ELISA-based Chemiluminescent assay kits allowing the concurrent measurement of up to 18 biomarkers or analytes.

Send in Your Samples

Choose up to 18 different biomarkers that will work best for your research. Send in your samples and let us run them for you. 

Contact a Representative

Have questions about the kit or interested in more information? Contact one of our representative to get all your questions answered. 

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