Q-Plex ™ Mouse Cytokine Panel 3 HS (4-Plex)

Q-Plex Mouse Cytokine Panel 3 HS (4-Plex)

For measurement of IL-2, IL-4, IL-6, TNFα

  • Species Specificity: Mouse
  • Validated Sample Type: Serum, Plasma
  • Sample Volume Required: Min. 25 µL
  • Estimated Assay Length: 5.5 hours
  • Intra-Assay Cross-Reactivity: All analytes <1% intra-panel cross-reactivity
  • Assay Type: Planar based multiplexed ELISA
  • Detection Method: Chemiluminescent
  • Multiplex Format: 96-well solid plate
  • Catalog #: 111249MS
Analyte Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection Precision (Inter-assay) Precision (Intra-assay) Average Linearity
IL-2 Sandwich 360 - 0.49 (pg/mL) 161 (pg/mL) 0.41 (pg/mL) 0.25 (pg/mL) 5.1% 6.6% 88%
IL-4 Sandwich 400 - 0.55 (pg/mL) 126 (pg/mL) 0.32 (pg/mL) 0.27 (pg/mL) 6.2% 6.6% 98%
IL-6 Sandwich 504 - 0.69 (pg/mL) 819 (pg/mL) 2.09 (pg/mL) 0.35 (pg/mL) 7.3% 6.1% 106%
TNFα Sandwich 429 - 0.59 (pg/mL) 217 (pg/mL) 0.55 (pg/mL) 0.29 (pg/mL) 6.9% 5.2% 104%

Product Overview

The Q-Plex Mouse Cytokine Panel 3 HS (4-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing concurrent measurement of four biomarkers or analytes (IL-2, IL-4, IL-6, TNFα).

Each kit contains a 96-well plate, with each well featuring the relevant biomarker panel, and all the reagents required to perform testing. Q-Plex™ plates are built by absorbing four distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.

Using just 25 µL of sample per well, up to 80 samples can be assayed for all four markers in the panel in 5.5 hours. Q-Plex™ Arrays provide scientists with an easy-to-use and cost-effective means of generating a cytokine profile for each sample.

Kit Content

Each kit contains a 96-well plate, featuring the relevant biomarker panel in each well, and all reagents required to perform testing.

Reagents Include:

  • Calibrator
  • Detection Mix
  • Substrate
  • Sample Diluents
  • Streptavidin HRP
  • Wash Buffer


All Quansys Q-Plex™ Multiplex and Singleplex assays require the use of the Q-View™ software to read and interpret the test results. A fully-functional, free trial version of the software is available to download, install, and use to analyze your first kit(s). At the end of the trial period, a purchased license is required to continue the use of the Q-View software.

Q-Plex arrays are developed and optimized to work with Q-View imagers. Quansys does not guarantee the results obtained from other imagers and not all imagers are compatible with Quansys Q-Plex arrays.

Other Equipment Recommendations

    Publication Library

    Search our publication library for all the peer-reviewed journals and research products that have utilized Q-Plex™ technology here.

    In-Kit Certificate of Analysis

    Search and download Certificates of Analysis for your Quansys Biosciences kits here.

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    Customize a Kit

    Not seeing the biomarker or analyte you need? Customize your kit! The Q-Plex™ Custom Assays are fully quantitative ELISA-based Chemiluminescent assay kits allowing the concurrent measurement of up to 18 biomarkers or analytes.

    Send in Your Samples

    Choose up to 18 different biomarkers that will work best for your research. Send in your samples and let us run them for you. 

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    Have questions about the kit or interested in more information? Contact one of our representative to get all your questions answered. 

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