Human IL-12p70 Analyte Overview

Human IL-12p70

Also known as CLMF p35, interleukin 12, p35, interleukin 12A, NKSF1, Active Heterodimer of Interleukin-12

Interleukin 12 p70 (IL-12p70) is a subunit of interleukin 12 (IL-12) a cytokine that has a broad range of biological activities in the immune system. The complete IL-12 unit aids in the differentiation of naïve T cells into Th1 cells and regulates the activity of natural killer cells. It also stimulates the production of T cells, IFN-γ, and TNF-α. IL-12 inhibits the formation of new blood vessels and may play a role in autoimmune disorders.

Q-Plex™ Details

Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 3,000 – 5.3 pg/mL
Lower Limit of Detection (LLD): 2.65 pg/mL
Volume Required Per Well: Min. 25µL
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog #: 460449HU

Features & Requirements

  • Measure IL-12p70 in 2.25 hours
  • Sensitive, reproducible, and convenient 96-well format requiring low sample volumes
  • Requires the Q-View™ Imaging System for analysis

Assay Performance Characteristics


Intra-assay (n=20)
Average Intra-assay CV: 7%

Sample Concentration (pg/ml) %CV
Sample 1


Sample 2 50.53 5%
Sample 3 15.66 8%


Inter-assay (n=15)
Average Inter-assay CV: 7%

Sample Concentration (pg/ml) %CV
Sample 1 55.64 9%
Sample 2 410.85 7%
Sample 3 704.21 6%


Dilutional Linearity

Average percent linearity: 103%
Percent linearity range: 98 – 110%

Dilution Factor % Recovery
Serum HH Pooled Plasma Pooled Serum Serum HF
2 105% 107% 98% 108%
4 100% 102% 100% 103%
8 108% 100% 99% 110%
Human il-12p70 linearity

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