Human Cytokine - Stripwells (16-Plex)

Q-Plex™ Product Overview

Product Overview

The Q-Plex™ Human Cytokine – Stripwells (16-plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 16 biomarkers or analytes. Using only 25 µl of sample per well, up to 80 samples can be assayed for all 16 markers in the panel within 2.25 hours. The Q-Plex™ Human Cytokine – Stripwells (16-Plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Q-Plex plates are built by adsorbing 16 distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.

Q-Plex™ Human Cytokine – Stripwells (16-plex)

For measurement of IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-15, IL-17, IL-23, IFNγ, TNFα, TNFβ

Species: Human
Sample Type: Serum, EDTA/Heparin Plasma
Volume Required per Well: Min 25 µl
Assay Length: 2.25 hours
Specificity: All analytes < 1% intra-panel cross-reactivity
Assay Type: Planar Based Multiplexed ELISA
Detection Method: Chemiluminescent
Multiplex Format: 96-well solid plate
Catalog: #110333HU
Assay Ranges
Units Calibrator Range LLD
IL-1α pg/ml 4000 – 5.49 5.43
IL-1β pg/ml 10000 - 13.72 10.14
IL-2 pg/ml 4000 – 5.49 3.16
IL-4 pg/ml 2000 – 2.74 2.14
IL-5 pg/ml 3000 – 4.12 2.90
IL-6 pg/ml 3000 – 4.11 3.47
IL-8 pg/ml 2000 – 2.74 2.70
IL-10 pg/ml 3000 – 4.11 4.10
IL-12p70 pg/ml 3000 – 4.12 3.20
IL-13 pg/ml 3000 – 4.12 2.86
IL-15 pg/ml 3000 – 4.12 1.92
IL-17 pg/ml 4000 - 5.49 5.40
IL-23 pg/ml 30000 - 41.15 25.00
IFNγ pg/ml 8000 - 10.97 4.95
TNFα pg/ml 4000 - 5.49 5.20
TNFβ pg/ml 4000 - 5.49 5.29
Interleuken 1 alpha (IL-1α), also known as hematopoietin 1, is a cytokine that plays a role in inflammation, fever, and sepsis. It is produced by macrophages, neutrophils, and endothelial and epithelial cells. In the skin, IL-1α is regularly produced at high levels and helps maintain skin barrier function against pathogens as well as overall skin condition. As part of the immune response, its production is by microbial exposure. It is responsible for releasing inflammatory mediators and acute phase proteins. The severity of inflammation, fever, and sepsis are associated with circulating IL-1α levels. IL-1α inhibitors are a potential target for therapeutics to treat and prevent these conditions as well as mediate skin conditions such as acne. Additionally, IL-1a is part of the pathway that activates tumor necrosis factor-alpha and helps kill some kinds of cancer cells. It also supports stem cell expansion and osteoclast formation following bone marrow transplant.
Interleukin 1 beta (IL-1β) is a cytokine involved in pro-inflammatory responses. It is produced by activated macrophages, natural killer cells, monocytes, and neutrophils in response to wounds, infections, or microbial endotoxins and is processed to its active state by caspase 1. It mediates inflammatory responses and plays a role in cell proliferation, apoptosis, and differentiation. IL-1β inflammation within the central nervous system is known to result in pain and hypersensitivity. High levels of IL-1β are associated with autoinflammatory diseases, particularly the group of rare heterozygous dominant diseases known as cryopyrin-associated periodic syndromes, that often affect the skin, joints, eyes, and nervous system. Inhibition of IL-1β treats these diseases and eases symptoms in common conditions such as type 2 diabetes, cardiac failure, gout, and rheumatoid arthritis. Dysregulated IL-1β is associated with tumor formation in a wide variety of cancers.
Interleukin-2 (IL-2) cytokine signaling molecule in the immune system that mediates the activity of white blood cells, particularly lymphocytes, during an immune response. IL-2 promotes the differentiation of T cells into memory T cells after exposure to a novel pathogen, making it a critical component of the active immune system and long-term immunity to disease. In the thymus, IL-2 supports the formation of regulatory T cells, which work to prevent autoimmune diseases by eliminating overactive immune cells. Since IL-2 helps increase immune cell production and regulate large scale activities of the immune system, it is commonly used as a cancer treatment for patients with certain kinds of cancers, including kidney and skin cancers.
Interleukin 4 (IL-4) is a cytokine that has pleiotropic effects during active immune responses. Its primary role is to induce differentiation of helper T-cells and active B cells. IL-4’s other roles include regulating immunity, upregulating MHC class II production, supporting the proliferation of epithelial cells, and downregulating the production of Th1 cells, macrophages, and some lines of dendritic cells. IL-4 activates two different receptor complexes, one expressed on hematopoietic cells and another found on nonhematopoietic cells. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, eosinophils, and basophils. Its responsibilities include cell proliferation, immunoglobulin class switch (IgG4, IgE of human B cells), survival, priming and chemotaxis of mast cells, eosinophils, and basophils. Dysregulation of IL-4 is implicated in many autoimmune disorders, particularly the development of allergies. Overexpression of the cytokine is associated with tumor growth in many kinds of cancer. People infected with HIV often produce higher levels of IL-4. Human, mouse, and rat IL-4 are species-specific in their activities.
Interleukin 5 (IL-5) is a cytokine that modules immune system activities. It is secreted by activated TH2 cells, mast cells, eosinophils, EBV-transformed B cells, Reed-Sternberg cells (found in Hodgkin’s Disease) and iNKT cells. It increases immunoglobulin secretion, stimulates B-cell growth, and promotes eosinophilic and basophilic white blood cell differentiation and activation, as well as increasing mobilization of eosinophils from bone marrow. In a healthy person, IL-5 supports immune responses to multicellular parasites through eosinophil proliferation. IL-5 is implicated in diseases that are characterized by high levels of eosinophils, particularly severe allergic diseases, such as hay fever and asthma.
Interleuken 6 (IL-6) has the capability to behave as both a pro-inflammatory cytokine and an anti-inflammation myokine. As a cytokine, it is secreted by macrophages in response to microbial activity, to regulate the progression of fever through raising the temperature of muscle and fat, as well as crossing the blood-brain barrier to alter the body’s temperature set point in the hypothalamus. It acts through T cells and macrophages fighting infection and causing inflammation. As a myokine, it is released by contracting muscles during exercise, and inhibits the activity of pro-inflammatory agent TNF-alpha, reducing system-wide inflammation. In addition to its functions in the immune system, IL-6 aids in bone remodeling and maintenance by stimulating the production of osteoclasts. Clinically, IL-6 is implicated in the inflammatory and autoimmune symptoms of many diseases, including diabetes, asthma, atherosclerosis, depression, Alzheimer’s disease, rheumatoid arthritis, and many cancers. High levels of IL-6 are common in advanced cancer patients and are correlated with low survival rates. IL-6 has the ability to induce epigenetic modifications of gene transcription in the brain, and these IL-6 modifications are associated with schizophrenia and major depressive disorder.
Interleukin 8 (IL-8), also known as CXCL8, is a chemokine that plays a role in the recruitment and regulation of white blood cells during an innate immune response. It is a strong chemoattractant for neutrophils and other granulocytes, bringing them to the site of infection and then stimulating them to engage in phagocytosis. It is commonly secreted by macrophages that have identified a foreign pathogen but may be emitted by any cell with toll-like receptors. IL-8 plays a key role in metastasis; it was first identified as a leukocyte chemoattractant that progresses melanoma by directly inducing angiogenesis and migration of melanoma cells. Serum IL-8 levels are directly correlated with the degree of metastatic melanoma and overall tumor count. High levels of IL-8 in a pregnant mother are associated with schizophrenia in her child and high levels in adults with schizophrenia reduces the effectiveness of antipsychotic medications. IL-8 dysregulation is also involved in cystic fibrosis, where it recruits an excessive number of white blood cells to the lungs, resulting in tissue damage.
Interleukin 10 (IL-10) is a class-2 anti-inflammatory cytokine, also known as human cytokine synthesis inhibitory factor (CSIF). It impacts many areas of immunoregulation and inflammation, with numerous pleiotropic effects in the immune system. As an anti-inflammatory, it downregulates MHC class II expression, inhibits the synthesis and activity of many inflammatory cytokines, and reduces co-stimulatory factors on macrophages. It also enhances the release of other anti-inflammatory mediators by monocytes and macrophage, particularly during exercise. It enhances B cell proliferation, differentiation, survival, and antibody production. IL-10 has anti-tumor effects, reducing tumor growth and decreasing metastatic burden in the body. IL-10 deficiency is associated with chronic inflammatory and autoimmune diseases, including multiple sclerosis, Crohn’s disease, inflammatory bowel disease, psoriasis, and rheumatoid arthritis. IL-10 is currently under investigation as a potential therapeutic for many conditions, including cancer treatments and reducing symptoms in inflammatory disorders.
Interleukin 12 p70 (IL-12p70) is a subunit of interleukin 12 (IL-12) a cytokine that has a broad range of biological activities in the immune system. The complete IL-12 unit aids in the differentiation of naïve T cells into Th1 cells and regulates the activity of natural killer cells. It also stimulates the production of T cells, IFN-γ, and TNF-α. IL-12 inhibits the formation of new blood vessels and may play a role in autoimmune disorders.
Interleukin-13 is an immunoregulatory cytokine that is involved numerous immune processes and a mediator of allergic inflammation responses. It is secreted primarily by activated Th2 cells, but also by smooth muscle cells, eosinophils, basophils, NK cells, mast cells, and helper T cells in response to pathogenic detection, particularly parasitic infections. IL-13 is commonly associated with airway activity during an immune response, since it regulates mucus secretion and airway responsiveness in the respiratory tract. It also encourages the production of IgE, which confers immunity to many helminth parasites, and IL-13 induces changes in the lungs and gut that encourage the expulsion of these parasites. Its effects on the maturation and differentiation of B cells are similar to that of IL-4, which it shares similar structure and activation pathways, however IL-13 appears to be less critical to B cell function than IL-4. IL-13 also suppresses the production of pro-inflammatory cytokines by macrophages. Due to its role in airway responses, dysregulation of IL-13 is associated with asthma and severe allergic responses.
Interleukin 15 (IL-15) is a common cytokine that is similar to IL-2 structurally and functionally and plays an important role in the innate immune system. IL-15 stimulates the proliferation of T-lymphocytes and natural killer cells, and assists in their maintenance, activation, and differentiation. Research on Interleukin 15 and its receptor complex indicate involvement in processes such as aging, cell maturation, and regulation of cellular processes. Overexpression of IL-15 is associated with pediatric asthma, Celiac disease, and non-alcoholic fatty liver disease, and anti-IL-15 therapeutics are under investigation for the treatment of these conditions. In cancer biology, IL-15 displays anti-tumor properties and is being reviewed as a component of both anti-viral and anti-tumor vaccines. IL-15 is a target of particular interest for patients with both HIV infection and cancer, as it appears to support improved both tumor reduction and improved immune function.
Interleukin 17 (IL-17) are a small family of similarly structured cytokines that plays a role in pro-inflammatory responses of the immune system, particularly allergic reactions. Each variant of IL-17 appears to have slightly nuanced function in different organs, but on the whole the cytokines behave similarly. IL-17 induces inflammation by stimulating the secretion of pro-inflammatory molecules such as IL-6, IL-8, GM-CSF, IL-1, TNF-α, and MCP-1 from their respective cell types. For this reason, IL-17 is linked to inflammatory and autoimmune diseases such as rheumatoid arthritis, asthma, lupus, psoriasis, and multiple scelrosis. Overactivity of IL-17 is commonly found in severe cases of asthma and psoriasis, where it contributes to inflammation that damages the airway and skin, respectively. IL-17 suppression is under investigation as a clinical target for reducing symptoms of inflammatory diseases, improving recovery following stroke, and treatment of some types of cancer.
Interferon gamma (IFNγ), also known as Type II interferon, is a cytokine that plays a critical role in the innate and adaptive immune systems. It serves as protection against viral infections, as well as some bacterial and protozoal infections, and plays a role in tumor control. It is capable of inhibiting viral replication and works to modulate immunological responses to multiple kinds of pathogens, with effects including promoting natural killer cell activity, activating macrophages to destroy foreign cells, increasing antigen presentation, and inducing the expression of numerous immune defense factors. IFNγ is primarily produced by natural killer and natural killer T cells during the innate immune response and by CD4 and CD8 cytotoxic T lymphocyte effector T cells as part of an adaptive response. It has been shown to halt growth and induce apoptosis in some types of tumors. During pregnancy, IFNγ aids in the remodeling of the uterine wall and placenta to accommodate a zygote.
Interleukin-23 (IL-23) is a heterodimeric cytokine consisting of two subunits, one called p40, which is shared with another cytokine, IL-12, and another called p19 (the IL-23 alpha subunit). IL-23 is an important part of the inflammatory response against infection. It promotes upregulation of the matrix metalloprotease MMP9, increases angiogenesis and reduces CD8+ T-cell infiltration. Recently, IL-23 has been implicated in the development of cancerous tumors. In conjunction with IL-6 and TGF-β1, IL-23 stimulates naive CD4+ T cells to differentiate into a novel subset of cells called Th17 cells, which are distinct from the classical Th1 and Th2 cells. Th17 cells produce IL-17, a proinflammatory cytokine that enhances T cell priming and stimulates the production of proinflammatory molecules such as IL-1, IL-6, TNF-alpha, NOS-2, and chemokines resulting in inflammation. Knockout mice deficient in either p40 or p19, or in either subunit of the IL-23 receptor (IL-23R and IL12R-β1) develop less severe symptoms of multiple sclerosis and inflammatory bowel disease highlighting the importance of IL-23 in the inflammatory pathway.
Interferon gamma (IFNγ), also known as Type II interferon, is a cytokine that plays a critical role in the innate and adaptive immune systems. It serves as protection against viral infections, as well as some bacterial and protozoal infections, and plays a role in tumor control. It is capable of inhibiting viral replication and works to modulate immunological responses to multiple kinds of pathogens, with effects including promoting natural killer cell activity, activating macrophages to destroy foreign cells, increasing antigen presentation, and inducing the expression of numerous immune defense factors. IFNγ is primarily produced by natural killer and natural killer T cells during the innate immune response and by CD4 and CD8 cytotoxic T lymphocyte effector T cells as part of an adaptive response. It has been shown to halt growth and induce apoptosis in some types of tumors. During pregnancy, IFNγ aids in the remodeling of the uterine wall and placenta to accommodate a zygote.
Tumor necrosis factor-beta (TNFβ), also known as lymphotoxin-alpha (LT-α), is a cytotoxic protein of the TNF superfamily, primarily expressed by T and B lymphocytes. As a multifunctional protein, TNFβ mediates a wide range of activities in the innate immune system. TNFβ displays strong anti-prolific abilities and destroys tumor cell lines, making it an important regulator of cancers. It is also involved in regulating cell survival, proliferation, differentiation, and apoptosis. Dysregulation of TNFβ can also lead to tumor promotion when its overproduction leads to a prolonged inflammatory response. TNFβ is critical for normal growth of the gastrointestinal system during embryonic development and maintains an important role in the gut in adults by regulating the immune activity in the intestines. During early development, TNFβ also mediates the formation of the lymph nodes. TNFβ is implicated in the progression of many autoimmune diseases.

 

Each kit contains a 96-well plate, featuring the relevant biomarker panel in each well, and all reagents required to perform testing.

Reagents Include:

  • Calibrator
  • Detection Mix
  • Substrate
  • Sample Diluents
  • Streptavidin HRP
  • Wash Buffer

All Quansys Q-Plex™ Multiplex and Singleplex assays require the use of the Q-View™ software to read and interpret the test results. A fully-functional, free trial version of the software is available to download, install, and use to analyze your first kit(s). At the end of the trial period, a purchased license is required to continue the use of the Q-View software.

Q-Plex arrays are developed and optimized to work with Q-View imagers. Quansys does not guarantee the results obtained from other imagers and not all imagers are compatible with Quansys Q-Plex arrays.

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