Human IL-1α Analyte Overview

Human IL-1α

Also known as Interleuken 1 alpha, fibroblast-activating factor (FAF), B-cell-activating factor (BAF), leukocyte endogenous mediator (LEM), epidermal cell-derived thymocyte-activating factor (ETAF), serum amyloid A inducer of hepatocyte-stimulating factor (HS), catabolin, hemopoetin-1 (H-1), endogenous pyrogen (EP), osteoclast-activating factor (OAF), proteolysis-inducing factor (PIF)

Interleuken 1 alpha (IL-1a or IL-1α), also known as hematopoietin 1, is a cytokine that plays a role in inflammation, fever, and sepsis. It is produced by macrophages, neutrophils, and endothelial and epithelial cells. In the skin, IL-1a is regularly produced at high levels and helps maintain skin barrier function against pathogens as well as overall skin condition. As part of the immune response, its production is by microbial exposure. It is responsible for releasing inflammatory mediators and acute phase proteins. The severity of inflammation, fever, and sepsis are associated with circulating IL-1a levels. IL-1a inhibitors are a potential target for therapeutics to treat and prevent these conditions as well as mediate skin conditions such as acne. Additionally, IL-1a is part of the pathway that activates tumor necrosis factor-alpha and helps kill some kinds of cancer cells. It also supports stem cell expansion and osteoclast formation following bone marrow transplant.

Q-Plex™ Details

Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 4,000 – 5.49 pg/mL
Lower Limit of Detection (LLD): 5.43 ng/mL
Volume Required Per Well: Min. 25µL
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog #: 465449HU

Features & Requirements

  • Measures IL-1α
    in 2.25 hours
  • Sensitive, reproducible, and convenient 96-well format requiring low sample volumes
  • Requires the Q-View™ Imaging System for analysis

Assay Performance Characteristics


Intra-assay (n=20)
Average Intra-assay CV: 3%

Sample Concentration (pg/ml) %CV
Sample 1 608.31 2%
Sample 2 48.38 2%
Sample 3 16.97 4%


Inter-assay (n=15)
Average Inter-assay CV: 6%

Sample Concentration (pg/ml) %CV
Sample 1 826.63 7%
Sample 2 646.36 3%
Sample 3 51.78 6%


Dilutional Linearity

Average percent linearity: 86%
Percent linearity range: 81 – 91%

Dilution Factor % Recovery
Serum HA Pooled Serum Pooled Plasma Plasma HD
2 81% 90% 87% 82%
4 89% 91% 86% 83%
8 89% 86% 83% 86%
IL-1a Linearity

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