Q-Plex™ Human Cytokine Screen (16-Plex)

Q-Plex Human Cytokine Screen (16-Plex)

For measurement of IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-15, IL-17A, IL-23, TNFα, TNFβ

  • Species Specificity: Human

  • Validated Sample Type: Serum, EDTA/Heparin Plasma

  • Sample Volume Required: Min. 25 µl

  • Estimated Assay Length: 2.25 hours

  • Intra-Assay Cross-Reactivity: All analytes < 1% intra-panel cross-reactivity

  • Assay Type: Planar Based Multiplexed ELISA

  • Detection Method: Chemiluminescent

  • Multiplex Format: 96-well solid plate

  • Catalog #: 110933HU

Analyte Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection Precision* (Inter-assay) Precision* (Intra-assay) Average* Linearity
IFNγ Sandwich  8000 -10.97 (pg/mL) 5600 (pg/mL) 14.3 (pg/mL) 4.95 (pg/mL) 8% 4% 97%
IL-1α Sandwich  4000 -5.49 (pg/mL)  2800 (pg/mL) 7.1 (pg/mL) 5.43 (pg/mL) 6% 7% 86%
IL-1β Sandwich  12226 -16.77 (pg/mL) 8558 (pg/mL) 21.8 (pg/mL) 12.40 (pg/mL) 8% 5% 94%
IL-2 Sandwich 5213 – 7.15 (pg/mL) 3649 (pg/mL) 9.3 (pg/mL) 4.12 (pg/mL) 6% 4% 99%
IL-4 Sandwich 1676 – 2.30 (pg/mL) 1173 (pg/mL) 3.0 (pg/mL) 1.79 (pg/mL) 6% 6% 94%
IL-5 Sandwich 3000 – 4.12 (pg/mL) 2100 (pg/mL) 5.3 (pg/mL) 2.90 (pg/mL) 10% 7% 95%
IL-6 Sandwich 3000 – 4.12 (pg/mL) 2100 (pg/mL) 5.3 (pg/mL)  3.47 (pg/mL) 7% 4% 104%
IL-8 Sandwich 2000 – 2.74 (pg/mL) 1400 (pg/mL) 3.6 (pg/mL) 1.37 (pg/mL) 12% 7% 100%
IL-10 Sandwich 8039 – 11.03 (pg/mL) 5627 (pg/mL) 14.3 (pg/mL) 10.99 (pg/mL) 7% 5% 103%
IL-12p70 Sandwich 3000 – 4.12 (pg/mL) 2100 (pg/mL) 5.3 (pg/mL)  3.20 (pg/mL) 7% 7% 103%
IL-13 Sandwich 3000 – 4.12 (pg/mL) 2100 (pg/mL) 5.3 (pg/mL) 4.12 (pg/mL) 6% 7% 98%
IL-15 Sandwich  10733 – 14.72 (pg/mL) 7513 (pg/mL) 19.1 (pg/mL) 6.87 (pg/mL) 10% 7% 96%
IL-17A Sandwich 16772 – 23.01 (pg/mL) 11740 (pg/mL) 29.9 (pg/mL) 22.57 (pg/mL) 7% 7% 95%
IL-23 Sandwich 41700 – 57.20 (pg/mL) 21000 (pg/mL) 120 (pg/mL) 48.65 (pg/mL) 10% 5% 105%
TNFα Sandwich  6500 – 8.92 (pg/mL) 4522 (pg/mL) 11.5 (pg/mL) 7.74 (pg/mL) 6% 5% 98%
TNFβ Sandwich 4000 – 5.49 (pg/mL) 2800 (pg/mL) 7.1 (pg/mL) 5.29 (pg/mL) 8% 5% 96%

Product Overview

The Q-Plex Human Cytokine Screen (16-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 16 biomarkers or analytes. Multiple analyte measurements are achieved by adsorbing 16 distinct capture antibodies in a defined array to the bottom of each well of a 96-well plate. Our high-quality reagents help ensure the accuracy and precision of your results.

Using only 25 µL of sample per well, up to 80 samples can be assayed for all 16 markers in the panel within 2.25 hours. The Q-Plex Human Cytokine Screen (16-Plex) provides researchers with an easy-to-use and cost-effective means of generating a cytokine profile for each sample.

Kit Content

Each kit contains a 96-well plate, featuring the relevant biomarker panel in each well, and all reagents required to perform testing.

Reagents Include:

  • Calibrator
  • Detection Mix
  • Substrates
  • Sample Diluents
  • Streptavidin HRP
  • Wash Buffer


All Quansys Q-Plex™ Multiplex and Singleplex assays require the use of the Q-View™ software to read and interpret the test results. A fully-functional, free trial version of the software is available to download, install, and use to analyze your first kit(s). At the end of the trial period, a purchased license is required to continue the use of the Q-View software.

Q-Plex arrays are developed and optimized to work with Q-View imagers. Quansys does not guarantee the results obtained from other imagers and not all imagers are compatible with Quansys Q-Plex arrays.

Other Equipment Recommendations

Publication Library

Search our publication library for all the peer-reviewed journals and research products that have utilized Q-Plex™ technology here.

In-Kit Certificate of Analysis

Search and download Certificates of Analysis for your Quansys Biosciences kits here.

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Not Finding What You Need?

Customize a Kit

Not seeing the biomarker or analyte you need? Customize your kit! The Q-Plex™ Custom Assays are fully quantitative ELISA-based Chemiluminescent assay kits allowing the concurrent measurement of up to 18 biomarkers or analytes.

Send in Your Samples

Choose up to 18 different biomarkers that will work best for your research. Send in your samples and let us run them for you. 

Contact a Representative

Have questions about the kit or interested in more information? Contact one of our representative to get all your questions answered. 

As researchers and problem solvers ourselves, we understand the value of sound data. We are proud to be a part of research that can better the world. We value the relationships, partnerships, and friendships that we have built with the people who trust and use our technology. We are committed to building these relationships. You can count on us to answer the phone and take time to thoroughly address questions or concerns about any of our products. In an industry that is reputed for grandiose claims, we trust that our quality standards and our customer service set us apart from the competition. If you’re happy with our products or if you think we can do something better, we hope you will let us know.