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Human IL-6 Analyte Overview

Human IL-6

Also known as Interleukin 6

Interleuken 6 (IL-6) has the capability to behave as both a pro-inflammatory cytokine and an anti-inflammation myokine. As a cytokine, it is secreted by macrophages in response to microbial activity, to  regulate the progression of fever through raising the temperature of muscle and fat, as well as crossing the blood-brain barrier to alter the body’s temperature set point in the hypothalamus. It acts through T cells and macrophages fighting infection and causing inflammation. As a myokine, it is released by contracting muscles during exercise, and inhibits the activity of pro-inflammatory agent TNF-alpha, reducing system-wide inflammation. In addition to its functions in the immune system, IL-6 aids in bone remodeling and maintenance by stimulating the production of osteoclasts. Clinically, IL-6 is implicated in the inflammatory and autoimmune symptoms of many diseases, including diabetes, asthma, atherosclerosis, depression, Alzheimer’s disease, rheumatoid arthritis, and many cancers. High levels of IL-6 are common in advanced cancer patients and are correlated with low survival rates. IL-6 has the ability to induce epigenetic modifications of gene transcription in the brain, and these IL-6 modifications are associated with schizophrenia and major depressive disorder.

Q-Plex™ Details

Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 3,000 – 4.11 pg/mL
Lower Limit of Detection (LLD): 31.47 pg/mL
Volume Required Per Well: Min. 25µL
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog #: 107749GR

Features & Requirements

  • Measure IL-6 in 2.25 hours
  • Sensitive, reproducible, and convenient 96-well format requiring low sample volumes
  • Requires the Q-View™ Imaging System for analysis

Assay Performance Characteristics

Precision

Intra-assay (n=20)
Average Intra-assay CV: 7%

Sample Concentration (pg/ml) %CV
Sample 1

594.53

8%
Sample 2 47.69 6%
Sample 3 16.35 8%

 

Inter-assay (n=15)
Average Inter-assay CV: 7%

Sample Concentration (pg/ml) %CV
Sample 1 2272.50 7%
Sample 2 291.94 9%
Sample 3 738.57 5%

 

Dilutional Linearity

Average percent linearity: 104%
Percent linearity range: 94 – 111%

Dilution Factor % Recovery
Serum HA Serum HB Serum HC Pooled Plasma
2 104% 111 103% 111%
4 107% 108% 102% 104%
8 101% 107% 94% 94%
human il-4 linearity

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Not seeing the biomarker or analyte you need? Customize your kit! The Q-Plex™ Custom Assays are fully quantitative ELISA-based Chemiluminescent assay kits allowing the concurrent measurement of up to 18 biomarkers or analytes.

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Choose up to 18 different biomarkers that will work best for your research. Send in your samples and let us run them for you. 

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