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NHP IL-4 Analyte Overview

NHP IL-4

Also known as Interleukin 4, B cell-stimulatory factor-1

Interleukin 4 (IL-4) is a cytokine that has pleiotropic effects during active immune responses. Its primary role is to induce differentiation of helper T-cells and active B cells. IL-4’s other roles include regulating immunity, upregulating MHC class II production, supporting the proliferation of epithelial cells, and downregulating the production of Th1 cells, macrophages, and some lines of dendritic cells.  IL-4 activates two different receptor complexes, one expressed on hematopoietic cells and another found on nonhematopoietic cells. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, eosinophils, and basophils. Its responsibilities include cell proliferation, immunoglobulin class switch (IgG4, IgE of human B cells), survival, priming and chemotaxis of mast cells, eosinophils, and basophils. Dysregulation of IL-4 is implicated in many autoimmune disorders, particularly the development of allergies. Overexpression of the cytokine is associated with tumor growth in many kinds of cancer. People infected with HIV often produce higher levels of IL-4. Human, mouse, and rat IL-4 are species-specific in their activities.

Typical Assay Characteristics

NHP IL-4 analyte graph
Precision Linearity
Inter-assay Intra-assay Range Average
16% 12% 95%-120% 109%

Q-Plex™ Details

Species: NHP
Assay Type: Sandwich
Sample Type: Serum/Plasma, Cell Supernatant
Assay Range: 60 – 0.082 pg/mL
Lower Limit of Detection (LLD): 0.041 pg/mL
Volume Required Per Well: Min. 25µL
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate

Features & Requirements

  • Data available as fast as 4 hours
  • Sensitive, reproducible, and convenient 96-well format requiring low sample volumes
  • Requires the Q-View™ Imaging System for analysis of multiplexed chemiluminescent planar-based arrays

Featured Q-Plex Products

Q-Plex™ NHP Anti-Inflammatory Panel 1 (7-Plex)

IL-1RA, IL-2Rα, IL-4, sIL-6R, IL-10, IL-13, TNFRII

Q-Plex™ NHP Cytokine Screen (16-Plex)

CXCL-1, GMCSF, IFNγ, IL-1ß, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-15, IL-17A, IL-18, TNFα

Not Finding What You Need?

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Not seeing the biomarker or analyte you need? Customize your kit! The Q-Plex™ Custom Assays are fully quantitative ELISA-based Chemiluminescent assay kits allowing the concurrent measurement of up to 18 biomarkers or analytes.

Send in Your Samples

Choose up to 18 different biomarkers that will work best for your research. Send in your samples and let us run them for you. 

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Have questions about the kit or interested in more information? Contact one of our representative to get all your questions answered. 

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