Q-Plex™ NHP Cytokine Screen (16-Plex)
Q-Plex NHP Cytokine Screen (16-Plex)
For measurement of CXCL-1, GMCSF, IFNγ, IL-1ß, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-15, IL-17A, IL-18, TNFα
- Species Specificity: NHP
- Validated Sample Type: Serum, Plasma
- Sample Volume Required: Min. 25 µL
- Estimated Assay Length: 4 hours
- Assay Type: Sandwich
- Detection Method: Chemiluminescent
- Multiplex Format: 96-Well Solid Plate
- Catalog #: 110933MB
| Analyte | Assay Type | Calibrator Range | Upper Limit of Quantification (ULOQ) | Lower Limit of Quantification (LLOQ) | Limit of Detection | Precision (Inter-assay) | Precision (Intra-assay) | Average Linearity |
|---|---|---|---|---|---|---|---|---|
| CXCL-1 | Sandwich | 2,000 - 2.74 (pg/mL) | 1,400 (pg/mL) | 3.57 (pg/mL) | 1.37 (pg/mL) | 8% | 4% | 94% |
| GMCSF | Sandwich | 6,000 – 8.23 (pg/mL) | 4,200 (pg/mL) | 10.7 (pg/mL) | 4.12 (pg/mL) | 8% | 8% | 111% |
| IFNγ | Sandwich | 1,000 – 1.37 (pg/mL) | 700 (pg/mL) | 1.78 (pg/mL) | 0.69 (pg/mL) | 8% | 5% | 118% |
| IL-1ß | Sandwich | 4,075 – 5.59 (µg/mL) | 2,852.5 (pg/mL) | 7.27 (pg/mL) | 2.79 (pg/mL) | 9% | 7% | 116% |
| IL-2 | Sandwich | 650 – 0.89 (µg/mL) | 455 (pg/mL) | 1.16 (pg/mL) | 0.45 (pg/mL) | 7% | 9% | 118% |
| IL-4 | Sandwich | 60 – 0.08 (pg/mL) | 42 (pg/mL) | 0.11 (pg/mL) | 0.04 (pg/mL) | 16% | 12% | 109% |
| IL-5 | Sandwich | 333 – 0.46 (pg/mL) | 233.1 (pg/mL) | 0.59 (pg/mL) | 0.23 (pg/mL) | 17% | 9% | 98% |
| IL-6 | Sandwich | 1,000 – 1.37 (pg/mL) | 700 (pg/mL) | 1.78 (pg/mL) | 0.69 (pg/mL) | 16% | 6% | 107% |
| IL-8 | Sandwich | 2,000 – 2.74 (pg/mL) | 1,400 (pg/mL) | 3.57 (pg/mL) | 1.37 (pg/mL) | 12% | 6% | 99% |
| IL-10 | Sandwich | 2,680 – 3.68 (pg/mL) | 1,867 (pg/mL) | 4.78 (pg/mL) | 1.84 (pg/mL) | 13% | 4% | 102% |
| IL-12p70 | Sandwich | 3,000 – 4.12 (pg/mL) | 2,100 (pg/mL) | 5.35 (pg/mL) | 2.06 (pg/mL) | 16% | 12% | 106% |
| IL-13 | Sandwich | 333 – 0.46 (pg/mL) | 233.1 (pg/mL) | 0.59 (pg/mL) | 0.23 (pg/mL) | 11% | 8% | 95% |
| IL-15 | Sandwich | 3,578 – 4.91 (pg/mL) | 2,504.6 (pg/mL) | 6.38 (pg/mL) | 2.45 (pg/mL) | 18% | 7% | 97% |
| IL-17A | Sandwich | 5,589 – 7.67 (pg/mL) | 3,912.3 (pg/mL) | 9.97 (pg/mL) | 3.83 (pg/mL) | 19% | 13% | 117% |
| IL-18 | Sandwich | 235 – 0.32 (pg/mL) | 151 (pg/mL) | 0.38 (pg/mL) | 0.16 (pg/mL) | 13% | 4% | 103% |
| TNFα | Sandwich | 250 – 0.34 (pg/mL) | 175 (pg/mL) | 0.45 (pg/mL) | 0.17 (pg/mL) | 11% | 3% | 98% |
Product Overview
The Q-Plex NHP Cytokine Screen (16-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing the concurrent measurement of 16 biomarkers or analytes. Multiple analyte measurements are achieved by adsorbing 16 distinct capture antibodies in a defined array to the bottom of each well of a 96-well plate. Our high-quality reagents help ensure the accuracy and precision of your results.
Using only 25 µL of sample per well, up to 80 samples can be assayed for all 16 markers in the panel within 4 hours. The Q-Plex NHP Cytokine Screen (16-Plex) provides researchers with an easy-to-use and cost-effective means of generating a cytokine profile for each sample.
Product Documentation
Kit Content
Each kit contains a 96-well plate, featuring the relevant biomarker panel in each well, and all reagents required to perform testing.
Reagents Include:
- Sample Diluent
- Calibrator
- Detection Mix
- Streptavidin HRP
- Wash Buffer
- Substrates
Processing
All Quansys Q-Plex™ Multiplex and Singleplex assays require the use of the Q-View™ software to read and interpret the test results. A fully-functional, free trial version of the software is available to download, install, and use to analyze your first kit(s). At the end of the trial period, a purchased license is required to continue the use of the Q-View software.
Q-Plex arrays are developed and optimized to work with Q-View imagers. Quansys does not guarantee the results obtained from other imagers and not all imagers are compatible with Quansys Q-Plex arrays.
Order Your Kit Today!
Not Finding What You Need?
Customize a Kit
Not seeing the biomarker or analyte you need? Customize your kit! The Q-Plex™ Custom Assays are fully quantitative ELISA-based Chemiluminescent assay kits allowing the concurrent measurement of up to 18 biomarkers or analytes.
Send in Your Samples
Choose up to 18 different biomarkers that will work best for your research. Send in your samples and let us run them for you.
Contact a Representative
Have questions about the kit or interested in more information? Contact one of our representative to get all your questions answered.
As researchers and problem solvers ourselves, we understand the value of sound data. We are proud to be a part of research that can better the world. We value the relationships, partnerships, and friendships that we have built with the people who trust and use our technology. We are committed to building these relationships. You can count on us to answer the phone and take time to thoroughly address questions or concerns about any of our products. In an industry that is reputed for grandiose claims, we trust that our quality standards and our customer service set us apart from the competition. If you’re happy with our products or if you think we can do something better, we hope you will let us know.