Human HGF Analyte Overview

Human HGF

Also known as Hepatocyte Growth Factor, Scatter Factor

Hepatocyte growth factor (HGF), also known as scatter factor (SF), is a paracrine factor involved in cellular growth, motility, and morphogenesis. It plays an important role in embryonic development, wound healing, and adult organ maintenance, particularly for muscle cells. HGF causes epithelial cells, hepatocytes, keratinocytes, endothelial cells, melanocytes, and chondrocytes to proliferate and migrate during tissue formation. During early organ formation, it promotes morphogenesis by inducing cell scattering and regulating organization into structures. In adults, HGF is secreted at sites of tissue damage. High levels of serum HGF is present in conditions such as liver damage, acute kidney failure, type 1 diabetes, obesity, cancer, and myocardial infarction. High levels of HGF is are often present in the synovial fluid of patients with rheumatoid arthritis. HGF is currently being explored as a potential therapeutic for tissue repair in cardiac disease patients.

HGF Q-Plex™ details

Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 14,000 – 19..20 pg/ml
Lower Limit of Detection (LLD): 7.20 pg/ml
Volume Required Per Well: Min 25µl
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog No: 464449HU

N

Measure HGF in 2.25 hours

N

Sensitive, reproducible, and convenient 96-well format requiring low sample volumes

N

Requires the Q-View™ Imaging System for analysis

HGF Assay Performance Characteristics

Precision

Intra-assay (n=20)
Average Intra-assay CV: 5%

Sample Concentration (pg/ml) %CV
Sample 1

3297.11

5%
Sample 2 223.22 8%
Sample 3 71.37 2%

 

Inter-assay (n=15)
Average Inter-assay CV: 10%

Sample Concentration (pg/ml) %CV
Sample 1 456.20 11%
Sample 2 268.67 11%
Sample 3 3211.29 7%

 

Dilutional Linearity

Average percent linearity: 96%
Percent linearity range: 85 – 107%

Dilution Factor % Recovery
Serum HB Serum HC PI Plasma Stripped Serum
2 99% 92% 103% 99%
4 95% 87% 102% 107%
8 88% 85% 96% 99%
Human HGF Linearity

Q-Plex Multiplexed Products featuring HGF

Q-Plex Products featuring HGF

Human Multiplex Kits

Human Angiogenesis (9-plex)

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