Human IL-12p70 Analyte Overview
Human IL-12p70
Also known as CLMF p35, interleukin 12, p35, interleukin 12A, NKSF1, Active Heterodimer of Interleukin-12
IL-12p70 Q-Plex™ details
Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 3,000 – 5.3 pg/ml
Lower Limit of Detection (LLD): 2.65 pg/ml
Volume Required Per Well: Min 25µl
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog No: 460449HU
Measure IL-12p70 in 2.25 hours
Sensitive, reproducible, and convenient 96-well format requiring low sample volumes
Requires the Q-View™ Imaging System for analysis
IL-12p70 Assay Performance Characteristics
Precision
Intra-assay (n=20)
Average Intra-assay CV: 7%
| Sample | Concentration (pg/ml) | %CV |
| Sample 1 |
570.17 |
9% |
| Sample 2 | 50.53 | 5% |
| Sample 3 | 15.66 | 8% |
Inter-assay (n=15)
Average Inter-assay CV: 7%
| Sample | Concentration (pg/ml) | %CV |
| Sample 1 | 55.64 | 9% |
| Sample 2 | 410.85 | 7% |
| Sample 3 | 704.21 | 6% |
Dilutional Linearity
Average percent linearity: 103%
Percent linearity range: 98 – 110%
| Dilution Factor | % Recovery | |||
| Serum HH | Pooled Plasma | Pooled Serum | Serum HF | |
| 2 | 105% | 107% | 98% | 108% |
| 4 | 100% | 102% | 100% | 103% |
| 8 | 108% | 100% | 99% | 110% |
Q-Plex Multiplexed Products featuring IL-12p70
Q-Plex Products featuring IL-12p70
Rat Multiplex Kits
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