Human IL-1α Analyte Overview

Human IL-1a

Also known as Interleuken 1 alpha, fibroblast-activating factor (FAF), B-cell-activating factor (BAF), leukocyte endogenouse mediator (LEM), epidermal cell-derived thymocyte-activating factor (ETAF), serum amyloid A inducer of hepatocyte-stimulating factor (HS), catabolin, hemopoetin-1 (H-1), endogenous pyrogen (EP), osteoclast-activating factor (OAF), proteolysis-inducing factor (PIF), IL1a, IL-1a, mouse interleukin 1a

Interleuken 1 alpha (IL-1a or IL-1α), also known as hematopoietin 1, is a cytokine that plays a role in inflammation, fever, and sepsis. It is produced by macrophages, neutrophils, and endothelial and epithelial cells. In the skin, IL-1a is regularly produced at high levels and helps maintain skin barrier function against pathogens as well as overall skin condition. As part of the immune response, its production is by microbial exposure. It is responsible for releasing inflammatory mediators and acute phase proteins. The severity of inflammation, fever, and sepsis are associated with circulating IL-1a levels. IL-1a inhibitors are a potential target for therapeutics to treat and prevent these conditions as well as mediate skin conditions such as acne. Additionally, IL-1a is part of the pathway that activates tumor necrosis factor-alpha and helps kill some kinds of cancer cells. It also supports stem cell expansion and osteoclast formation following bone marrow transplant.

IL-1α Q-Plex™ details

Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 4,000 – 5.49 pg/ml
Lower Limit of Detection (LLD): 5.43 ng/ml
Volume Required Per Well: Min 25µl
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog No: 465449HU

    N

    Measure IL-1alpha in 2.25 hours

    N

    Sensitive, reproducible, and convenient 96-well format requiring low sample volumes

    N

    Requires the Q-View™ Imaging System for analysis

    IL-1α Assay Performance Characteristics

    Precision

    Intra-assay (n=20)
    Average Intra-assay CV: 3%

    Sample Concentration (pg/ml) %CV
    Sample 1 608.31 2%
    Sample 2 48.38 2%
    Sample 3 16.97 4%

     

    Inter-assay (n=15)
    Average Inter-assay CV: 6%

    Sample Concentration (pg/ml) %CV
    Sample 1 826.63 7%
    Sample 2 646.36 3%
    Sample 3 51.78 6%

     

    Dilutional Linearity

    Average percent linearity: 86%
    Percent linearity range: 81 – 91%

    Dilution Factor % Recovery
    Serum HA Pooled Serum Pooled Plasma Plasma HD
    2 81% 90% 87% 82%
    4 89% 91% 86% 83%
    8 89% 86% 83% 86%
    IL-1a Linearity

    Q-Plex Multiplexed Products featuring IL-1α

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