Human IL-1β Analyte Overview

Human IL-1b

Also known as Interleukin 1 beta, IL1β, leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor, pre-interleukin 1 beta, catabolin

Interleukin 1 beta (IL-1β) is a cytokine involved in pro-inflammatory responses. It is produced by activated macrophages, natural killer cells, monocytes, and neutrophils in response to wounds, infections, or microbial endotoxins and is processed to its active state by caspase 1. It mediates inflammatory responses and plays a role in cell proliferation, apoptosis, and differentiation. IL-1β inflammation within the central nervous system is known to result in pain and hypersensitivity. High levels of IL-1β are associated with autoinflammatory diseases, particularly the group of rare heterozygous dominant diseases known as cryopyrin-associated periodic syndromes, that often affect the skin, joints, eyes, and nervous system. Inhibition of IL-1β treats these diseases and eases symptoms in common conditions such as type 2 diabetes, cardiac failure, gout, and rheumatoid arthritis. Dysregulated IL-1β is associated with tumor formation in a wide variety of cancers.

IL-1β Q-Plex™ details

Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma, Heparin Plasma
Assay Range: 12,226 – 21.80 pg/ml
Lower Limit of Detection (LLD): 10.90 pg/ml
Volume Required Per Well: Min 25µl
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog No: 465549HU


    Measure IL-1beta in 2.25 hours


    Sensitive, reproducible, and convenient 96-well format requiring low sample volumes


    Requires the Q-View™ Imaging System for analysis

    IL-1β Assay Performance Characteristics


    Intra-assay (n=20)
    Average Intra-assay CV: 6%

    Sample Concentration (pg/ml) %CV
    Sample 1 1333.12 6%
    Sample 2 103.03 7%
    Sample 3 35.49 6%


    Inter-assay (n=15)
    Average Inter-assay CV: 8%

    Sample Concentration (pg/ml) %CV
    Sample 1 2821.92 5%
    Sample 2 1091.31 8%
    Sample 3 175.1 10%


    Dilutional Linearity

    Average percent linearity: 94%
    Percent linearity range: 86 – 103%

    Dilution Factor % Recovery
    Serum HA Serum HB Serum HC Pooled Plasma
    2 92% 95% 86% 91%
    4 100% 95% 103% 98%
    8 94% 98% 91% 87%
    human il1b dilution linearity

    Q-Plex Multiplexed Products featuring IL-1β

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