Human IP-10 Analyte Overview

Human IP-10

Also known as Interferon Gamma-Induced Protein 10, CXCL10, C-X-C Motif Chemokine 10

Interferon gamma-induced protein 10 (IP-10), also known as C-X-C motif chemokine 10 (CXCL10) or small-inducible cytokine B10, is a small cytokine with diverse roles in the immune system. Many cell types release IP-10 in response to stimulation from IFN-γ. It has chemotactic properties for activated T cells, monocytes, macrophages, natural killer cells, and dendritic cells. It promotes the adhesion of T cells to endothelial cells and has strong anti-tumor properties. It inhibits bone marrow colony formation and angiogenesis. IP-10 expression has been associated with HIV-1 infection, where it contributes to the accumulation of activated T cells in the cerebrospinal fluid compartment of infected individuals. The retroviral transactivator, HIV-1 Tat, is a potent inducer of IP-10 expression in astrocytes. IP-10 expression has also been shown to be significantly elevated in astrocytes within the brains of Alzheimer’s disease patients, where its expression is associated with senile plaques. Higher levels of IP-10 are documented in Hepatitis C patients who respond poorly to antiviral treatment.

IP-10 Q-Plex™ details

Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 2,984 – 4.10 pg/ml
Lower Limit of Detection (LLD): 4.03 pg/ml
Volume Required Per Well: Min 25µl
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog No: 466849HU

N

Measure IP-10 in 2.25 hours

N

Sensitive, reproducible, and convenient 96-well format requiring low sample volumes

N

Requires the Q-View™ Imaging System for analysis

IP-10 Assay Performance Characteristics

Precision

Intra-assay (n=20)
Average Intra-assay CV: 5%

Sample Concentration (pg/ml) %CV
Sample 1

770.42

6%
Sample 2 50.73 5%
Sample 3 16.40 4%

 

Inter-assay (n=15)
Average Inter-assay CV: 6%

Sample Concentration (pg/ml) %CV
Sample 1 146.35 3%
Sample 2 172.85 8%
Sample 3 243.14 9%

 

Dilutional Linearity

Average percent linearity: 100%
Percent linearity range: 88 – 119%

Dilution Factor % Recovery
Activated Plasma Pooled Plasma Serum HE Cell Culture Supernatant
2 110% 104% 119% 107%
4 98% 96% 88% 98%
8 102% 98% 89% 93%
human IP-10 linearity

Q-Plex Multiplexed Products featuring IP-10

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