Human PDGF-BB Analyte Overview

Human PDGF-BB

Also known as PDGF, PDGF-BB Platelet Derived Growth Factor

Platelet-derived growth factor (PDGF) is a growth factor that mediates growth and cell division. It is a critical regulator of early embryonic development, where it drives the growth of the mesenchyme and later tissue remodeling and morphogenesis. In adults, PDGF promotes blood vessel formation and maintenance, wound healing, survival and regeneration of neurons, and induces of mitosis in connective tissue cells. In the immune system it stimulates neutrophil phagocytosis and inhibits natural killer cell activity. PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. The presence of this chemokine is common at the site of healing in damaged tissue, especially connective tissue, where it stimulates collagen synthesis. Clinically, PDGF is often used to heal ulcers, stimulate recovery following orthopedic surgery, and promote soft tissue healing.

PDGF-BB Q-Plex™ details

Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 4,000 – 5.50 pg/ml
Lower Limit of Detection (LLD): 2.20 pg/ml
Volume Required Per Well: Min 25µl
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog No: 468649HU

N

Measure PDGF-BB in 2.25 hours

N

Sensitive, reproducible, and convenient 96-well format requiring low sample volumes

N

Requires the Q-View™ Imaging System for analysis

PDGF-BB Assay Performance Characteristics

Precision

Intra-assay (n=20)
Average Intra-assay CV: 8%

Sample Concentration (pg/ml) %CV
Sample 1

889.32

10%
Sample 2 40.79 10%
Sample 3 12.83 4%

 

Inter-assay (n=15)
Average Inter-assay CV: 11%

Sample Concentration (pg/ml) %CV
Sample 1 1020.78 10%
Sample 2 1321.93 13%
Sample 3 678.42 9%

 

Dilutional Linearity

Average percent linearity: 98%
Percent linearity range: 91 – 104%

Dilution Factor % Recovery
Serum HB Serum HD Pooled Plasma Stripped Serum
2 94% 101% 91% 101%
4 102% 91% 104% 104%
8 100% 97% 94% 99%
Human PDGF-BB Linearity

Q-Plex Multiplexed Products featuring PDGF-BB

Q-Plex Products featuring PDGF-BB

Human Multiplex Kits

Human Angiogenesis (9-plex)

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