Human IL-2 Analyte Overview

Human IL-2

Also known as Interleukin 2

Interleukin-2 (IL-2) cytokine signaling molecule in the immune system that mediates the activity of white blood cells, particularly lymphocytes, during an immune response. IL-2 promotes the differentiation of T cells into memory T cells after exposure to a novel pathogen, making it a critical component of the active immune system and long-term immunity to disease. In the thymus, IL-2 supports the formation of regulatory T cells, which work to prevent autoimmune diseases by eliminating overactive immune cells. Since IL-2 helps increase immune cell production and regulate large scale activities of the immune system, it is commonly used as a cancer treatment for patients with certain kinds of cancers, including kidney and skin cancers.

Q-Plex™ Details

Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 5,213 – 7.15 pg/mL
Lower Limit of Detection (LLD): 4.12 pg/mL
Volume Required Per Well: Min. 25µL
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog #: 465749HU

Features & Requirements

  • Measure IL-2 in 2.25 hours
  • Sensitive, reproducible, and convenient 96-well format requiring low sample volumes
  • Requires the Q-View™ Imaging System for analysis

Assay Performance Characteristics


Intra-assay (n=20)
Average Intra-assay CV: 5%

Sample Concentration (pg/ml) %CV
Sample 1 510.82 4%
Sample 2 35.56 4%
Sample 3 12.15 7%


Inter-assay (n=15)
Average Inter-assay CV: 6%

Sample Concentration (pg/ml) %CV
Sample 1 1916.94 6%
Sample 2 139.65 10%
Sample 3 488.43 3%


Dilutional Linearity

Average percent linearity: 99%
Percent linearity range: 82 – 110%

Dilution Factor % Recovery
Serum HA Serum HB Serum HC Pooled Plasma
2 87% 93% 82% 93%
4 108% 101% 103% 107%
8 107% 110% 98% 97%
Human il-2 linearity

Not Finding What You Need?

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Not seeing the biomarker or analyte you need? Customize your kit! The Q-Plex™ Custom Assays are fully quantitative ELISA-based Chemiluminescent assay kits allowing the concurrent measurement of up to 18 biomarkers or analytes.

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Choose up to 18 different biomarkers that will work best for your research. Send in your samples and let us run them for you. 

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